Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17....

Transcription

Tuesday, November 14, 17

OverviewDNA

Protein

OverviewDNA

Protein

Transcription

Translation

Don’t Write This Down . . .

If the cell wants to make a specific protein it will take a ‘photocopy’ of the section of DNA that carries the information for specific protein

This photocopy is known a RNA

DNA RNA

deoxyribose, phosphate, nucleotide base

ribose, phosphate, nucteotide base

G, C, A, T G, C, A, U (Uracil)

double stranded single stranded

stable unstable

in the nucleus everywhere

Types of RNA

mRNA: Messenger RNA (transcription)tRNA: Transfer RNA (translation)rRNA: Ribosomal RNA (translation)

Stages of Transcription

1) Initiation2) Elongation3) Termination

Initiation

OverviewRNA polymerase binds to a promoter region

Initiation

A promoter is a DNA sequence that is high in adenine and thymine (TATA box)It is adjacent to the gene to be transcribed

Initiation

A promoter acts as a recognition/binding site for the TATA binding protein.

This protein is recognized by RNA polymerase II which docks to DNA

Initiation

RNA polymerase unwinds the DNA and the template strand is exposed.

Only on strand of the DNA is transcribedThe promoter is NOT transcribed

Elongation

RNA polymerase binds with the promoter and builds in a 5’ → 3’ direction

Elongation

No Okasaki fragments are created because only one strand is transcribed (no lagging strand)

Elongation

RNA polymerase works faster than DNA polymerase because there is no proof reading.

Elongation

Uracil complements adenine

Termination

A stop codon signals RNA polymerase to end transcription

UAA, UAG, UGA

Termination

mRNA and RNA polymerase are releasedDNA that was being transcribed rewinds into double-helical form

Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17....

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