UNCLASSIFIED//FOR OFFICIAL USE ONLY

UNCLASSIFIED//FOR OFFICIAL USE ONLY

UNCLASSIFIED // FOR OFFICIAL USE ONLY

UNCLASSIFIED // FOR OFFICIAL USE ONLY: The information in this document is the property of the Federal Bureau of Investigation and may be distributed to government (federal, state, tribal, or local) employees and contractors with a need to know. It contains

information that may be exempt from public release under the Freedom of Information Act (5 U.S.C. 552). Dissemination to the public, the media, or other personnel who do not have a valid need to know without prior approval of an authorized FBI official is

prohibited. Precautions must be taken to ensure this information is controlled, stored, handled, transmitted, distributed, and disposed of in a manner that precludes unauthorized access.

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CODIS and NDIS Update

Douglas Hares, Ph.D.

NDIS Custodian

CODIS Unit

September 24, 2019

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Disclaimer

Names of commercial manufacturers are provided for identification purposes only, and inclusion does not imply endorsement of the manufacturer, or its products or services by the FBI. The views expressed are those of the author(s) and do not necessarily reflect the official policy or position of the FBI or the U. S. Government.

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NDIS Statistics

465,270 Investigations Aided

348,942 Offender HitsCrime scene to offender within a state

53,679 National Offender HitsCrime scene in one state to an offender in another state

73,182 Forensic HitsCrime scene to crime scene

*Numbers represent confirmed matches where CODIS helped provide new information to a case

Through July 31, 2019Cumulative Totals Through July 31, 2019

Category Total Number

of Profiles

Convicted Offender 13,770,907

Multi-Allelic Offender 260

Arrestees 3,643,513

Detainees 28,144

Legal 102,153

Forensic 716,854

Forensic Mixture 92,835

Forensic Partial 145,265

Forensic Targeted 5,093

Total Offender 17,544,977

Total Forensic 960,047

Total Profiles 18,505,024

Number of Profiles added in 2018:

497,940 Convicted Offender Profiles

440,314 Arrestee Profiles

84,059 Forensic Profiles

48,814 Investigations Aided

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NDIS Missing Persons Statistics

Category Total Number of

Profiles

Missing Person 1,957

Relatives of Missing

Person25,902

Unidentified Human

(Remains)12,495

Total Profiles 40,354

Pedigree Trees 14,835

Through July 31, 2019*

1,308 Putative Identifications•Probative associations

made within a single state

411 National Putative Identifications•Probative associations

made between states

*Missing Persons Metrics officially tracked beginning in

January 2012

Cumulative Totals Through July 31, 2019

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Investigations Aided by State

The “Investigations Aided” is a metric that tracks the number of criminal investigations where

CODIS has added value to the investigative process.

Through July 2019

465,270 Investigations Aided in 50 States, 2 Federal Laboratories, Washington, DC, and Puerto

Rico

11,712

(MD)

409

7,775

4,962 (CT)

233 (DE)

2,023 (FBI)

217 (US Army)

178

5,453

(NJ)

(MA)

(NH)

5,609

9,208

4,770

645

45,185 (FL)

(HI)751

16,175

(RI)563

546 (VT)4,344

8,617

175

445

78,967

891

12,319 5,605

10,233

26,801

11,530

8,471

25,181

2,773

5,505

8,429

387

140

8,987

847

15,802

9,748

3,630

3,751

6432,813

34,459

4,793

5,353

1,043

947

8,425

29,690

6,465

Puerto Rico

48

(DC)599

(MI)

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New version of the NDIS

Operational Procedures

effective May 1, 2019

Added NGS/MPS section

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NDIS Procedures

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NEW Section 4.4

– To provide Standards for Acceptance of Next Generation Sequencing

DNA Records at NDIS

– DNA records must be generated in accordance with:

Addendum to the “SWGDAM Interpretation Guidelines for Autosomal

STR Typing by Forensic DNA Testing Laboratories” to Address Next

Generation Sequencing;

SWGDAM Interpretation Guidelines for Mitochondrial DNA Analysis by

Forensic DNA Testing Laboratories; and

SWGDAM Validation Guidelines for DNA Analysis Methods.

