Career Seminar Series IVF Embryologist Dr Gayle M. Jones Senior Research Fellow Monash Immunology...

Career Seminar SeriesCareer Seminar Series

IVF EmbryologistIVF Embryologist

Dr Gayle M. JonesSenior Research Fellow

Monash Immunology & Stem Cell Laboratories

Tertiary TrainingTertiary Training

• B.Sc.(HONS) University of Melbourne• Ph.D. Department of Pathology, University

of Melbourne• Certificate Recombinant DNA Techniques

– Micromon, Monash University

Career PathCareer Path• NH&MRC Senior Technical Officer – Royal Women’s

Hospital• NH&MRC Research Officer – Royal Women’s Hospital• IVF Embryologist – Royal Women’s Hospital• Visiting Research Fellow – UC Irvine, California• Director of Embryology – Melbourne IVF• Research Fellow – Monash Institute of Reproduction &

Development• Senior Research Fellow – Monash Institute of

Reproduction & Development• Senior Research Fellow – Monash Immunology & Stem

Cell Laboratories

Career Opportunities for Scientists Career Opportunities for Scientists in Assisted Reproductionin Assisted Reproduction

• Clinical Embryologist/Preimplantation Geneticist• Laboratory Scientist – Pathology

• Andrology• Endocrinology

• Research Scientist• Human• Animal species

Day 0 – Oocyte RetrievalDay 0 – Oocyte Retrieval

Day 0 - InseminationDay 0 - Insemination

Intra cytoplasmic sperm injection - ICSIIntra cytoplasmic sperm injection - ICSI

Day 1 – Fertilization checkDay 1 – Fertilization check

Embryo Culture – Day2-Day5/6Embryo Culture – Day2-Day5/6

Embryo TransferEmbryo Transfer

EMBRYO BIOPSYEMBRYO BIOPSY

1st PB

2nd PB

ICSIICSI

1-2 / 8 cellsTrophectoderm

20 cells

Day 0Day 0

Day 1Day 1

Day 3Day 3

Day 5Day 5

Hole in the zona pellucida by non-

contact laser

Insertion of glass micropipette

Removal of a single blastomere for genetic

analysis

Embryo Biopsy – Day 3 EmbryoEmbryo Biopsy – Day 3 Embryo

XX XY XXX

Fluorescence In Situ Hybridization

XX XY XXX

XO Monosomy 21 Trisomy 21

Results of Clinical PCR for Cystic Results of Clinical PCR for Cystic Fibrosis – Day 3 EmbryosFibrosis – Day 3 Embryos

Father (carrier)

Embryo (carrier)

Embryo (affected)

Embryo (unaffected)

Mother (carrier)Delta F508 Normal