cDNA Clones, Vectors and Gene Synthesis Your complete soultions for molecular biology needs

ORF Clones TrueClone n TrueORF n PrecisionShuttle n TrueORF Gold

Non-protein Coding Clones miRNA plasmids n 3'-UTR reporter plasmids n shRNA plasmids

Gene Synthesis: Clone by Design

Any variant n Any species n Any vector

PO Box 520HA9 7YN, UK

Email: info@insightbio.comWeb: www.insightbio.com

Tel: 0800 073 3133 +44(0)20 8385 0303Fax: 0800 953 0268 +44(0)20 8385 0302

OriGene Clone Offering OverviewComprehensive. Quality. Fast Delivery.WhenyouneedaDNAclone,beitacDNAcloneoracloneofnon-codingsequence,OriGeneisyourbestchoice.

Our100,000ready-to-shipcDNAclonesofferaquickandcost-savingsolution.WiththeacquisitionofBlue

Heron,theleadinggenesynthesiscompany,OriGeneisenhancedwiththeproficiencytocreatecustomizedDNA

clonesofanyspeciesandanydesign.

www.origene.com/cDNA

cDNA clones for protein expression Clones with non-coding sequences

TrueClone

(untagged)

TrueORF

(tagged)

Gene synthesis Gene synthesis miRNA plasmids 3'-UTR reporter

plasmids

Main utility Protein

Expressionin

nativeform

Taggedprotein

expression

Customize Customize miRNAover

expression

Targetvalidation

formiRNA

Expression host Mammalian Mammalian

Shuttleto60

vectors

Customize Customize Mammalian mammalian

Species Human/Mouse Human/Mouse Anyspecies,

anysequence

Anyspecies,

anysequence

Human/mouse Human

HOW TO FIND A CLONE

Search: Asearchboxislocatedatthetopofevery

pageoftheOriGenewebsiteandthefollowingterms

canbeused.

n NCBIAccessionNumber(eg.NM_000044)

n Genesymbolofthegene(eg.VEGF)

n Genename(Caspase8)

n Clonedescription(eg.androgenreceptor,or

kinasedeficientmutant)

Browse: Ontheclonecollectionpage,www.origene.com/

cdna/clone_set.aspx,genescanalsobebrowsedbya

genefamilyorapathway.Afewpopularcategories

arelistedbelowasexamples.

n ProteinKinase n WntPathway

n Phosphotase n Angiogenesis

n GPCR n NotchPathway

n Secreted n TumorMetastasis

Other Providers:Clones arrive as bacterial stock

Day 1Plating for

single colonies

Day 2Inoculate for

bacterial expansion

Day 3Plasmid isolation and verification

Day 4Start your

experiments

OriGene:Clones arrive as

purified plasmid DNA

Start Here Start HereOr

www.origene.com/cDNA

Why Should You Choose OriGene’s cDNA Clones? Quality! Selection! Delivery!

FEATURES

nComprehensive:Genomewidecoverageforhumanandmouseandgenesynthesisavailable

nVersatile:untaggedortaggedclones(>60taggingoptions)

nExpressionvalidation(Seepage6)

nTransfection-readyDNA:Plasmidsarepurifiedwithion-exchangecolumns.

nPathwayFocusedcDNACloneSets(Seepage9)

For protein-coding ORF sequences, there are two type of clones

n TrueClones:Library-based,full-lengthcDNAclonesthatusuallycontainnative5'and3'untranslated

regions;inaCMVmammalianexpressionvector

n TrueORFClones:Taggedopenreadingframe(ORF)clonesinOriGene'sCMVbasedPrecisionShuttle

entryvector.TheORFinsertcanbeeasilyshuttledwithasimpledigestion/ligationreactionintoa

widevarietyoftaggeddestinationvectors.

For non-protein coding sequences, OriGene offers

n miRNAexpressionclone(Seepage10)

n shRNAclones(SeeRNAibrochureorwww.origene.com/RNAi/)

n 3'-UTRreporterclones(Seepage11)

www.origene.com/orf

TrueORF™Myc/DDK- or GFP-tagged ORF clones for convenient application

TheTrueORFproductlineisthenewgenerationofcDNAcloneproducts.UnlikeTrueClones,aTrueORF

cloneenablesexpressionoftheencodedtranscriptasataggedprotein.Thisfacilitatesmultipledownstream

applicationsthatutilizeananti-tagantibodysuchas:Proteindetection,proteinpurification,subcellular

localization,andothers.

