CGC GCG TTG ACA TTG ATT ATT GAC TAG TTA TTA ATA GTA

Tuesday, September 6, 2011 9:18 AM Page 1 of 13YbeJ253.L1LVL2NP.pMinDis

hCMV promoter/enhancer

BssHIIMauBI

CGC GCG TTG ACA TTG ATT ATT GAC TAG TTA TTA ATA GTA ATC AAT TAC GGG GTC5'

54


ATT AGT TCA TAG CCC ATA TAT GGA GTT CCG CGT TAC ATA ACT TAC GGT AAA TGG5'

108


CCC GCC TGG CTG ACC GCC CAA CGA CCC CCG CCC ATT GAC GTC AAT AAT GAC GTA5'

162


TGT TCC CAT AGT AAC GCC AAT AGG GAC TTT CCA TTG ACG TCA ATG GGT GGA CTA5'

216


NdeI

TTT ACG GTA AAC TGC CCA CTT GGC AGT ACA TCA AGT GTA TCA TAT GCC AAG TAC5'

270


GCC CCC TAT TGA CGT CAA TGA CGG TAA ATG GCC CGC CTG GCA TTA TGC CCA GTA5'

324


SnaBI

CAT GAC CTT ATG GGA CTT TCC TAC TTG GCA GTA CAT CTA CGT ATT AGT CAT CGC5'

378


TAT TAC CAT GGT GAT GCG GTT TTG GCA GTA CAT CAA TGG GCG TGG ATA GCG GTT5'

432


TGA CTC ACG GGG ATT TCC AAG TCT CCA CCC CAT TGA CGT CAA TGG GAG TTT GTT5'

486


TTG GCA CCA AAA TCA ACG GGA CTT TCC AAA ATG TCG TAA CAA CTC CGC CCC ATT5'

540



CAAAT

TATA box

GAC GCA AAT GGG CGG TAG GCG TGT ACG GTG GGA GGT CTA TAT AAG CAG AAC TCT5'

594

T7 promot...ing siteh...r

CTG GCT AAC TAG AGA ACC CAC TGC TTA CTG GCT TAT CGA AAT TAA TAC GAC TCA5'

648

T7 pr... site

BamHIHindIII

CTA TAG GGA GAC CCA AGC TTG GTA CCG AAC TCG GAT CCA CTA GTA ACG GCC GCC5'

702

Ig K-Chain

EcoRI

M E T D T L L

1 2 3 4 5 6 7

AGT GTG CTG GAA TTC GGC TTG GGG ATA TCC ACC ATG GAG ACA GAC ACA CTC CTG5'

756

Ig K-Chain MCS YbeJ5-253

BglII

L W V L L L W V P G S T G D

8 9 10 11 12 13 14 15 16 17 18 19 20 21

R S

1 2

A A

5 6

CTA TGG GTA CTG CTG CTC TGG GTT CCA GGT TCC ACT GGT GAC AGA TCT gcc gca5'

810

YbeJ5-253

BstEII

G S T L D K I A K N G V I V V G H R

7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

ggc agc acg ctg gac aaa atc gcc aaa aac ggt gtg att gtc gtc ggt cac cgt5'

864

YbeJ5-253

E S S V P F S Y Y D N Q Q K V V G Y

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

gaa tct tca gtg cct ttc tct tat tac gac aat cag caa aaa gtg gtg ggt tac5'

918


YbeJ5-253

S Q D Y S N A I V E A V K K K L N K

43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

tcg cag gat tac tcc aac gcc att gtt gaa gca gtg aaa aag aaa ctc aac aaa5'

972

YbeJ5-253

P D L Q V K L I P I T S Q N R I P L

61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78

ccg gac ttg cag gta aaa ctg att ccg att acc tca caa aac cgt att cca ctg5'

1026

YbeJ5-253

L Q N G T F D F E C G S T T N N V E

79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96

ctg caa aac ggc act ttc gat ttt gaa tgt ggt tct acc acc aac aac gtc gaa5'