2019: NDIS Procedures - NGS

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NEW Section 4.4

Next generation sequencing kits produce DNA results that include the CODIS

Core Short Tandem Repeat (STR) Loci, in addition to new STR loci, Y

chromosomal STRs (Y STRs), X chromosomal STRs (X STRs), mtDNA, and

identity Single Nucleotide Polymorphisms (SNPs). The CODIS software is not

capable of storing, searching, or maintaining information on X STRs or identity

SNPs13 and thus, any DNA results generated on X STRs or identity SNPs shall

not be entered into NDIS. Only DNA records relating to the required CODIS

Core Loci and NDIS accepted loci as specified in Appendix D shall be

uploaded, stored and searched at NDIS.14

2019: NDIS Procedures - NGS

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Two new NGS Kits Accepted at NDIS

– PowerSeq™ CRM Nested System (for MtDNA data)

– ForenSeq™ DNA Signature Prep* (for STR and Y STR

data)

NGS Kits Accepted at NDIS

*A laboratory shall determine if evaluation of locus specific analytical and stochastic

thresholds are necessary as part of its validation of the kit in accordance with manufacturer

recommendations. For example, for the ForenSeq™ DNA Signature Prep Kit, the

manufacturer recommends using caution for interpretation of DYS392 and D22S1045 (see

https://verogen.com/wp-content/uploads/2018/08/ForenSeq-DNA-Prep-Guide-

VD2018005-A.pdf at pages 32, 35, and 38-40).

2019: New Table for NGS Kits Accepted at NDIS

NGS Kits Accepted at NDIS

Manufacturer Kit Name Technology

Promega PowerSeq™ CRM Nested System (Catalog #

AX5810)

MtDNA

Verogen ForenSeq™ DNA Signature Prep Kit (TG-

450-1001/TG-450-1002)*

STR, YSTR

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Third NGS kit approved at NDIS on 6-25-2019

NGS Kits Accepted at NDISManufacturer Kit Name Technology

Promega PowerSeq™ CRM Nested System (Catalog # AX5810) MtDNA

Thermo Fisher

Scientific

Applied Biosystems™ Precision ID mtDNA Whole Genome Panel

(Catalog # A30938)**

MtDNA***

Verogen ForenSeq™ DNA Signature Prep Kit (TG-450-1001/TG-450-

1002)*

STR, YSTR

*A laboratory shall determine if evaluation of locus specific analytical and stochastic thresholds are necessary as part of its validation of the kit in accordance with manufacturer recommendations. For example, for the ForenSeq™ DNA Signature Prep Kit, the manufacturer recommends using caution for interpretation of DYS392 and D22S1045 (see https://verogen.com/wp-content/uploads/2018/08/ForenSeq-DNA-Prep-Guide-VD2018005-A.pdf at pages 32, 35, and 38-40).**A laboratory must evaluate homopolymeric regions as part of its validation. Laboratories planning to use the

Applied Biosystems™ Precision ID mtDNA Whole Genome Panel in conjunction with the applicable instrumentation

must exercise care in developing interpretation parameters for the homopolymeric regions.

*** Only mtDNA control region data is approved for upload to NDIS.

Currently posted on the CODIS and NDIS Fact Sheet on FBI.gov and will

be added to the 2020 version of the NDIS procedures

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For review and planning purposes, the 2020 QAS documents are available

on the SWGDAM website (https://www.swgdam.org):

– 2020 Databasing and Forensic QAS

– 2020 Databasing and Forensic QAS Audit Documents

– 2020 QAS Guidance Document

– Comparison tables highlighting the differences between the current QAS and the

approved 2020 revisions

Please keep in mind that the current Databasing and Forensic QAS will

remain in effect through June 30, 2020 and all QAS audits conducted

through June 30, 2020 will be subject to the current QAS, available at:

– https://www.fbi.gov/services/laboratory/biometric-analysis/codis

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2020 QAS Documents

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2020 QAS Documents

www.swgdam.org

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Targeting Computer Based Training (CBT) to be released late

January 2020

Web Based QAS Q&A Sessions: February 2020 – October 2020

– Pre-requisite: QAS CBT

– Monthly sessions for NDIS labs, private labs, and accrediting bodies

(ANAB & A2LA)

– Discussion and Q&A

Q&A will be collected for creating QAS FAQ webpage

Questions can be sent to QAS@fbi.gov

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Proposed 2020 QAS Training

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CODIS Web Site

• https://www.fbi.gov/services

/laboratory/biometric-

analysis/codis

• FBI’s Quality Assurance

Standards

• NDIS Operational

Procedures

• Rapid DNA

• CODIS and NDIS Fact Sheet

– frequently asked questions

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Contact Info

FBI Laboratory, Quantico Virginia

CODIS Unit

703 632 8315 Office 703 632 8305 FAX

drhares@fbi.gov

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