TrueORF VECTORS

AllTrueORFinsertsarehousedineitherpCMV6-EntryorpCMV6-AC-GFPvector.Bothvectorsallowforeasy

shuttlingbyasimple'cut-and-paste'mechanismintoanyofthePrecisionShuttleDestinationVectors(seepage

6).ATrueORFcloneexpressestheencodedsequenceasaC-terminallyMycandDDK-taggedproteinoraGFP-

taggedprotein.

DDKisthesameasFLAG.FLAG®isaregisteredtrademarkofSigma-Aldrich.

ThediagramaboveisapplicabletothemajorityofhumanORFsthatdonothaveinternalSgfIandMluIsites.

OtherrarerestrictionsitesintheMCSareutilizedforORFswithinternalsitesforthetwoenzymes.

Ampr

Neor

SV40 ori

ColE1

f1 ori CMV promoter

PolyAsignal

pCMV6-AC-GFP6.6 kb

Sgf IAsc IHind III

Rsr IIMlu INot IXho IPme I

Kozak

GFP

SV40 ori

Neor/Kanr

ColE1

f1 ori CMV promoter

PolyAPolyA

pCMV6-Entry4.9 kb

Sgf IAsc IHind III

Rsr IIMlu INot IXho I

Kozak

DDKMyc

* The last codon before the Stop codon of the ORF

G A T C T G G C A G C A A A T G A T A T C C T G G A T T A C A A G G A T G A C G A C G A T A A G G T T T A A A C G G C C G G C C G CEcoR V DKK-Tag Pme I Fse I

D L A A N D I L D Y K D D D D K V Stop

– – – – – – – – – – – – – – – N N N ACG CGT ACG CGG CCG CTC GAG CAG A A A CTC ATC TCT GA A GAGT R T R R L E Q K L I S E E

Mlu I Not I Xho I Myc.-Tag*

ORF

C T A T A G G G C G G C C G G G A A T T C G T C G A C T G G A T C C G G T A C C G A G G A G A T C T G C C G C C G C G A T C G C C A T G – – – – – –

EcoR I BamH I Kpn I RBS Sgf I

KozacConsensus

G C G A T C G C

Sgf I ORF MIu I

A T G – – – / / – – – N N NC A C G C G T*

www.origene.com/orf

TrueORFS ARE EXCELLENT FORn Mammalianoverexpressionoftaggedproteins

(over60combinationoftagsavailable)

n Detectionoftheexogenouslyintroducedprotein

n Purificationoftheoverexpressedprotein

n Proteininteractionandlocalizationstudies

(e.g.organellemarkers)

n Cellularimagingoftheexogenouslyintroduced

protein

n Taggedproteinexpressioninacell-freesystem

(eg.TNT)

TrueORF ADVANTAGESn Convenience: TrueORFs provide an instant

solution for tagged protein expression

n Flexibility: TrueORF can be shuttled into

multiple destination vectors.

nAccuracy: TrueORFs have verified and

guaranteed insert sequences

n Proven: TrueORFs have been rigorously

tested for expression of the target proteins

and their tags.

Sofar12,000TrueORFhavepassedtheprotein

expressionvalidation.Thenumberisexpectedto

increaseto20,000inthenearfuture.

WesternblotanalysisofHEK293celllysate

over-expressionBLKorBTKtaggedwithwith

indicatedepitopes.

Autophagosome RC10005

Cytoskeleton RC100002

Anti-His Tag(TA 100013)

N-Tag(s) C-Tag(s)Hi

s-HA

-BLK

His-

HA-B

LK

His-

BLK

HA-B

LK

Myc

-Fla

g-BL

K

Myc

-Fla

g-BT

K

Myc

-Fla

g-BT

K

GFP

His-

Flag

-BLK

Anti-HA Tag(TA 100012)

Anti-Myc Tag(TA 100010)

Anti-DDK Tag(TA 100011)

Anti-BTK

Anti-BLK

www.origene.com/orf

PrecisionShuttle SystemTagged protein expression made simple

Toaccommodatediversetaggingneeds,OriGenedevisedthenovelPrecisionShuttle™systemtoalloweasy

subcloningofanORFfromonetaggedvectortoanother.TheTrueORFEntryVectorcontainsC-terminaltagsof

MycandDDK®.AlargepanelofdestinationvectorsareavailablesoyoucanexpressanORFwithdifferenttags

orwithtagsatdifferentendsoftheprotein.ThekeyinthePrecisionShuttlesystemistheutilizationoftworare-

cuttingrestrictionendonucleases,SgfIandMluI.

