PCB 3233 Practice Exam 2: Chapters 3 and 4

*Please note that these questions are ONLY here to help. They are NOT actual test questions. Some, all, or none of these topics may be on your exam. Professor Weigel did not review this. Do NOT use this as your ONLY study resource, continue to review PowerPoints, recordings, textbook, notes, light board videos, etc*

1. When a pathogen invades a tissue, which cell of the immune system is more likely to

respond first? a. Neutrophil b. T Cell c. B Cell d. Macrophage

2. The receptor for C3b is?

a. CR1 b. CR2 c. CR3 d. All of the above

3. On the surface of a pathogen, iC3b can still turn into a C3 convertase of the alternative

pathway a. True b. False

4. The function of IL-8 is to:

a. Activate macrophages b. Attract neutrophils c. Recruit NK Cells d. To produce heat.

5. Pyogenic refers to

a. Heat producing b. Pus producing c. A & B d. None of the above

6. What extracellular proteins does TLR need to be able to start the cytokine producing

protein cascade a. MD2 b. CD14 c. LBP d. All of the above

Chpt 3 Slide6

Chpt3Slide7

Chpt2 Slide31 FactorHand I

Chpt3 Slide 17

Chpt I Slide 32

Chpt 3 Slide 15 TLR4

7. Selectins bind to vascular addressins. a. True b. False

8. Select the one in the correct order.

a. Diapedesis - Rolling adhesion - Migration - Tight Binding. b. Rolling adhesion - Migration -Tight Binding - Diapedesis c. Rolling Adhesion - Tight Binding - Diapedesis – Migration d. Tight Binding - Rolling Adhesion – Diapedesis – Migration

9. Systemic distribution of TNF α in blood circulation may prevent:

a. respiratory burst b. interferon response c. septic shock d. hypertension

10. Systemic distribution of C3a and C5a may cause

a. Anaphylaxis b. Fever c. Respiratory burst d. A & C

11. Identify the mismatched pair: a. ICAM-1 : LFA-1 b. CD14:LPS c. NK Cells : INF - gamma d. Selectin : Integrin

12. C4b2a3b is the C3 convertase of the classical pathway:

a. True b. False

13. Which of the following PAMP's is recognized by TLR4?

a. Flagellin b. Double Stranded DNA Virus c. Single Stranded RNA Virus d. LPS

14. Which of the following cytokines are called pyrogens? a. TNFα, b. IL-6, c. IL-1 d. A, B & C

Chpt 3 Slide15

Chpt 3 Slide32

Chpt 3Slide19

Chpt 2 slide 27

Chpt 3 Slide 53

Chpt 3 Slide 12

Chpt 3 Slide 42

15. INF α and INF ß are produced by which cells? a. Only NK Cells b. Only T cells c. All nucleated cells d. A & B

16. The Ig’s variable regions of the heavy and light chain contain a. Complimentarity determining regions b. Hypervariable regions c. Framework region d. A and B

17. If a B cell switched from IgM to IgG3, can the B cell make IgD if needed?

a. Yes b. No

18. IgM has greater avidity than IgG.

a. True b. False

19. Which of the following isotype did a newborn child gain from their mom and stays with them for about 12-16 months?

a. IgG b. IgM c. IgA d. IgE

20. Which of the following are not in monomeric form when secreted? a. IgG b. IgA c. IgM d. B & C

21. One binding site of any Ig is composed by

a. 1 Hypervariable region b. 2 Hypervariable regions c. 3 Hypervariable regions d. 6 Hypervariable regions

22. Antibodies can:

a. Act as opsonins b. Neutralize c. Act as toxins and kill pathogens directly

Chpt 3 Slide 57

Chpt4 Slide32

Chpt 4 Slide69

Chpt4 Slides 10 I 21

Chpt4 Slide 89

Chpt4 Slides18,2026

Chpt 4 Slide 31

Chpt4 Slide 86

d. A and B

23. Which sequence of genes are contained in the germ-line DNA for the Heavy Chain? a. V and J b. V, D and J c. Only V and D d. Only D and J