1080

YbeJ5-253

R Q K Q A A F S D T I F V V G T R L

97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114

cgc caa aaa cag gcg gct ttc tct gac act att ttc gtg gtc ggt acg cgc ctg5'

1134

YbeJ5-253

L T K K G G D I K D F A N L K D K A

115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132

ttg acc aaa aag ggt ggc gat atc aaa gat ttt gcc aac ctg aaa gac aaa gcc5'

1188

YbeJ5-253

V V V T S G T T S E V L L N K L N E

133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150

gta gtc gtc act tcc ggc act acc tct gaa gtt ttg ctc aac aaa ctg aat gaa5'

1242

YbeJ5-253

FspAI

E Q K M N M R I I S A K D H G D S F

151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168

gag caa aaa atg aat atg cgc atc atc agc gcc aaa gat cac ggt gac tct ttc5'

1296


YbeJ5-253

BmgBI

R T L E S G R A V A F M M D D V L L

169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186

cgc acc ctg gaa agc ggt cgt gcc gtt gcc ttt atg atg gat gac GTG ctg ctg5'

1350

YbeJ5-253

A G E R A K A K K P D N W E I V G K

187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204

gcc ggt gaa cgt gcg aaa gcg aag aaa cca gac aac tgg gaa atc gtc ggc aag5'

1404

YbeJ5-253

P Q S Q E A Y G C M L R K D D P Q F

205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222

ccg cag tct cag gag gcc tac ggt tgt atg ttg cgt aaa gat gat ccg cag ttc5'

1458

YbeJ5-253

K K L M D D T I A Q V Q T S G E A E

223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240

aaa aag ctg atg gat gac acc atc gct cag gtg cag acc tcc ggt gaa gcg gaa5'

1512

YbeJ5-253 cpGFP 147-238

K W F D K W F K N P I L V

241 242 243 244 245 246 247 248 249 250 251 252 253

S H N V Y

147 148 149 150 151

aaa tgg ttt gat aag tgg ttc aaa aat cca att ctg gtg AGC CAC AAC GTC TAT5'

1566

cpGFP 147-238

I M A D K Q K N G I K A N F K I R H

152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169

ATC ATG GCC GAC AAG CAG AAG AAC GGC ATC AAG GCG AAC TTC AAG ATC CGC CAC5'

1620

cpGFP 147-238

N I E D G G V Q L A Y H Y Q Q N T P

170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187

AAC ATC GAG GAC GGC GGC GTG CAG CTC GCC TAC CAC TAC CAG CAG AAC ACC CCC5'

1674


cpGFP 147-238

I G D G P V L L P D N H Y L S T Q S

188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205

ATC GGC GAC GGC CCC GTG CTG CTG CCC GAC AAC CAC TAC CTG AGC ACC CAG TCC5'

1728

cpGFP 147-238

K L S K D P N E K R D H M V L L E F

206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223

AAA CTT TCG AAA GAC CCC AAC GAG AAG CGC GAT CAC ATG GTC CTG CTG GAG TTC5'

1782

cpGFP 147-238 Linker

BsrGI

V T A A G I T L G M D E L Y K

224 225 226 227 228 229 230 231 232 233 234 235 236 237 238

G G T

1 2 3

GTG ACC GCC GCC GGG ATC ACT CTC GGC ATG GAC GAG CTG TAC AAG GGC GGT ACC5'

1836

Linker cpGFP 1-146

G G S

4 5 6

M V S K G E E L F T G V V P I

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

GGA GGG AGC ATG GTG AGC AAG GGC GAG GAG CTG TTC ACC GGG GTG GTG CCC ATC5'

1890

cpGFP 1-146

L V E L D G D V N G H K F S V S G E

16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33

CTG GTC GAG CTG GAC GGC GAC GTA AAC GGC CAC AAG TTC AGC GTG TCC GGC GAG5'