Sgf I

Sgf I

Sgf I

Sgf I

Sgf I

Protein

TrueORFEntry-Vector

PrecisionShuttle

Destination-Vector

PrecisionShuttle

Destination-Vector

PrecisionShuttle

Destination-Vector

TrueORFEntry-Vector

Digestion with Sgf I and Mlu I

Digestion with Sgf I, Mlu I and CIP

Ligation

Transformation and Selection with Ampicillin

ORF

ProteinORF

ProteinORF

Neor/Kanr

Neor/Kanr

Mlu I

Mlu I

Mlu I

Mlu I

Mlu I

Myc-DDKTag

Tag

Tag

Tag

Neor

Neor

Ampr

Neor

Ampr

Ampr

PRECISIONSHUTTLE VS. RECOMBINATION

SHUTTLE SYSTEM (E.G. GATEWAY® SYSTEM):

GatwayisaregisteredtrademarkofLifeTechnology.

SCHEMATIC OF THE

PRECISION SHUTTLING SYSTEM

PrecisionShuttle™ Gateway®

Functionalentryvector

Nosubcloningneeded

EntryvectorNOTfor

expression

Subcloningneeded

Restriction

enzymes-based

Lowcost

Recombination-based

Expensive

NoIPrestriction Conditionallicensing

Inserts>18Kbare

readilyshuttled

Unstableplasmid

wheninsert>5Kb

2aalinkerappended >10aalinkerappended

www.origene.com/orf

PrecisionShuttle™ VectorsEntry and Destination

PrecisionShuttleEntryVector CellSelection

pCMV6-Entry Neomycin

(C-terminalMycand

DDKTagged,Neomycin-R)

