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Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State College Panhandle Area Educational Consortium 5230 West Highway 98 753 West Boulevard Panama City, Florida 32401 Chipley, Florida 32428 850-769-1551 877-873-7232 www.gulfcoast.edu Biology Partnership (A Teacher Quality Grant)

Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

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Page 1: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Cells – EnergyAerobic and Anaerobic

RespirationJill Hansen

Tammy Stundon

Gulf Coast State College Panhandle Area Educational Consortium

5230 West Highway 98 753 West Boulevard

Panama City, Florida 32401 Chipley, Florida 32428

850-769-1551 877-873-7232

www.gulfcoast.edu

Biology Partnership

(A Teacher Quality Grant)

Page 2: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Pre-test

Q and A Board

Page 3: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Due to limited content and blending with photosynthesis….

Lab Safety

Page 4: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway
Page 5: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

LOCATION OF SAFETY EQUIPMENT IN THIS CLASSROOM

• FIRE EXTINGUISHERS (NEVER USE ON A HUMAN !!!!!)

• EYE WASH

• SHOWER

• GOGGLE CABINET WITH UV LIGHT

• FIRE BLANKET

• SAND AND ABSORBANT SUBSTANCES

• EMERGENCY CALL BUTTON ON PHONE

• EMERGENCY GAS CUTOFF

• EMERGENCY ELECTRIC CUTOFF

• EXHAUST FAN SWITCH

• APRONS

• BROOM AND DUSTPAN

• Fire drill

• Any others? Check your own classroom!

Have the class scavenger hunt with the items listed. As they find the item, mark it on their map and describe it!

Page 6: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

FOOTWEAR—NO OPEN-TOED SHOES

Page 7: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

USE SAFETY GOGGLES

• Kept in a goggle cabinet

• Sterilized before each lab using UV light

– Why?

• Goggles are protective eye wear NOT protective forehead wear!

Page 8: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

GOGGLES PROTECT THE EYES FROM CHEMICALS. SOME CHEMICALS CAN BE CORROSIVE TO THE CORNEA AND CAN

CAUSE PERMANENT EYE DAMAGE. YOU NEED TO BE CAREFUL---- ESPECIALLY IF YOU WEAR CONTACT LENSES . THE CHEMICAL CAN GO UNDER THE CONTACT LENS AND

ATTACK THE EYE SURFACE

Page 9: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

AN EYEWASH CAN BE USED IF A CHEMICAL DOES GET IN YOUR EYE. THE EYE MUST BE RINSED FOR A MINIMUM OF 15

MINUTES WITH MANY CHEMICALS

Page 10: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

SHOWER

• Not for a fire– Why?

• For chemical spills over more than just the arms

Page 11: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

GLOVES SHOULD BE WORN DURING SOME LABS

Which labs do you need gloves?

Are there different types?

Page 12: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

APRONS or lab coats should be worn during some labs

Page 13: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

LONG HAIR

• Hair should be tied back while performing a lab, especially while dealing with open flames or chemicals

Page 14: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

No food or drink in the lab!

Page 15: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Never point a test tube towards another person or towards your face. Why?

Page 16: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

FIRE EXTINGUISHERS

Page 17: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

• A SAFE LAB?

Page 18: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

What’s wrong with this picture?

Page 19: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

LAB SAFETY VIOLATIONS

Page 20: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Test your skillsLab safety Scenario Activity

Reinforce to your class that misconduct and horse playing is NOT tolerated!Safety First

• Why safety comes first!

• Spongebob Lab safety supplement

• Lab Safety by Design exemplars

Page 21: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Aerobic Cellular Respiration

ACR Rap

Page 22: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Florida Next Generation Sunshine State Standards

The Standards (3)

• SC.912.L.18.10 – Connect the role of adenosine triphosphate (ATP) to energy transfers within a cell

• SC. 912.L.18.8 – Identify the reactants, products, and basic functions of the two stages of cellular respiration; aerobic (glycolysis) and aerobic cellular respiration

• SC.912.L.18.6 – Discuss the role of anaerobic respiration in living things and in human society

Also assesses:

SC.912.L.18.7 Identify the reactants, products, and functions of photosynthesis

SC.912.L. 18.9 Explain the interrelated nature of photosynthesis and cellular respiration

Page 23: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Florida Next Generation Sunshine State Standards

Prior knowledge • SC.6.L.14.4 -

• SC.8.L.18.1 Describe and know that all living things are composed of four basic categories of macromolecules and share same basic needs for life

• SC.8.L.18.2 Describe and know that living organisms acquire the energy they need for life processes through various metabolic pathways (primarily photosynthesis and cellular respiration

• SC.7.P.11.2 Investigate and describe the transformation of energy from one form to another

• SC.7.P.11.3 Cite evidence to explain that energy cannot be created nor destroyed, only changed from one form to another

• Chemical reactions in living things follow basic rules of chemistry and are usually regulated by enzymes

• The unique chemical properties of carbon and water make life on Earth possible

Page 24: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Florida Next Generation Sunshine State Standards

Cognitive complexity: Moderate

Benchmark Clarifications: • Students will identify the reactants, products and/or the basic

functions of aerobic and anaerobic cellular respiration• Students will connect the role of adenosine triphosphate (ATP)

to energy transfers within the cell

Content limits: • Items will not require the memorization of stages, specific

events, or intermediate molecules produced during these processes

• Items will not require the balancing of equations

Stimulus attributes: Scenarios may include chemical equations. Scenarios referring to adenosine triphosphate should use the abbreviation ATP rather than the words adenosine triphosphate

Page 25: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Where is energy within a molecule stored?

