NJ AWWA Annual Conference

FORESIGHT AND PREVENTION

THROUGH

FLUID IMAGING

AND

PARTICLE ANALYSIS

Algae at the

Root of

Everyday Issues

Taste and Odor Shorter Filter Runs

Manganese Spikes

Can Careful Monitoring ofAlgal Population Dynamics Lead To Better Water Treatment Solutions?

Forecasted Regulations and

Cyanotoxins

Common Cyanobacteria in Your Water Resource

Microcystis Aphanizomenon Anabaena

Neurotoxins

Algal Blooms

Australia

Taken From: US Chapter on HABs in German FEA Book

www.whoi.edu

Distribution of Harmful Algae Blooms (HABs) in the U.S.

Messages Hidden in

the Algae?

Cyanotoxins?

Microcystis blooms Aphanizomenon Blooms Anabaena Blooms

Short Filter Runs?

Resilient Crustecea Aphanizomenon Blooms Anabaena Blooms

Manganese Spikes?

Columnar Variation Rapid Die Off Population Dynamics

Testing Your Technology?

Sound Frequency Solarbees Consultants Chlorophyll-A, Phycocyanins

EXPENSIVE CHEMICAL

AND

PHYSICAL MONITORING

Chemical Analysis

Anatoxin $300/Test

Saxitoxin $200/Test

Cylindrospermosin $200/Test

Microcystin $150/Test

Consultant Fees

Algae Monitoring $600/Week

Copper Sulfate $2/KG

Cutrine $10/Liter

www.astutelabs.net

www.anuaenv.ie

Traditional Light Microscopy

Time Consuming Procedure

Vague Approximations

Extreme Human Factor

Unassisted Morphology

One and Done

FlowCAM® Imaging System

3 Samples Run in Under 10 Min.

Internal Cytometer

Limiting Human Error

32 Digital Parameters Assist Morphology

Digital Memory Last a Long Time

Elevating Technology

“With the automated instrumentation, a single technician can do in minuteswhat used to take days with a microscope. And we can be more confidentin the data when taking action”

Betsy Reilly, MWRA

THE FLOWCAM® IMAGING SYSTEM

Technical Specs

• Fully self-contained (computer, monitor, etc. included)

• Four magnifications (2X, 4X, 10X and 20X) available to accommodate broad range of sample particle sizes (2µm to 2mm)

• Automatic Classification from Preset Filters

• Intuitive VisualSpreadsheet® software for data capture and analysis

• Many options available including cross-polarized illumination, 488 or 532nm laser, variable emission filters, color camera and many different flow cell sizes

• Processing Capability –• Flow – .1 ml/min to 12 ml/min• Concentration – 5,000,000 particles/ml

Quick Image Acquisition

http://www.youtube.com/watch?v=STp2r5aotfk&feature=youtu.be

SOME OF THE 30+ FLOWCAM IMAGE PARAMETERS

Morphological Parameters

• Length• Width• ESD• ABD• Aspect Ratio• Elongation• Perimeter• Particles per Chain• Relative Chlorophyll • Relative Phycoerythrin/Other• Scatter Signal Value

Image Parameters

• Transparency• Intensity• Sigma Intensity• Compactness• Roughness• Edge Gradient• Average Red• Average Green• Average Blue • Red/Green Ratio• Blue/Green Ratio• Red Blue Ratio

FLUID IMAGING TECHNOLOGIES

WATER TREATMENT GRANT PROGRAM

Grant Recipient

N.J. American WaterCanoe Brook Treatment FacilityShort Hills, New Jersey

Supervising Agent

Scott BrezinskiWater Quality SupervisorPassaic Operating Area

Project Intern

Michael CohrsPhysics Graduate StudentMontclair State University

Evaluate an Optimal Equipment Setup

Develop and Publish an Effective SOP

Collect, Analyze, and Interpret Effective Data

Publish and Present Results

FACTORS UNDER STUDY

Water Treatment Issues

-Manganese Spikes-Short Filter Runs-Cyanobacteria Monitoring-Technology Testing

Standard Operating Procedure-Sampling Procedure-Sample Processing-Sample Analysis-Classification Setup

Equipment Setup-Magnification/Flow Cell Configuration-Frames/Second vs Flow Speed-Data Captured

