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CHMI 2227 - E.R. Gauthier, Ph.D. 3
Enzyme catalysisBasic reaction mechanisms
General acid/base catalysis:
Three main types of catalysis are found:
Covalent catalysis: Metal ion catalysis:
CHMI 2227 - E.R. Gauthier, Ph.D. 4
Enzyme catalysisGeneral acid/base catalysis
Chymotrypsin
O
OHR
HOR
+
H
CHMI 2227 - E.R. Gauthier, Ph.D. 8
Enzyme catalysis- Enzymes often combine two catalytic strategies
General Acid/base catalysis
Covalent catalysis
CHMI 2227 - E.R. Gauthier, Ph.D. 13
Example of enzyme catalysis1. Chymotrypsin - specificity
Ser195 Ser195
CHMI 2227 - E.R. Gauthier, Ph.D. 14
Example of enzyme catalysis1. Chymotrypsin - catalysis
Catalytic triad
Substrate
C-ter part of the protein
N-ter part of the protein
Peptide bond cleavage
Enzyme regeneration
Covalent intermediate
CHMI 2227 - E.R. Gauthier, Ph.D. 16
Example of enzyme catalysisSimilarities between different proteases
CHMI 2227 - E.R. Gauthier, Ph.D. 17
Example of enzyme catalysisSimilarities between different proteases
CHMI 2227 - E.R. Gauthier, Ph.D. 22
Example of enzyme catalysis3. Lysozyme
Sugar portion of peptidoglycan