PrecisionShuttleDestinationVector MammilianSelection

pCMV6-AC-His Neomycin

pCMV6-AC-Myc Neomycin

pCMV6-AC-HA Neomycin

pCMV6-AC-DDK Neomycin

pCMV6-AC-Myc-His Neomycin

pCMV6-AC-Myc-DDK Neomycin

pCMV6-AC-HA-His Neomycin

pCMV6-AC-DDK-His Neomycin

pCMV6-AC-GFP Neomycin

pCMV6-AN-His Neomycin

pCMV6-AN-Myc Neomycin

pCMV6-AN-HA Neomycin

pCMV6-AN-DDK Neomycin

pCMV6-AN-His-Myc Neomycin

pCMV6-AN-Myc-DDK Neomycin

pCMV6-AN-His-HA Neomycin

pCMV6-AN-His-DDK Neomycin

pCMV6-AN-GFP Neomycin

pCMV6-AC Neomycin

pTUNEInducible Neomycin

pCMV6-A-BSD Blasticidin

pCMV6-A-EM7-BSD Blasticidin

pCMV6-A-Hygro Hygromycin

pCMV6-A-Puro Puromycin

pCMV6-A-GFP —

pCMV6-AC-IRES-GFP Neomycin

pEX-N-His-GST —

pEX-N-GST —

pEX-N-His —

pEX-C-His —

pEX-1 —

pCMV6-AN-RFP Neomycin

PrecisionShuttleDestinationVector MammilianSelection

pCMV6-AC-RFP Neomycin

pCMV6-AN-YFP Neomycin

pCMV6-AC-YFP Neomycin

pCMV6-AC-FP602 Neomycin

pCMV6-AC-FP635 Neomycin

pCMV6-AC-mKate Neomycin

pCMV6-AC-mGFP Neomycin

pCMV6-AC-mRFP Neomycin

pCMV6-AC-mYFP Neomycin

pCMV6-AC-mBFP Neomycin

pCMV6-AC-mCFP Neomycin

pCMV6-AN-FP602 Neomycin

pCMV6-AN-FP635 Neomycin

pCMV6-AN-mKate Neomycin

pCMV6-AN-mGFP Neomycin

pCMV6-AN-mRFP Neomycin

pCMV6-AN-mYFP Neomycin

pCMV6-AN-mBFP Neomycin

pCMV6-AN-mCFP Neomycin

pCMV6-AC-FC Neomycin

pCMV6-AC-FC-S Neomycin

pCMV6-AN-FC Neomycin

pCMV6-AN-FC-S Neomycin

pCMV6-AC-3DDK Neomycin

pCMV6-AN-3DDK Neomycin

pCMV6-AC-IRES-GFP-Puro Puromycin

pTUNE-GFP Neomycin

pCMV6-AN-GFP-C-His Neomycin

UNDERSTAND A VECTOR BY ITS NAME

p CMV6 - A C - RFP

ExpressionPromoter

Selectionin E. Coli

Terminal Tag(s)

www.origene.com/TrueORF_Gold

HEK293 were transfected with L) empty vector R)

TrueORF for Myc/DDK-tagged hTERT(Cat# RC217436).

The lysates were analyzed using anti-DDK antibody to

show over-expression of hTERT.

TrueORF Gold Clones Expression-validated cDNA Clones

TESTED INDIVIDUALLY BY WESTERN BLOT

EachTrueORFGoldclonehasbeenusedtotransfect

humancelllinesforproteinexpression.OriGenehas

producedover12,000over-expressionlysatesfrom

TrueORFtransfectedHEK293cellsandsubsequently

purifiedover5,000humanproteinsfromthosecells.

SEQUENCE VERIFIED

EachTrueORFGoldcloneisfullysequencedwith

downloadablechromatogramfilespostedonline.

Nomoreworryingaboutmutations,deletions,or

frameshiftwhenusingTrueORFGold.

TRANSFECTION READY

Noneedforsubcloning.Noneedforplasmidpreparation.TrueORFGoldclonesareconstructedwithallelements

(promoter,polyadenylationsignal,drug-selectionmarker,etc)forprotein-expressioninmammaliancells.

EASILY SHUTTLED INTO 60 VECTORS

OriGenehaspreparedover60shuttlevectorswithmatchingcloningsitesforeasytransferoftheinsertusing

PrecisionShuttle,asimplecut-and-pasteprocedure.

n Fluorescent-proteintaggingvectors(27) n Induciblevectors(2)

n Epitope-taggingvectors(23) n Bacterialexpressionvectors(5)

n Selectionmarkers(8)

NEXT-DAY SHIPMENT

158

106

79

48

35

23

www.origene.com/cDNA

TrueClone™Authentic full-length cDNA clones for expression and functional studies of a native protein

OriGene’sTrueClonecoversbothhumanandmousespecies.Thevectorsforthetwospeciesdifferslightly.

TrueCLONES ARE EXCELLENT FOR n Overexpressionofthenativeproteininmammaliancells

n Functionalstudiesofnativeprotein

n TemplatesforquantitativePCR

n Probesforhybridization-baseddetection,suchasNorthernblotsorFISHassays

n Proteinexpressionincell-freesystems(eg.TNT)

TrueCLONE ADVANTAGESn Cost-effectiveandtime-savingalternativetodenovocloning

n Expression-readyandtransfection-ready

n AuthenticcDNAsrepresentingnativetranscripts

n Consistentvectorsystemfacilitateshigh-throughputscreening

All TrueClones are unidirectionally cloned in the EcoR I and Sal I restriction sites.

The Sal I site is destroyed during cloning, and cannot be reused. The insert can be

liberated by a simple digestion with Not I.

All mouse TrueClones are cloned unidirectionally between two sites in the MCS.

Please contact OriGene’s technical support professionals for details.

Amp r

pCMV6-XL4/5/64707 bp

SV40 oriColE1

f1 oriCMV

promoter

Sac I

T7/SP6 Promoter

Not IEcoR IBgl IIKpn IIEcoRVHind IIISal IXba INot ISma I

MCS

PolyAsignal

Kanr/Neor

pCMV6-Kan/Neo4906 bp

SV40 ori

ColE1

f1 ori CMV promoter

T7 Promoter

Sac IEcoR IHind IIISma I

MCS

PolyAsignal

HSV TKPolyA

www.origene.com/cdna/clone_set.aspx

Pathway-focused cDNA Clone Sets

Forresearcherswhowishtoobtainagenefamily-orapathway-focusedcDNAclonecollectionforeither

highthroughputscreeningorforarchiving.OriGeneofferstheeasyoptionofcloneset.Themajorbenefitof

purchasingaclonesetisthemajordiscountdependingonthesizeofthecollection.