SAMPLE ACQUISITION

Van Dorn Sampler

Sample Locations: Intake Dock, Reservoir 1

Intake ValveBottom - 270 cmMiddle - 150 cmTop - 20 cm

Number of Samples

4 Depths3 Samples Each DepthCollected Twice a DayTotal: 24 Samples/Day

June July August September October

1 AP 9 AP 24

2 AP 24 AP 24

3 NAPS 20 AP 24 AP - 10x 24

4 AP 24 AP 12

5 AP 24 AP 24

6 AP 24 AP 24

7 AP 24

8 NAPS 20 AP 24

9 AP 24 AP 24

10 NAPS 20 AP 24 Noon - 10x 6 * Van Dorn Start 7/17 & PPML excel

11 AP 24 ** Start of Crustecea 7/30

12 NAPS 20 AP 24 AP 24 *** FC 200 Start 8/21

13 AP 24 ****AP 24 ****Manganese Start

14 AP 24

15 NAPS 20 AP 24 NAPS = NOON/AM/PM/Surface

16 AP 24 AP 24 AP = AM/PM

17 * AP 24 AP 24

18 AP 24

19 AP 24 AP 24

20 AP 24 AP 24

21 *** AP 24

22 AP 24

23 Bill AP 24 AP 24

24 N-A-P-S 3 AP-10x 24 AP 24

25 AP 24 AP 24

26 NAPS 20 AP 24 AP 24

27 AP 24

28 NAPS 20 AP 24

29 AP 24 AP 24

30**AP-10x-

4x/100 24 AP 24

31 AP 24 Total

Total Files 43 268 489 432 90 1322

Total Days 3 13 21 18 5

Sample Schedule 2013

HARDWARE CONFIGURATIONS

4x/300 µm Flow Cell

Too Many Blurry Images

Highest Efficiency – 33%

Little to No Clogging

Somewhat Unreliable

4x/200 µm Flow Cell

Highest Efficiency – 73%

Basically Field of View

Little Clogging

Effective for Quantification

10x/100 µm Flow Cell

Highest Efficiency – 22%

Strong Images

Clogging Disrupts Counts

Great for Identification

ALGAE CLASSIFICATION AND QUANTIFICATION

Auto-Classification

Classification Window for Quick and Automatic Classification

Export Count Summaries to

Excel Worksheet

Build Filters for Specific

Parameters of Interest

Build Your Own Image Libraries

Isolate Selected Images from Multiple Samples Into One Easy to Access Database

Use Libraries to Create Filters for

Simple Classification

Easy Sort and Data Recording

Sorting Using Preset Filter Unique to Your Images

Sort and Filter Through over 32

Digital Parameters

Easy Sorting by Statistical Algorithms

RESULTS AND CONCLUSIONS

Created the SOPs

Optimized the Equipment Setup

Unraveled the Classification System

Began the Development of Image Libraries

Uncovered a Few Possible Treatment

Indicators

Cyanobacteria Monitoring

Manganese Spikes

Filter Clogging Particles

Technology Verification

CYANOBACTERIA MONITORING

Accurate Population Dynamics So You Know When to Test and Treat

Save Money on Cyanotoxin Tests

Treat Only When Necessary

Taste and Odor Issues Controlled

Microcystis Aphanizomenon Anabaena

0

500

1000

1500

2000

2500

Anabaena

Aphanizomenon

Microcystis

MANGANESE SPIKES

0

500

1000

1500

2000

2500

Intake

270 cm

150 cm

20 cm

0

0.1

0.2

0.3

0.4

Manganese

Particles Per mL

0

500

1000

1500

2000

2500

Intake

270 cm

150 cm

20 cm

0

0.1

0.2

0.3

0.4

0.5

Manganese

Particles Per mL

Columnar Variation

Rapid Die Off

FILTER CLOGGING PARTICLES

The Usual Suspects

Public Enemy Number One

Surviving the Gauntlet

Potassium Permanganate

Ozone

Sulfuric Acid

Aluminum Sulfate

Cationic Polymer

AphanizomenonAnabaena

TECHNOLOGY VERIFICATION

Sound Wave Frequency Population Control

Solar Bee Mixers

Photosynthetic Algae Quantification

Consultant Credibility

www.twdb.texas.govwww.wetland.co.nz

www.ysi.com

INVESTING IN THE FUTURE

www.algaeindustrymagazine.com

Creation of the Process Water MarketsDirector: Camilo DenBrok

PWM Application EngineerMichael Cohrs

Grant ProgramsMarine and Freshwater ResearchAlgae Technology ResearchLife Sciences and Materials Research

"We at Fluid Imaging Technologies believe there are multiple opportunities to enhance process control in the water and wastewater market resulting in time and cost savingsby using our game-changing dynamic imaging particle analysis technology, FlowCAM."

Water Treatment Applications

Waste Water Applications

ON THE DRAWING BOARDIN COLLABORATION WITH INDUSTRY LEADERS AND ACADEMIC COMMUNITIES

Floc Analysis

Microbial Floc Analysis

Zooplankton

Invasive Species

Filter Fouling Algae

Grit Removal

CyanoCAM

Filter Medium Analysis

Taste and Odor Issues

Macroscopic Particulate Analysis

Stormwater Particulate Analysis

Wastewater Treatment Control

Water Treatment Management and Wastewater Treatment Applications

www.kotaku.com.au

QUESTIONS AND COMMENTS

Fluid Imaging Technologies200 Enterprise DriveScarborough Maine, 04074

Camilo DenBrokFluid Imaging Technologies

Director of Sales, Process Water Markets camilo.denbrok@fluidimaging.com

404-414-1636

Michael CohrsFluid Imaging Technologies

Applications Engineer, Process Water MarketsMichael.cohrs@fluidimaging.com

(973) 650-6152