What are the different forms of energy?

What are the various laws of energy? Carbon molecule needs how

many bonds to be stable?

Page 26: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

CHEMICAL energystored in plants

HEAT

Energy lost in the form of

LIGHT energy from the sun

Recall the First Law of Thermodynamics!!

Page 27: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Carbohydrates: C6H12O6

Glucose – the energy storing molecule for plants, animals use to make ATP

Macromolecules

Nucleic Acids: ATP

Page 28: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

A form of potential energy stored in chemical bonds

CHEMICAL ENERGY

GlucoseC6H12O6

ENERGY

Food is a form of CHEMICAL energy!

Page 29: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

ADENOSINE TRIPHOSPHATE (ATP) AND ADENOSINE DIPHOSPHATE (ADP)

Symbol for ATP used in this book

Symbol for ADP used in this book

The green halo represents ATP’s potential energy.

Ribose (sugar)ENERGY

AdeninePhosphategroups

SeparatephosphategroupPhosphate groups

Ribose (sugar)

Pi

High-energybonds

Adenine

ATP ADP

Page 30: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

ENERGY

Pi

ENERGY

ATP

ADPEnergy released from the breakdown of food

Energy todo work

ATP can be used and recycled hundreds of thousands of times!

Page 31: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Macromolecules continued…Proteins: Enzymes and how they work

How we get nutrients inside the cell and waste out?

Organelles: mitochondria

Page 32: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Sun1. PHOTOSYNETHESISPlants capture energy from the sun and store it in the chemical bonds of sugars and other food molecules.

2. CELLULAR RESPIRATION (Aerobic and Anaerobic) Plants, animals, and fungi release the energy stored in the chemical bonds of food molecules and use it as fuel.

ENERGYAll life depends upon capturing energy from the sun and converting it into a form that living things can use!

Energy is captured/stored in 3 different reactions

What is this leading up to?

Page 33: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

The Circle of LifeRespiration and photosynthesis are dependent

on each other. The products of one are the reactants of another!

Page 34: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

We need to recall that information to understand today’s journey….

Aerobic Cellular Respiration!!!!

Anaerobic Cellular Respiration!!

Page 35: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Dixie Cup Respiration

Page 36: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

5 minutes!!

Page 37: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Respiration

• Two Types of Respiration

• 1. Cellular Respiration – (aerobic)

• -requires O2 and sugar is completely broken down into CO2 ATP, and H2O

• 2. Fermentation – (anaerobic)

• -No O2 is present and sugar is broken down into lactic acid

Page 38: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

CarbondioxideHigh-energy bonds

of food moleculesare broken down, releasing energy.

CELLULAR RESPIRATION

Water

+ ++

ATP

ATP

Water

Oxygen

Oxygen

Sugar

Sugar EnergyCarbon dioxide

INPUT OUTPUT

Page 39: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Showcasing Cellular Respiration • Balloon Respiration Activity

Many teachers take the approach of just doing demonstrations since the concepts can be difficult to understand and/or heavily supplement with worksheets. None of this is wrong! Activities with data sets can reinforce the Science of Nature. Many of the demonstrations are anaerobic!

Nevertheless, remember, application and synthesis are higher order thinking levels!

Your challenge is to make the students think! Set up labs as inquiry or argumentative driven inquiry.

Page 40: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Don’t be overwhelmed with details

• Honda cog

Page 41: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Aerobic Cellular Respiration

To produce ATP - 3 main reactions

I. Glycolysis - occurs in cytoplasma

- reactant is glucose (sugar)

- an enzyme reacts with the sugar breaking it down

- product is taken to step 2

- a by-product of this reaction is 2 ATP molecules

Page 42: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Aerobic Cellular Respiration

II. Kreb cycle - occurs in matrix of mitochondria

- the product from glycolysis become the reactant- enzyme attaches - breaks it down- Main product is H

- By-products CO2 (fate of CO2?) and 2 ATP molecules

Page 43: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Aerobic Cellular Respiration

III. Electron Transport - The H that is left over in the Kreb cycle is taken to the Cristae

- Oxygen bonds with the H forming water and 32 ATP molecules

Page 44: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Respiration

1. Cellular respiration (aerobic)

- 3 steps

- produces 36 ATP molecules and water

2. Fermentation (anaerobic)

- Glycolysis step only

- produces 2 ATP molecules and in animals Lactic acid and in plants lots of carbon dioxide and alcohol

Page 45: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Respiration Manipulative

Now that you have ‘discovered’ respiration and learned about the process… showcase what YOU know!

Page 46: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

10 minutes!!

Page 47: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Alcoholic Fermentation in Yeast Lab

• Effects of sucrose concentrations on the rate of alcoholic fermentation in yeast

Page 48: Cells – Energy Aerobic and Anaerobic Respiration Jill Hansen Tammy Stundon Gulf Coast State CollegePanhandle Area Educational Consortium 5230 West Highway

Follow up

• Q & A • Post Test

More respiration tomorrow as we cover photosynthesis and compare the reactions!