1944

cpGFP 1-146

G E G D A T Y G K L T L K F I C T T

34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51

GGC GAG GGC GAT GCC ACC TAC GGC AAG CTG ACC CTG AAG TTC ATC TGC ACC ACC5'

1998

cpGFP 1-146

G K L P V P W P T L V T T L T Y G V

52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69

GGC AAG CTG CCC GTG CCC TGG CCC ACC CTC GTG ACC ACC CTG ACC TAC GGC GTG5'

2052


cpGFP 1-146

Q C F S R Y P D H M K Q H D F F K S

70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87

CAG TGC TTC AGC CGC TAC CCC GAC CAC ATG AAG CAG CAC GAC TTC TTC AAG TCC5'

2106

cpGFP 1-146

A M P E G Y I Q E R T I F F K D D G

88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105

GCC ATG CCC GAA GGC TAC ATC CAG GAG CGC ACC ATC TTC TTC AAG GAC GAC GGC5'

2160

cpGFP 1-146

N Y K T R A E V K F E G D T L V N R

106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123

AAC TAC AAG ACC CGC GCC GAG GTG AAG TTC GAG GGC GAC ACC CTG GTG AAC CGC5'

2214

cpGFP 1-146

I E L K G I D F K E D G N I L G H K

124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141

ATC GAG CTG AAG GGC ATC GAC TTC AAG GAG GAC GGC AAC ATC CTG GGG CAT AAG5'

2268

cpGFP 1-146 YbeJ 254-272

L E Y N F N

142 143 144 145 146 147

N P L N M N F E L S D E

254 255 256 257 258 259 260 261 262 263 264 265

CTG GAG TAC AAC TTT AAC aaC CCc ctg aac atg aat ttc gaa ctg tca gac gaa5'

2322

YbeJ 254-272 YbeJ Æ272-280

AccISalI

PstI

M K A L F K E

266 267 268 269 270 271 272

P N D K A L K L Q V D

1 2 3 4 5 6 7 8 9 10 11

atg aaa gca ctg ttc aaa gaa ccg aat gac aag gca ctg aaa CTG CAG GTC GAC5'

2376

PDGFR 513-561myc_epi

BsmI

E Q K L I S E E D L N

12 13 14 15 16 17 18 19 20 21 22

A V G Q D T Q

513 514 515 516 517 518 519

GAA CAA AAA CTC ATC TCA GAA GAG GAT CTG AAT GCT GTG GGC CAG GAC ACG CAG5'

2430


PDGFR 513-561

E V I V V P H S L P F K V V V I S A

520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537

GAG GTC ATC GTG GTG CCA CAC TCC TTG CCC TTT AAG GTG GTG GTG ATC TCA GCC5'

2484

PDGFR 513-561

XcmI

I L A L V V L T I I S L I I L I M L

538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555

ATC CTG GCC CTG GTG GTG CTC ACC ATC ATC TCC CTT ATC ATC CTC ATC ATG CTT5'

2538

PDGFR 513-561 r.0.401

BmeT110IAvaIXhoI

NotI

W Q K K P R .

556 557 558 559 560 561 562

TGG CAG AAG AAG CCA CGT TAG GCG GCC GCT CGA GAT CAG CCT CGA CTG TGC CTT5'

2592

CTA GTT GCC AGC CAT CTG TTG TTT GCC CCT CCC CCG TGC CTT CCT TGA CCC TGG5'

2646

AAG GTG CCA CTC CCA CTG TCC TTT CCT AAT AAA ATG AGG AAA TTG CAT CGC ATT5'

2700

GTC TGA GTA GGT GTC ATT CTA TTC TGG GGG GTG GGG TGG GGC AGG ACA GCA AGG5'

2754

BbsI

GGG AGG ATT GGG AAG ACA ATA GCA GGC GTG CTG GGG ATG CGG TGG GCT CTA TGG5'

2808

CTT CTG AGG CGG AAA GAA CCA GTG GCG GTA ATA CGG TTA TCC ACA GAA TCA GGG5'

