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Advanced Light MicroscopyHarvard Center for Biological Imaging
Room 2052 Biological Laboratories 16 Divinity Ave Cambridgewwwhcbifasharvardedu
About the HCBI LightsheetLightsheet Z1Dual camera incubation for live samples optics for cleared tissue
XY
AMulti-photon
MIP
Insight DS+ on upright LSM 880Tunable from 680 to1300 nm enables 2-photon 3-photon SHG and THG imaging
ConfocalLSM 880 upright amp inverted Airy scan spectral unmixing reection confocal FLIM live cell incubation and focus maintenance
Cleared tissue imaging
Laser capture dissection
PALM microbeam 3 laser dissectionBrighteld or uoresence dissectioncatapultcapture to Eppindorf tubes
Axio ScanZ1 whole slide scanner 100 slide capacity bright eld5 color uorescence polarization
High-content screening
Spectral unmixing100
Rela
tive
inte
nsity
()
450 550 850Wavelength (nm)
50
650
Ale
xa 5
14
Ale
xa 5
55
Ale
xa 5
68A
lexa
594
Ale
xa 6
33
Ale
xa 6
47
Ale
xa 6
60
Porphyromonas endodontalisPrevotella OrisParvimonas micraActinomyces sp 169
Fusobacterium nucleatumStreptococcus sanguinisVeilonella parvula
Cell Discoverer 7 automated imagingbrighteld 4 uorescent channelsrobotic loading of 20+ platesfull incubation and focus maintenance
xed cell screening
live cell imaging
wel
l 1
wel
l 6
1 mm
0 hr 95 hr 195 hr
wel
l 1
wel
l 6
1 mm
0 hr 95 hr 195 hr
13 Imaging Platforms 5 Workstations Cloud Data Storage lsquoEvergreenrsquo facility microscopes replaced every 24-36 months Open to all researchers regardless of aliation Free training Volume discounts for heavy users and live-cell imaging On-site support from imaging experts
Super-resolution
SIM
WF
WF
PALM
SIM
PALM
ELYRA PS1 Super-resolutionTIRF Structured IlluminationSingle Molecule Localization (PALM STORM)
50 nm
100 nm
AI-based segmentation
multi-well plate live imaging
X-CLARITY Hydrogel embeddingElectrophoretic Tissue Clearing
Smart Clear ProElectrophoretic Tissue ClearingElectrophoretic Antibody staining (Jan 2019)
GPU-accelerated deconvolution
Contact UsErin Diel PhD Imaging Specialist617-495-2257 | edielfasharvardeduChristian Hellriegel PhD Embedded Zeiss Specialist617-496-6250 | christianhellriegelzeisscomDouglas Richardson PhD Director of Imaging617-496-5422 | drichardsonfasharvardedu
Macroscopy
Education
Axio Zoom V16 uorescence + optical sectioningbrighteld darkeld polarization
MCB 68 - Cell Biology through the microscope MCB 352 - MicroscopyBi Annual Tissue Clearing Workshop (fall 2019)Lunch and Learn Lecture Series (posted to HCBI YouTube channel)
Harvard Center for Biological Imaging99 subscribers
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