24. The third hypervariable region of light chain (CDR3) is determined by:

a. V segments b. J segments c. V/J junction d. D segments

25. RAG-1 and RAG-2 enzymes would be present in which cells?

a. Maturing B ells b. Maturing T cells c. Dendritic cells to rearrange their MHC molecules d. A and B

26. Which is an important characteristic of RSS's flanking each V, D and J gene segments?

a. 12/23 bp or one-to-two rule b. 12/24 bp or one-to-two rule c. Having only heptamers in between each gene segment. d. Having only monomers in between each gene segment.

27. Random addition of nucleotides in the spaces between gene segments of germline DNA is known as:

a. Gene diversity. b. Light chain variability c. Junctional diversity. d. None of the above

28. How many attempts do you have to rearrange the heavy chain during somatic

recombination a. 1 b. 2 c. 3 d. 4

29. Whether an Ig is secreted or membrane bound is determined during: a. Somatic Recombination b. Somatic Hypermutation c. RNA splicing

Chpt4Slide46

Chpt 4 Slide 51

Chpt4 slide59

Chpt4Slides57,6061

Chpt4 Slide 63

Chpt4 Slide69

d. DNA Splicing

30. The MAC can be formed by: a. C3b2Bb b. C4b2a3b c. iC3b d. A & B

31. The difference between a membrane-bound and secreted Ig is:

a. The N-terminus on a secreted Ig will be hydrophobic. b. The C-terminus on a secreted Ig will be hydrophobic. c. The N-terminus on a membrane-bound Ig will be hydrophilic. d. The C-terminus on a membrane-bound Ig will be hydrophobic.

32. Somatic hypermutation occurs before somatic recombination.

a. True b. False

33. Somatic Hypermutation

a. Does not mutate the Ig b. Introduces point mutations in the C-domain. c. Introduces point mutations in the V-domain. d. B & C

34. Enzyme/s involved in somatic hypermutations is/are: a. RAG-1 b. RAG-2 c. AID d. RNA Polymerase

35. When a B cell is activated, the first Ig to be secreted is:

a. IgG b. IgA c. IgM d. IgE

36. Somatic Hypermutation will also lead to affinity maturation.

a. True b. False

37. Which lymphocyte has the darkest stain (very basic), and is usually involved in

allergic reactions? a. Neutrophils

Chpt 2 Slide 22

Chpt 4 Slides72,76

Chpt4 Slide 77

Chpt4 Slide 77

Chpt4 Slide 74

Chpt4 Slide81

b. Basophils c. T Cells d. None of the above

38. The protein that initiates the cascade in the classical pathway is:

a. C1q b. C3b c. C1s d. A & C

39. You would expect to find RAG 1 and 2 being expressed in B cell being activated in a

second lymphoid tissues a. True b. False

40. The _ region determines the fate of the antigen and has less variation in amino acid

sequence. a. V b. C c. D d. L

Chpt 3 Slide 54

Chpt4 slide 59

Chpt4 Slide 7

Matching Match each of the following with the correct statement.

1. IgG 2. IgA 3. IgM 4. IgE 5. ICAM-1 6. IL-6 7. IgD 8. INF-γ 9. TNF-α 10. NFkB 11. IL-12 12. CXCL8

a. Inflammatory cytokine that induces the expression of adhesive proteins on the luminal side of endothelial cell

b. Chemokine that attracts neutrophils c. Activates transcription of genes for inflammatory cytokines d. The only immunoglobulin that can cross the placenta e. Immunoglobulin like molecule that is involved in tight binding between

leukocytes and endothelial cells. f. make temperature rise in the infected tissue g. Expressed by NK cells and T Cells h. Most abundant immunoglobulin present in external secretions i. Involved in allergies j. Recruits and activates NK cells k. Highest avidity l. Is expressed on a mature B cell

In

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