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.
( ) PH PH .
. (( )).
..- - .
.
. .
Purification Of Enzymes : 1- Molecular Precipitation 2- Adsorption .3- Polymerization4- Centrifugation.5- Electrophoresis .-
IU : 1 30 .
:
. . :. . . ( )
( ) Cofactor . (CO- enzymes) . B.
. Coenzyme Prosthetic group . Apoenzyme Holoenzyme .
:
:
, ACTIVE SITE ( ) Substrate . , , .
Specificity of enzymes : (Absolute specificity) ( (Urease Low Specificity Lipase .Stereo-chemical Specificity
- - .- .- .- .- (pro Enzymes) .- PH ( ) .- ( ) .- ( ) .
:
. (( )) "ase " ( ) . (( ))"ase " (( )) .
1961 "ase " a Systematic Code number for each enzyme [ E . C] 4 .
(3.1.1.3( {3} (3) {1} (1) (1.3) {1} ( ) (3.1.1) {3} 3.1.1.3.
6 : 1961 .
_1 Oxidoreductases . : . . . .
) Phenol-oxidase: Phenol-oxidase (o-Diphenoloxidase:EC1.10.3.1) (p- Diphenoloxidase : EC 1.10.3.2) . Quinone Melanin
) (Catalase ):
Catalase (EC 1. 11.1.6) (H2O2) : 2 H2O2 2 H2O + O2 Catalase
) Peroxidase : Peroxidase (EC 1.11.1.7) (H2O2) : Peroxidase AH2 + H2O2 A + 2H2O
_2 Transferases . . (EC 2.4.1.1) Phosphorylase Dextrins ( ) .
3- (Hydrolases) . () (EC 3.2.1.26) Invertase (Glucose 1-2 Fructose) ( ) ( ) :
3 _ ( ) Hydrolases .
4- Lyases ( ) (Aldolase) 16
5- Isomerases .
6- Ligases (ATP)
. _
. ( -20 ) .
(PH )- PH .
5- Inhibitors (Competitive inhibitor) . .
2. (Non Competitive Inhibitor) . .