Upload
caryn-acosta
View
26
Download
0
Tags:
Embed Size (px)
DESCRIPTION
Bioinformatics 2.0/3.0. Kei Cheung Yale Center for Medical Informatics. Outline. Introduction Web 2.0 Web 3.0 Semantic Web Topic Map Merging Web 2.0 and Web 3.0. Introduction. - PowerPoint PPT Presentation
Citation preview
Bioinformatics 2.0/3.0
Kei Cheung
Yale Center for Medical Informatics
Outline
• Introduction
• Web 2.0
• Web 3.0 – Semantic Web– Topic Map
• Merging Web 2.0 and Web 3.0
Introduction
• The Human Genome Project (HGP) has transformed genome sciences from being experimental to being increasingly computational
• HGP has intensified the growth of bioinformatics• The Web has become a popular medium for accessing
information over the Internet• Numerous bioinformatics databases and tools are Web
accessible• These databases and tools as well as the Web have
become indispensable for modern-day genomic research• Web 1.0 -> Web 2.0 -> Web 3.0
Web 1.0
• It is read-only
• It is about a single person, organization, …
• It is document centric
• It is based on HTML
• It is for human to read
Web 2.0
Web 2.0
• Social networking (wiki, blog, tagging, bookmarking, rating, etc)
• Multimedia content (photo, audio, video, etc)
• Interactive, responsive, and dynamic web interface (Facebook, Flickr, YouTube, etc)
• Mashup (assembly tools and visualization tools)
Folksonomy (Social Tagging)
• Folksonomy is the practice and method of collaboratively creating and managing tags to annotate and categorize content
• In contrast to traditional subject indexing, metadata is not only generated by experts but also by creators and consumers of the content
• Freely chosen keywords are used instead of a controlled vocabulary
Tag Cloud
• A tag cloud (or weighted list in visual design) is a visual depiction of user-generated tags used typically to describe the content of web sites.
Web 2.0 (cont’d)
• It is decentralized
• It is a community/collaborator model instead of authority/consumer model
• It is fun
• It can be seriously used to share and integrate scientific datasets and algorithms
Bioinformatics Applications of Web 2.0
Wiki Proteins
Nature Precedings (pre-publication research and preliminary findings)
Scientific Podcasts
Multimedia (cont’d)
Journal of Visualized Experiments
myExperiment
Mashup (1): Assembly Tools
• Dapper (scrape web content and convert it into machine readable format)
• Yahoo! Pipes (fetch, filter, and integrate data)
Yahoo! Pipes Demo
Yahoo! Pipes Use Case
GeoCommons: Mashup of Maps
Mashup (2): Visualization Tools
• E.g., Google Earth
Geo-Mashup: Google Earth (tracking H5N1 virus over time)
Bioinformatics Mashup’s
• Mashup of biological entities of the same type– Protein network mashup– Sequence annotation mashup
• Mashup of biological entities of different types
Mashup of pathway data and gene expression data
Calvin cycle pathway associated with gene expressions
Challenges to Data Mashup
• Lack of annotation
• Lack of links
• Lack of link semantics
• Lack of data semantics
• Lack of standards or use of standards
Lack of Semantic Annotation
Kei Tsi Daniel Cheng(this is not me!!)
Kei Cheung (16 years ago)
Kei Cheung(6 months ago)
Lack of Links
colllaborators
Lack of Link Semantics
(?)prototyped
Lack of Data Semantics
<html”<body> …<table><tr><td>Alcohol Dehydrogenase 1B (class I), beta polypeptide</td><td>ADH1B</td></tr> …</table> …</body></html>
Lack of Standards (Use of Standards)
• Different naming rules (based on phenotype, sequence, function, organisms, etc)– Armadillo (fruitflies) vs. i-catenin (mice)– PSM1 (human) = PSM2 (yeast); PSM1 (yeast) = PSM2 (human)– Sonic Hedgehog
• ID proliferation – Different ID schemes: 1OF1 (PDB ID) and P06478 (SwissProt
ID) correspond to Herpes Thymidine Kinase– Lexcial variation: GO1234, GO:1234, GO-1234
• Synonyms vs. homonyms– Dopamine receptor D2: DRD2, DRD-2, D2– PSA: prostate specific antigen, puromycin-sensitive
aminopeptidase, psoriatric arthritis, pig serum albumin
Web 3.0
Web 3.0
• It refers to a third generation of Internet-based services that emphasize machine-facilitated understanding of information in order to provide a more productive and intuitive user experience. – Semantic Web– Topic Map
Semantic Web• "The Semantic Web is an extension of the current web in which
information is given well-defined meaning, better enabling computers and people to work in cooperation." -- Tim Berners-Lee, James Hendler, Ora Lassila, The Semantic Web, Scientific American, May 2001
• It provides a common framework that allows data to be shared and reused across application, enterprise, and community boundaries
• It is based on the Resource Description Framework (RDF)– URI for naming/identify web objects– Graph structure (directed acyclic graph or DAG) for connecting web
objects
Resource Description Framework (RDF)
• It is a standard data model (directed acyclic graph) for representing information (metadata) about resources in the World Wide Web
• In general, it can be used to represent information about “things” or “resources” that can be identified (using URI’s) on the Web
• It is intended to provide a simple way to make statements (descriptions) about Web resources
RDF Statement
A RDF statement consists of:• Subject: resource identified by a URI• Predicate: property (as defined in a name space identified by a
URI) • Object: property value (literal) or a resource
A resource can be described by multiple statements.
