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Jan. 2015 LABORATORY WASTE MANAGEMENT Virginia Tech Environmental Health & Safety
Type of Waste: Examples: Collec
t In: Containers Provided by: Disposal Steps:
SOLID BIOWASTE
Biosafety Level 1
gloves paper towels bacteria/fungi on solid media
tissue culture plates
vials, tubes NO LIQUIDS NO CHEMICALS
Clear autoclave bags (NO symbol)
Bags must be inside “Solid Biowaste
BSL1” lidded cans.
Bags: PI labWaste cans: EHS can supply an initial receptacle on a limited basis; PI lab supplies additional receptacles.
Nalgene pans: PI lab or department
1. Autoclave in Nalgene pan using
designated WASTE cycle. 2. Disposal: Reg. Trash WITH BLACK LINER
or RMW.
SOLID BIOWASTEBiosafety Level
2
gloves paper towels bacteria/fungi on solid media
tissue culture plates
vials, tubes NO LIQUIDS NO CHEMICALS
Orange autoclave bags WITH biohazardsymbol
Bags must be inside red “Solid Biowaste BSL2” lidded cans.
Bags: PI labWaste cans: EHS can supply an initial receptacle on a limited basis; PI lab supplies additional receptacles.
Nalgene pans: PI lab or department
1. Autoclave in Nalgene pan using designated WASTE cycle.
2. Dispose in Regulated Medical Waste (RMW).
3. To schedule RMW pickup by EHS, go to: http://www.ehss.vt.edu/programs/waste_regulated_medical.php
LIQUID BIOWASTEBiosafety Level
1 Biosafety Level 2
used tissue culture media
broth for bacterial culture
biological fluids
bio-liquid extracts
bio-supernatants
NO CHEMICALS
Glass or autoclavable plastic
containers with vented
closures
Containers: PI lab
Nalgene pans: PI lab or department
1. Autoclave in Nalgene pan on LIQUID cycle, OR: Add undiluted household bleach to 1:5
final vol/vol concentration & wait 20 min. OR Add other appropriate disinfectant to proper vol/vol concentration.
2. Pour down drain after time for disinfection.
CONTAMINATED SHARPS
Biosafety Level 1
Biosafety Level 2
pipette tips wood sticks needles,
syringes scalpels razor blades glass slides cover slips NO LIQUIDS NO
CHEMICALS
Red Sharps containers with lids (several sizes available)
EHS; use online request system for Sharps & RMW supplies.
1. Autoclave on solid WASTE cycle.2. Dispose in Regulated Medical Waste.3. To schedule RMW pickup by EHS, go to:
http://www.ehss.vt.edu/programs/waste_regulated_medical.php
WHEN WASTE HAS BOTH CHEMICAL & BIOLOGICAL COMPONENTS, CONTACT E.H.S. FOR ASSISTANCE: chemwaste@vt.edu
GLASS WASTEContaminated
beakers, flasks glass pipettes bottles, tubes
Sharps containers in appropriate waste stream
Sharps containers: EHS Contact EHS if you have further questions:chemwaste@vt.edu
SOLID WASTE Contaminated
with Hazardous Chemicals(including
nanoparticles)
gloves paper towels bleach-soaked
items vials, tubes NO LIQUIDS NO BIOWASTE
Blue bags
Bags should be inside “Chemical Hazardous Waste” lidded cans
Bags: EHS
L idded cans : EHS
Pans (secondary containers): PI lab
1.Fill out the online Chemical Waste Pickup form through The EHS Safety Management System for research laboratories, or by using this link:
https://secure.hosting.vt.edu/www.ehss.vt.edu/programs/LMS/waste_management.php
2. Print out a copy of this form & attach it to
waste. 3. Store waste in safe place in appropriate secondary container until pickup. Allow
2 weeks for pickup.
Questions? Contact EHS at chemwaste@vt.edu
SHARPS Chemically
Contaminated(including
nanoparticles)
syringes/needles
blades/scalpels pipette tips glass
slides/slips glass pipettes NO LIQUIDS NO BIOWASTE
Labeled, clear rigid jars + lids
DO NOT COLLECT SHARPS ON THIS LIST IN BLUE BAGS DUE TO PUNCTURE POTENTIAL
Small clear jars suitable for bench: EHS
pipette tips serol. pipettes NO SHARPS
FROM ABOVE LIST
NO LIQUIDS NO BIOWASTE
Clear jars (as above) for small volumes of tips
Blue bags inside lidded can for large volumes of tips & serological pipettes
Bags : EHS
Lidded cans: EHS
LIQUID WASTE Contaminated
with Hazardous Chemicals(including
nanoparticles)
ethidium bromidesolutions
compatible solvents
stains NO BIOWASTE
Leak-proof, lidded containers:-- 5 gal carboys-- Empty solvent bottles*
with original label defaced; relabel ‘Haz. Waste’ & include specific contents + proper hazard warning. No need to rinse if dry.
Keep lids on liquid waste containers.
5 GAL carboys: EHSRecycled bottles: PI lab1. Use empty chemical
bottles for collecting like-waste.
2. Mixed liq. waste : Use empty solvent bottles*--acetonitrile, methanol, isopropanol, acetone, ethanol, hexane, cyclohexane, chloroform
EMPTY CHEMICAL
CONTAINERS
Empty metal cans
Empty plastic/glass bottles
CONTAINERS OF ACCUTELY HAZ. WASTE (“P LIST” CHEMICALS, STRONG OXIDIZERS, AGGRESSIVE ACIDS/BASES, ETC.) --- SUBMIT A HAZ. WASTE FORM FOR EHS PICKUP. Contact EHS if you have further questions:
chemwaste@vt.eduContainers of less hazardous chemicals --- deface labels, place in cardboard carton, close & tape carton securely; take taped carton to dumpster for disposal.
GLASS WASTEUncontaminated
UNCONTAMINATED Broken glass ONLY
NO LIQUID CONTENTS
Glass disposal box
Line box with CLEAR plastic bag
Glass Disposal Boxes & plastic bag liners: PI lab
GLASSWARE DISPOSED OF IN THIS STREAM CANNOT BE CONTAMINATED WITH HAZARDOUS MATERIAL-- No radioactive (any amount), no biological (any amount), no chemical (more than a trace amount—if unsure, discard in Chem Sharps waste).
When box is full, close & tape securely. Take taped box to dumpster for disposal.
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