2862

NspIAflIIIPciI

GAT AAC GCA GGA AAG AAC ATG TGA GCA AAA GGC CAG CAA AAG GCC AGG AAC CGT5'

2916

AAA AAG GCC GCG TTG CTG GCG TTT TTC CAT AGG CTC CGC CCC CCT GAC GAG CAT5'

2970


CAC AAA AAT CGA CGC TCA AGT CAG AGG TGG CGA AAC CCG ACA GGA CTA TAA AGA5'

3024

TAC CAG GCG TTT CCC CCT GGA AGC TCC CTC GTG CGC TCT CCT GTT CCG ACC CTG5'

3078

CCG CTT ACC GGA TAC CTG TCC GCC TTT CTC CCT TCG GGA AGC GTG GCG CTT TCT5'

3132

CAT AGC TCA CGC TGT AGG TAT CTC AGT TCG GTG TAG GTC GTT CGC TCC AAG CTG5'

3186

GGC TGT GTG CAC GAA CCC CCC GTT CAG CCC GAC CGC TGC GCC TTA TCC GGT AAC5'

3240

TAT CGT CTT GAG TCC AAC CCG GTA AGA CAC GAC TTA TCG CCA CTG GCA GCA GCC5'

3294

ACT GGT AAC AGG ATT AGC AGA GCG AGG TAT GTA GGC GGT GCT ACA GAG TTC TTG5'

3348

AAG TGG TGG CCT AAC TAC GGC TAC ACT AGA AGG ACA GTA TTT GGT ATC TGC GCT5'

3402

CTG CTG AAG CCA GTT ACC TTC GGA AAA AGA GTT GGT AGC TCT TGA TCC GGC AAA5'

3456

CAA ACC ACC GCT GGT AGC GGT GGT TTT TTT GTT TGC AAG CAG CAG ATT ACG CGC5'

3510

AGA AAA AAA GGA TCT CAA GAA GAT CCT TTG ATC TTT TCT ACG GGG TCT GAC GCT5'

3564

CAG TGG AAC GAA AAC TCA CGT TAA GGG ATT TTG GTC ATG AGA TTA TCA AAA AGG5'

3618

ATC TTC ACC TAG ATC CTT TTA AAT TAA AAA TGA AGT TTT AAA TCA ATC TAA AGT5'

3672

BstAPIEcoO109I

ATA TAT GAG TAA CCT GAG GCT ATG GCA GGG CCT GCC GCC CCG ACG TTG GCT GCG5'

3726

AGC CCT GGG CCT TCA CCC GAA CTT GGG GGG TGG GGT GGG GAA AAG GAA GAA ACG5'

3780

CGG GCG TAT TGG CCC CAA TGG GGT CTC GGT GGG GTA TCG ACA GAG TGC CAG CCC5'

3834

TGG GAC CGA ACC CCG CGT TTA TGA ACA AAC GAC CCA ACA CCG TGC GTT TTA TTC5'

3888

TGT CTT TTT ATT GCC GTC ATA GCG CGG GTT CCT TCC GGT ATT GTC TCC TTC CGT5'

3942


GTT TCA GTT AGC CTC CCC CTA GGG TGG GCG AAG AAC TCC AGC ATG AGA TCC CCG5'

3996

CGC TGG AGG ATC ATC CAG CCG GCG TCC CGG AAA ACG ATT CCG AAG CCC AAC CTT5'

4050

TCA TAG AAG GCG GCG GTG GAA TCG AAA TCT CGT GAT GGC AGG TTG GGC GTC GCT5'

4104

TGG TCG GTC ATT TCG AAC CCC AGA GTC CCG CTC AGA AGA ACT CGT CAA GAA GGC5'

4158

GAT AGA AGG CGA TGC GCT GCG AAT CGG GAG CGG CGA TAC CGT AAA GCA CGA GGA5'

4212

AGC GGT CAG CCC ATT CGC CGC CAA GCT CTT CAG CAA TAT CAC GGG TAG CCA ACG5'

