Upload
ciel
View
45
Download
0
Embed Size (px)
DESCRIPTION
Biological chemistry. Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO. Crystal engineering. Biomarker identification. Protein crystallography. Molecules. H 2. ethane. sucrose. Molecules. Molecules. Crystal. Crystal. ”Crystal engineering”, we make a supermolecule!. - PowerPoint PPT Presentation
Citation preview
Carl Henrik Gørbitz, Ute KrengelDepartment of Chemistry, UiO
Crystal engineering
Protein crystallography
Biomarker identification
Molecules
H2
ethanesucrose
Molecules
Molecules
Crystal
Crystal
”Crystal engineering”, we make a supermolecule!
Building blocks
nodelinker
Problem 1
Molecules are very small, hard to
put together one by one
Xe-atoms on Ni-surface (Don Eigler, 1989)
Problem 2
As they are so small, we must put together very many before we reach a macroscopic size
A 1 x 1 x 1 mm sugar crystal contains 1,4 · 1018 molecules
Building blocks
Building blocks
Glue
Intermolecular forces
Must be: strong Directional
Two main types used in CE: Hydrogen bonds Metal coordination
MOFs
“Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or three-dimensional structures that can be porous” (from Wikipedia)
MOF-5
Can we do this without metal ions?
Guanidinium derivatives
Fumaric acid complex
CN
N
NH
H
H
H
H
H
+
Guanidinium derivatives
DNA-based systems
DNA-based systems
DNA-based systems
Applications
Construction of polar materials for non-linear optics molecular magnets porous materials for storage molecular sieves sensors molecule traps biological model systems catalysts and much more
Biomarker identification
Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule
Present in small amounts = hard to identify
Biomarker identification
Nano-HPLC-system
Group
Carl Henrik
Fassil
Lianglin
Malgorzata
Lise-Lotte
StevenOla
Protein crystallography
Structure/Function Studies of Structure/Function Studies of Medically Relevant SystemsMedically Relevant Systems
Department of Chemistry
Ute Krengel
University of Oslo
X-ray crystallography
Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)
MethodsMethods
Glycobiological Targets◦Mucins◦Bacterial Toxins◦Bacterial Adhesins◦Anti-tumor Antibodies◦Mushroom Lectins
Enzymes◦Chorismate mutases
GlycoNor
TargetsTargetsProtStru
ct
Glycobiological Targets:◦Bacterial Toxins – Structure and Function
Investigation of blood group dependence Delivery mechanisms? Drug design
◦Anti-tumor Antibodies for Immunotherapy Recombinant production, crystallization and X-ray
structure determination
Masters ProjectsMasters Projects
GroupGroup
Ute
Øyvind
Hedda
DanielJulie
Dipankar
Dani