PRE-MADE CLONE SETS

Cat# Description Price(subjecttochange)

TCAC105 TransmembraneSet(3,890) $300K

TCAC106 AccessSet10,000(10,000) $250K

TCAC107 AccessProteinKinaseSet(315) $25K

TCAC108 AccessPhosphataseSet(137) $15K

TCAC109 AccessGPCRSet(215) $20K

CLONE SETS FOR CUSTOMIZED ASSEMBLY

OriGene’swebsitelistsover100commonlystudiedpathways,genefamiliesandresearchfocusessothatthe

customercanassembletheirownclonecollectionofinterestwithease.

Samplecollections

Clone Sets TrueORF TrueClone

(Myc-DDK Tagged) (Untagged)

GPCR 269 376

Kinase-deficientmutant N/A 337

ProteinKinase 422 973

Secreted 1087 1259

Transmembrane 3309 4372

Angiogenesis 341 534

Clone Sets TrueORF TrueClone

(Myc-DDK Tagged) (Untagged)

Apoptosis 1498 2315

BreastCancer 319 507

Cytokines 203 241

HumanStemCell 246 339

HumanTumorMetastasis 94 154

Notch 92 144

WntPathway 287 386

Moreonwebsite

www.origene.com/microRNA

MicroRNA Expression PlasmidsComprehensive coverage for human and mouse genome

OriGeneprovidesclonesforover-expressionofmicroRNAofyourchoice.OriGene’sMicroRNAprecursor

containspre-micoRNA(60-70nt)with250-300ntsup-anddown-streamofflankingsequence.Itwasamplified

fromhumangenomicDNAandclonedintoOriGene’spCMV6-MirVector.Upontransfection,thecellular

machinerywillprocesstheCMV-drivenexpressionofmiRNAprecursorintomaturemiRNAandcellularfunction

canbeanalyzed.

FEATURES & BENEFITSn GenomewidemiRNAcoverage—652humanand486mouse

n SequenceconfirmationoftheprecursormicroRNA

n GFPfortransfectionmonitoring

n Neomycinselectionforstablecellestablishment

GFP transfection of microRNA expression plasmids in HEK293 cells

Mir205

Empty Vector Non-transfected

Mir143 Mir34b

miRNA EXPRESSION PLASMIDSSoldindividuallyas10ugtransfection-readyDNAorcanbepurchasedasfollowingsets

CatalogNo. Description

SC410001 MousemiRNAexpressionplasmidset(486vectors,10ugeachin2-Dbarcodedtubes)

SC420001 HumanmiRNAexpressionplasmidset(652vectors,10ugeachin2-Dbarcodedtubes)

SC410002 MousemiRNAexpressionplasmidset(486vectors,2ugeachin96-wellplates)

SC420002 HumanmiRNAexpressionplasmidset(652vectors,2ugeachin96-wellplates)

5’ 3’

3’ 5’ 3’

RISC complex

AAAAAA mGppp

Drosha

Drosha cut

5’ 3’

Dicer Dicer cut

Pri-miRNA

Pre-miRNA

Mature miRNA

www.origene.com/MicroRNA/3-UTR-Clone

3'-UTR Reporter Clones for miRNA Target ValidationLuciferase reporter assays for human genome

The3'-UTRplasmidsprovidequantitativeassessmentoftheinhibitoryeffectbetweenmiRNAsandtheir

potentialtargetgenes.The3'-UTRsequenceofagenewascloneddownstreamofthefireflyluciferasegene.

ThechimerictranscriptlevelisregulatedbyitsinteractionwithmiRNA(s),whichresultsinvariedluciferase

activityquantifiablebyacolorometricassay.MicroRNAsandtheir3'-UTRtargetscanbeassayedfor

endogenousmicroRNAactivityorforoverexpressedmicroRNAinaco-transfectionexperiment.