4266

RsrII

CTA TGT CCT GAT AGC GGT CCG CCA CAC CCA GCC GGC CAC AGT CGA TGA ATC CAG5'

4320

AAA AGC GGC CAT TTT CCA CCA TGA TAT TCG GCA AGC AGG CAT CGC CAT GGG TCA5'

4374

CGA CGA GAT CCT CGC CGT CGG GCT TGC TCG CCT TGA GCC TGG CGA ACA GTT CGG5'

4428

BclI

CTG GCG CGA GCC CCT GAT GCT CTT GAT CAT CCT GAT CGA CAA GAC CGG CTT CCA5'

4482

TCC GAG TAC GTG CTC GCT CGA TGC GAT GTT TCG CTT GGT GGT CGA ATG GGC AGG5'

4536

TAG CCG GAT CAA GCG TAT GCA GCC GCC GCA TTG CAT CAG CCA TGA TGG ATA CTT5'

4590

TCT CGG CAG GAG CAA GGT GAG ATG ACA GGA GAT CCT GCC CCG GCA CTT CGC CCA5'

4644

Tth111I

ATA GCA GCC AGT CCC TTC CCG CTT CAG TGA CAA CGT CGA GCA CAG CTG CGC AAG5'

4698

MscI

GAA CGC CCG TCG TGG CCA GCC ACG ATA GCC GCG CTG CCT CGT CTT GCA GTT CAT5'

4752


BbeISfoI

NarI

KasI

TCA GGG CAC CGG ACA GGT CGG TCT TGA CAA AAA GAA CCG GGC GCC CCT GCG CTG5'

4806

ACA GCC GGA ACA CGG CGG CAT CAG AGC AGC CGA TTG TCT GTT GTG CCC AGT CAT5'

4860

AGC CGA ATA GCC TCT CCA CCC AAG CGG CCG GAG AAC CTG CGT GCA ATC CAT CTT5'

4914

ClaIBsaBI

GTT CAA TCA TGC GAA ACG ATC CTC ATC CTG TCT CTT GAT CGA TCT TTG CAA AAG5'

4968

CCT AGG CCT CCA AAA AAG CCT CCT CAC TAC TTC TGG AAT AGC TCA GAG GCC GAG5'

5022

GAG GCG GCC TCG GCC TCT GCA TAA ATA AAA AAA ATT AGT CAG CCA TGG GGC GGA5'

5076

GAA TGG GCG GAA CTG GGC GGA GTT AGG GGC GGG ATG GGC GGA GTT AGG GGC GGG5'

5130

ACT ATG GTT GCT GAC TAA TTG AGA TGC TTG CTT TGC ATA CTT CTG CCT GCT GGG5'

5184

SexAI

GAG CCT GGG GAC TTT CCA CAC CTG GTT GCT GAC TAA TTG AGA TGC TTG CTT TGC5'

5238

ATA CTT CTG CCT GCT GGG GAG CCT GGG GAC TTT CCA CAC CCT AAC TGA CAC ACA5'

5292

TTC CAC AGC TGG TTC TTT CCG CCT CAG GAC TCT TCC TTT TTC AAT AAA TCA ATC5'

5346

AmpR

TAA AGT ATA TAT GAG TAA ACT TGG TCT GAC AGT TAC CAA TGC TTA ATC AGT GAG5'

5400

AmpR

AhdI

GCA CCT ATC TCA GCG ATC TGT CTA TTT CGT TCA TCC ATA GTT GCC TGA CTC CCC5'

5454

AmpR

GTC GTG TAG ATA ACT ACG ATA CGG GAG GGC TTA CCA TCT GGC CCC AGT GCT GCA5'

5508


AmpR

ATG ATA CCG CGA GAC CCA CGC TCA CCG GCT CCA GAT TTA TCA GCA ATA AAC CAG5'

5562

AmpR

CCA GCC GGA AGG GCC GAG CGC AGA AGT GGT CCT GCA ACT TTA TCC GCC TCC ATC5'