<?xml version="1.0"?> <rdf:RDF xmlns:rdf=“http://www.w3.org/1999/02/22-rdf-syntax-ns#” xmlns:en=“http://en.wikipedia.org/wiki/” ><rdf:Description about=“http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&list_uids=125”>
<en:name>Alcohol Dehydrogenase 1B (class I), beta polypeptide”></en:name><en:synonym>ADH1B</en:synonym>
</rdf:Description></rdf:RDF>
Graphical & XML Representationhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&list_uids=125
“Alcohol Dehydrogenase 1B (class I), beta polypeptide”
http://en.wikipedia.org/wiki/Namehttp://en.wikipedia.org/wiki/Snynonym
“ADH1B”
RDF Schema (RDFS)
• RDF Schema terms:– Class– Property– type– subClassOf– range– Domain
• Example:<DNASequence, type, Class><Promoter,subClassOf,DNASequence><Protein,type,Class><TranscriptionFactor,subClassOf,Protein><Bind,type,Property><Bind,domain, TranscriptionFactor><Bind,range, Promoter>
Ontologies
• In both computer science and information science, an ontology is a representation of a set of concepts within a domain and the relationships between those concepts.
• It is a shared conceptualization of a domain
• Ontologies are commonly encoded using ontology languages.
Web Ontology Language (OWL)
• Latest standard in ontology languages from the W3C
• Built on top of RDF
• OWL semantically extends RDF while it is syntactically the same as RDF
• Three species of OWL– OWL-Lite– OWL-DL– OWL-Full
OWL > RDF/RDFS
• Cardinality restrictions: (e.g., a gene may have more than one transcription factor binding sites)
• Disjointedness of classes: (e.g., mRNA may be classified either as introns or exons)
• Other OWL constructs – uniqueness: (e.g.,a GO term can have only one GO identifier)– unionOf: (e.g., gene may be the unionOf intron and exons– sameAs: specifying synonymous relationship between classes
(e.g., “Cerebellar Purkinje Cell” sameAs “Purkinje Neuron”).
Topic Map• A topic map (an ISO standard) is used represent
information using topics (concepts), associations, and occurrences
• It is used to organize information in a way that can be optimized for navigation.
association
occurrence
Neuroscience Topic Map
Topic Map Encoding/Querying
• XML Topic Map (XTM)
• Top Map Query Language (TMQL)
Visual Topic Maps
• A Visual Topic Map can be defined as a topic map including visual topics. A visual topic is defined by a topic name which refers to a visual content.
NCBI Site Map
Mosaic of Chinese Characters in Stories about the Meaning of Ideograms
Visualization of the del.icio.us Tags in an Interactive Graph
Combining Semantic Web and Topic Map
Topic MapSemantic Web
Visualization
Machinereasoning
Knowledge organization & representation (mapping between XTM and RDF/OWL)
Web 2.0 Meets Web 3.0
• Folksonomy meets ontology– Tags can evolve into standard heavy-weight
ontologies, while light-weight ontologies can be applied to tagging
• Human readability meets machine readability– Visual network vs. semantic network
• Social network meets semantic network– FOAF, semantic wiki
• Syntactic mashup meets semantic mashup– Dapper and yahoo pipes may become ontologically
aware
Conclusions
• Web 2.0 and 3.0 provides a platform for data/tool sharing and integration (mashup) and scientific collaboration
• More use cases are needed• Question?
– While Web 1.0 has played an important role in organizing/disseminating information produced by HGP, can Web 2.0/3.0 offer more to present “big science” projects like ENCODE?
The End