3'-UTR Target

3'-UTR Target SV40 P

STOP STOP

microRNA RISC complex

AAAAAA

IRES-LucTranslation

NEO IRES Luciferase

Luciferase

INTERACTION

LOW LEVELLUC TRANSLATION

3'-UTR Target

SV40 P

STOP STOP

microRNA RISC complex

Luciferase

AAAAAA

IRES-LucTranslation

NEO IRES Luciferase

NO INTERACTION

LUC TRANSLATION

No interaction between miRNA and UTR.No effect on luciferase expression

Interaction between miRNA and UTR.Reduced luciferase expression

FEATURES & BENEFITSn Genomewidecoverage(>20,000humangene)

n Fireflyluciferaseastheeasy-to-assayreporter

n RFPfortransfectionmonitoring

n HighsensitivityfromIRES-driventranslationof

theexpressioncassette

pCMV - Mir + Mir205 target pCMV - Mir205 + Mir205 target

OriGenehasusedanewdesignadaptedfrom

C.P.Petersenetal.2006,todramaticallyincrease

thesensitivityofdetectionbydecreasingthe3'UTR-

luciferasereporterexpressiontoaverylowlevel.

Luciferase

IRES

EcoR ISgf IAsc I

Rsr IIMlu INot IXba I

PolyA

F1 Ori

SV40

ColE1

CMV

Neor/Kanr

PolyA

RFP

pMirTargetVector7.9 kb

www.origene.com/cdna/organelle_marker.aspx

Organelle Marker PlasmidsLabel subcellular structures with a simple transfection

Cat# Organelle Symbol

RC100004(G)/RC100036(R) Autophagosome ATG12

RC100011(G)/RC100043(R) Autophagosome Di-Ras3

RC100020(G)/RC100052(R) Autophagosome MAP1LC3A

RC100021(G)/RC100053(R) Autophagosome MAP1LC3B

RC100023(G)/RC100055(R) Centrosome PLK1

RC100010(G)/RC100042(R) Coatedpit CLTB

RC100002(G)/RC100034(R) Cytoskeleton ACTB

RC100019(G)/RC100051(R) Cytoskeleton MAP-EB3

RC100022(G)/RC100054(R) Cytoskeleton PFN1

RC100030(G)/RC100062(R) Cytoskeleton TUBA1B

RC100008(G)/RC100040(R) Endoplasmicreticulum CALR

RC100025(G)/RC100057(R) Endosome Rab4

RC100026(G)/RC100058(R) Endosome Rab5

RC100027(G)/RC100059(R) Endosome RhoB

RC100012(G)/RC100044(R) Focaladherinfiber FAK

Cat# Organelle Symbol

RC100031(G)/RC100063(R) Focaladherinfiber VCL

RC100032(G)/RC100064(R) Focaladherinfiber ZYX

RC100005(G)/RC100037(R) Golgiapparatus B4GalT1

RC100029(G)/RC100061(R) Golgiapparatus TGOLN

RC100016(G)/RC100048(R) Lysosome LAMP1

RC100006(G)/RC100038(R) Mitochondria PDHA1

RC100007(G)/RC100039(R) Mitochondria BID

RC100013(G)/RC100045(R) Neuronalaxis GAP43

RC100009(G)/RC100041(R) Nucleus CCND1

RC100018(G)/RC100050(R) Nucleus LMNB1

RC100024(G)/RC100056(R) Peroxisome PXMP2

RC100017(G)/RC100049(R) Plasmamembrane LCK

RC100028(G)/RC100060(R) Synapticvesicles SYP

PRODUCT DESCRIPTION

nGFP-orRFP-taggedhumancDNAclonesfor

organelle-specificmarking

n Individuallyvalidatedbyconfocalmicroscopy

forcorrectlabeling

nEnablelabelingofliveorfixedcellswithout

chemicalorantibodies

APPLICATIONS

nDirectorganellemarking

nMonitoringofproteintrafficking

nStudyorganellemorphologyanddynamics

nProteinco-localizationwithorganelles

nFractiontrackingduringenrichmentorpurification

$480each,providedas10ugpurifiedtransfection-readyplasmids.

LysosomeLAMP1

PeroxisomePXMP2

Golgi ApparatusB4GALT1TGOLN Nucleus

LMNB1CCND1

EndosomeRab4Rab5RhoB

Endoplasmic ReticulumCALR

Focal Adheasion FiberFAKVCLZYX

CentrosomePLK1

MicrotubuleTUBA1B

Membrane ProteinsLCK

MitochondriaBIDPDHA1

Coated PitCLTB Other Cytoskeleton Proteins

MAP-EB3PFN1

Neuronal MarkersGAP43SYP

Actin Stress FibersACTB

AutophagosomeMAP1LC3AMAP1LC3BDiRas3ATG12

: RFP-tagged marker: GFP-tagged marker

Gene Synthesis from Blue Heron

OriGeneacquiredBlueHeron,thegenesynthesisexpert,tobeabletooffera

completesolutionforgeneresearch.

