Advanced Light Microscopy Harvard Center for Biological Imaging Room 2052 Biological Laboratories, 16 Divinity Ave, Cambridge www.hcbi.fas.harvard.edu About the HCBI Lightsheet Lightsheet Z.1 Dual camera, incubation for live samples, optics for cleared tissue XY A Multi-photon MIP Insight DS+ on upright LSM 880 Tunable from 680 to1300 nm, enables 2-photon, 3-photon, SHG and THG imaging Confocal LSM 880 upright & inverted Airy scan, spectral unmixing, reflection confocal, FLIM, live cell incubation and focus maintenance Cleared tissue imaging Laser capture dissection PALM microbeam 3 laser dissection Brightfield or fluoresence dissection catapult/capture to Eppindorf tubes Axio Scan.Z1 whole slide scanner 100 slide capacity, bright field 5 color fluorescence, polarization High-content screening Spectral unmixing 100 Relative intensity (%) 450 550 850 Wavelength (nm) 50 650 Alexa 514 Alexa 555 Alexa 568 Alexa 594 Alexa 633 Alexa 647 Alexa 660 Porphyromonas endodontalis Prevotella Oris Parvimonas micra Actinomyces sp. 169 Fusobacterium nucleatum Streptococcus sanguinis Veilonella parvula Cell Discoverer 7 automated imaging brightfield, 4 fluorescent channels, robotic loading of 20+ plates, full incubation and focus maintenance fixed cell screening live cell imaging well #1 well #6 1 mm 0 hr 9.5 hr 19.5 hr well #1 well #6 1 mm 0 hr 9.5 hr 19.5 hr · 13 Imaging Platforms, 5 Workstations, Cloud Data Storage · ‘Evergreen’ facility: microscopes replaced every 24-36 months · Open to all researchers regardless of affiliation · Free training · Volume discounts for heavy users and live-cell imaging · On-site support from imaging experts Super-resolution SIM WF WF PALM SIM PALM ELYRA PS1 Super-resolution TIRF, Structured Illumination, Single Molecule Localization (PALM, STORM) 50 nm 100 nm AI-based segmentation multi-well plate live imaging X-CLARITY Hydrogel embedding Electrophoretic Tissue Clearing Smart Clear Pro Electrophoretic Tissue Clearing Electrophoretic Antibody staining (Jan 2019) GPU-accelerated deconvolution Contact Us Erin Diel, PhD Imaging Specialist 617-495-2257 | [email protected] Christian Hellriegel, PhD Embedded Zeiss Specialist 617-496-6250 | [email protected] Douglas Richardson, PhD Director of Imaging 617-496-5422 | [email protected] Macroscopy Education Axio Zoom V16 fluorescence + optical sectioning brightfield, darkfield, polarization MCB 68 - Cell Biology through the microscope MCB 352 - Microscopy Bi Annual Tissue Clearing Workshop (fall 2019) Lunch and Learn Lecture Series (posted to HCBI YouTube channel) Harvard Center for Biological Imaging 99 subscribers

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Advanced Light MicroscopyHarvard Center for Biological Imaging

Room 2052 Biological Laboratories 16 Divinity Ave Cambridgewwwhcbifasharvardedu

About the HCBI LightsheetLightsheet Z1Dual camera incubation for live samples optics for cleared tissue

AMulti-photon

MIP

Insight DS+ on upright LSM 880Tunable from 680 to1300 nm enables 2-photon 3-photon SHG and THG imaging

ConfocalLSM 880 upright amp inverted Airy scan spectral unmixing reection confocal FLIM live cell incubation and focus maintenance

Cleared tissue imaging

Laser capture dissection

PALM microbeam 3 laser dissectionBrighteld or uoresence dissectioncatapultcapture to Eppindorf tubes

Axio ScanZ1 whole slide scanner 100 slide capacity bright eld5 color uorescence polarization

High-content screening

Spectral unmixing100

Rela

tive

inte

nsity

()

450 550 850Wavelength (nm)

650

Ale

xa 5

Ale

xa 5

Ale

xa 5

68A

lexa

594

Ale

xa 6

Ale

xa 6

Ale

xa 6

Porphyromonas endodontalisPrevotella OrisParvimonas micraActinomyces sp 169

Fusobacterium nucleatumStreptococcus sanguinisVeilonella parvula

Cell Discoverer 7 automated imagingbrighteld 4 uorescent channelsrobotic loading of 20+ platesfull incubation and focus maintenance

xed cell screening

live cell imaging

wel

l 1

wel

l 6

1 mm

0 hr 95 hr 195 hr

wel

l 1

wel

l 6

1 mm

0 hr 95 hr 195 hr

13 Imaging Platforms 5 Workstations Cloud Data Storage lsquoEvergreenrsquo facility microscopes replaced every 24-36 months Open to all researchers regardless of aliation Free training Volume discounts for heavy users and live-cell imaging On-site support from imaging experts

Super-resolution

SIM

PALM

SIM

PALM

ELYRA PS1 Super-resolutionTIRF Structured IlluminationSingle Molecule Localization (PALM STORM)

50 nm

100 nm

AI-based segmentation

multi-well plate live imaging

X-CLARITY Hydrogel embeddingElectrophoretic Tissue Clearing

Smart Clear ProElectrophoretic Tissue ClearingElectrophoretic Antibody staining (Jan 2019)

GPU-accelerated deconvolution

Contact UsErin Diel PhD Imaging Specialist617-495-2257 | edielfasharvardeduChristian Hellriegel PhD Embedded Zeiss Specialist617-496-6250 | christianhellriegelzeisscomDouglas Richardson PhD Director of Imaging617-496-5422 | drichardsonfasharvardedu

Macroscopy

Education

Axio Zoom V16 uorescence + optical sectioningbrighteld darkeld polarization

MCB 68 - Cell Biology through the microscope MCB 352 - MicroscopyBi Annual Tissue Clearing Workshop (fall 2019)Lunch and Learn Lecture Series (posted to HCBI YouTube channel)

Harvard Center for Biological Imaging99 subscribers