5616

AmpR

CAG TCT ATT AAT TGT TGC CGG GAA GCT AGA GTA AGT AGT TCG CCA GTT AAT AGT5'

5670

AmpR

TTG CGC AAC GTT GTT GCC ATT GCT ACA GGC ATC GTG GTG TCA CGC TCG TCG TTT5'

5724

AmpR

GGT ATG GCT TCA TTC AGC TCC GGT TCC CAA CGA TCA AGG CGA GTT ACA TGA TCC5'

5778

AmpR

PvuI

CCC ATG TTG TGC AAA AAA GCG GTT AGC TCC TTC GGT CCT CCG ATC GTT GTC AGA5'

5832

AmpR

AGT AAG TTG GCC GCA GTG TTA TCA CTC ATG GTT ATG GCA GCA CTG CAT AAT TCT5'

5886

AmpR

ScaI

CTT ACT GTC ATG CCA TCC GTA AGA TGC TTT TCT GTG ACT GGT GAG TAC TCA ACC5'

5940

AmpR

AAG TCA TTC TGA GAA TAG TGT ATG CGG CGA CCG AGT TGC TCT TGC CCG GCG TCA5'

5994

AmpR

ATA CGG GAT AAT ACC GCG CCA CAT AGC AGA ACT TTA AAA GTG CTC ATC ATT GGA5'

6048


XmnI

AAA CGT TCT TCG GGG CGA AAA CTC TCA AGG ATC TTA CCG CTG TTG AGA TCC AGT5'

6102

TCG ATG TAA CCC ACT CGT GCA CCC AAC TGA TCT TCA GCA TCT TTT ACT TTC ACC5'

6156

AGC GTT TCT GGG TGA GCA AAA ACA GGA AGG CAA AAT GCC GCA AAA AAG GGA ATA5'

6210

AGG GCG ACA CGG AAA TGT TGA ATA CTC ATA CTC TTC CTT TTT CAA TAT TAT TGA5'

6264

AGC ATT TAT CAG GGT TAT TGT CTC ATG AGC GGA TAC ATA TTT GAA TGT ATT TAG5'

6318

AAA AAT AAA CAA ATA GGG GTT CCG CGC ACA TTT CCC CGA AAA GTG CCA CCT GAC5'

6372

F1 ori

GCG CCC TGT AGC GGC GCA TTA AGC GCG GCG GGT GTG GTG GTT ACG CGC AGC GTG5'

6426

F1 ori

ACC GCT ACA CTT GCC AGC GCC CTA GCG CCC GCT CCT TTC GCT TTC TTC CCT TCC5'

6480

F1 ori

TTT CTC GCC ACG TTC GCC GGC TTT CCC CGT CAA GCT CTA AAT CGG GGG CTC CCT5'

6534

F1 ori

TTA GGG TTC CGA TTT AGT GCT TTA CGG CAC CTC GAC CCC AAA AAA CTT GAT TAG5'

6588

F1 ori

AloI'BsaXI'

DraIII

GGT GAT GGT TCA CGT AGT GGG CCA TCG CCC TGA TAG ACG GTT TTT CGC CCT TTG5'

6642

F1 ori

AloIBsaXI

ACG TTG GAG TCC ACG TTC TTT AAT AGT GGA CTC TTG TTC CAA ACT GGA ACA ACA5'

6696


F1 ori

PsiI

CTC AAC CCT ATC TCG GTC TAT TCT TTT GAT TTA TAA GGG ATT TTG CCG ATT TCG5'

6750

F1 ori

GCC TAT TGG TTA AAA AAT GAG CTG ATT TAA CAA AAA TTT AAC GCG AAT TTT AAC5'

6804

F1 ori

BssHIIMauBI

AAA ATA TTA ACG CTT ACA ATT TAC G5'

6829

Documents

CGC GCG TTG ACA TTG ATT ATT GAC TAG TTA TTA ATA GTA