BlueHeronhasbeenthegoldstandardingenesynthesissince1999.Itssuperiortechnologyandoutstanding

qualityaredemonstratedinaseriesofinnovativeachievements.

nThefirstcompanytosynthesizea52KBgene(2007)

nTheprimarysupplierforthe1stbacterialgenome(2008)

nThesoleDNAsourceforthe1stsyntheticcellbyJ.CraigVenterInst.(2010)

WithBlueHeron'scapabilities,OriGenebecomesaone-stopshopforallofyourcDNAneeds.Ourcombined

strengthsprovidesourcustomerswithbroadercoverage,quickerdeliveryandbetterservice.Weare

100%committedtoservingyouwithhighqualitystandardsandimprovedbusinessefficiencyunderstrict

confidentiality.

WHY TRUST BLUE HERON WITH YOUR GENE SYNTHESIS PROJECT?

nGet Any Gene

Ourproprietarytechnologycansynthesizemorechallenginggenesthananyothervendor.

nFast

Mostgenesaredeliveredwithin2-4weeks.

nGuaranteed Quality

Eachgenewillmatchtheexactsequenceyouorder.

nSecure and Confidential

Orderandcommunicateviaasecure,dedicatedwebsite.

nNo Claim to Your Inventions

YouownfullrightandtitletotheproductsdeliveredandretainIPrights.

nOptional Sequence Design

FreecodonoptimizationtoconvertAAsequenceintoDNAsequenceforbestproteinexpression.

www.blueheronbio.com

Optionalcodon optimization

GENEMAKER™

Submit sequencevia secure website

Gene synthesis

Cloning

Sequenceverification

Final DNA product

WWW

T A GA C GA T T

T A GA C GA T T

APPLICATIONS

nProteins

•ProteinExpression

•CodonoptimizedDNAforimproved

proteinexpression

•Recombinantantibodies

•Functionalpeptides

•Proteinmutagenesis

•Mutationstostudyproteinfolding

•ProteinPurificationService

n Immunology

•Recombinantantibodies

•Humanizingantibodies

nGene variants & variant libraries

•Pointmutations,SNPs

•Comprehensivecodonsubstitution

libraries

•Mutantlibraries

nCustom Vectors

•Reshuffledvectorsites

•Multi-resistancevector

•Specializedcassettedrop-invectors

•Customdesignedvectors

•PrecisionShuttleSystem

nGenomics

•Functionalgenomics

•Syntheticchromosomes&genomes

•CloneSets&collections

nLimitless possibilities

•Combinationsofnaturally-occurringDNA

andsyntheticconstructs

•Completelynovelsequencesnotavailable

innature

•Completeflexibilityinpointorregional

deletion/additionmutations

•EngineeredDNAsequencesforsynthetic

biologystudies

ORDER AND SYNTHESIS OVERVIEW

nOptional-codonoptimizationofaminoacid

sequencenSubmitDNAsequencenGeneMaker:synthesizes,clonesandsequence

verifiestheordernPurifiedplasmidDNAandbacteriacontaining

theplasmidshippedviaovernightdeliverynSequencedataandtracedatapostedtothe

customer'ssecurewebsite

www.blueheronbio.com

OriGene, Your Partner in Gene Research and Beyond

KEY TECHNOLOGIES AND PRODUCTSn Full-length cDNA clones, ORF clones in expression-ready vectors

n Gene synthesis: research speci�c gene design

n RNAi research reagents: shRNA, siRNA, and miRNA function and detection

n SYBR Green qPCR assays for mRNA and miRNA detection, primer panels

n Recombinant human proteins

n TrueMAB™ monoclonal antibodies

n Luminex multiplex immunoassays

n Cancer tissue biorepository, TMA, RPPA, and TissueScan qPCR arrays

PO Box 520HA9 7YN, UK

Email: info@insightbio.comWeb: www.insightbio.com

Tel: 0800 073 3133 +44(0)20 8385 0303Fax: 0800 953 0268 +44(0)20 8385 0302