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The Fluorescence Dive Experience
• Beauty
• Transformation
The Fluorescence Dive Experience
• Transformation
The Fluorescence Dive Experience
The Fluorescence Dive Experience
• Transformation
The Fluorescence Dive Experience
• Transformation
The Fluorescence Dive Experience
• Transformation
The Fluorescence Dive Experience
• Transformation
• Focus ofattention
Photograph courtesy of SanjayJoshi
The Fluorescence Dive Experience
The Fluorescence Dive Experience
• Focus ofattention
The Fluorescence Dive Experience
• Focus ofattention
• Discovery
Copyright 2012 Gary Morris
The Fluorescence Dive Experience
Fluorescence Diving
• What fluorescence is.
• What equipment you need to see or photograph
fluorescence.
• What you are going to see on your fluorescence dive.
• What good is fluorescence?
– For the animal
– For the scientist
• Fluorescence where you are
What is Fluorescence?
Luminescence
Emission of light without heat
Bioluminescing dinoflagellate
E. Widder, HBOI
Fireflies!
LUMINESCENCE
BIOLUMINESCENCE
Makes its own light
What is Fluorescence?
Makes its own light Glows when you shine light on it
LUMINESCENCE
BIOLUMINESCENCE Fluorescence Phosphorescence
What is Fluorescence?
What is Fluorescence?
Glow stopswhen you turnoff the light
Glow continueswhen you turn offthe light
Makes its own light Glows when you shine light on it
LUMINESCENCE
BIOLUMINESCENCE Fluorescence Phosphorescence
• Fluorescence is the absorption ofelectromagnetic radiation (light) at onewavelength, and its re- emission at another, longer wavelength
FluorescenceUV
longer wavelength lower energy
shorter wavelength higher energy
Fluorescence
How to Image Fluorescence – Eye orCamera
Barrier filter
Exciter filter
Imaging
device
Excitation source
Seeing Fluorescence
Photographing Fluorescence
Fluorescence in the Daytime
Making a Fluorescence Dive
• Night
• Use your white light to navigate and orient yourself
• Switch over to blue light, make sure you have the
yellow filter in front of your eyes
• Use your white light only as needed to:– Re-orient yourself
– Signal your buddy (they won' t see the blue!)
– Find out what it is that is fluorescing
• Etiquette – don' t point your white light where your buddy or other fluorescence divers are looking
• Discover a new world
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
What You Will See on Your Dives
e N i htSea
What You Will See on Your Dives
Photograph courtesy of Jason Heller
What You Will See on Your Dives
World' s First Fluorescent Shark!!
What is Fluorescence Good For?
What is Fluorescence Good For?
What is Fluorescence Good For?
Fluorescence Finds Small Targets
Fluorescence Finds Small Targets
Fluorescence Finds Small Targets
Application – Coral Recruitment Research
Application – Coral Recruitment Research
e N i htSea
• This presentation supplied by NightSea LLC exclusively for
Fluorescence AWARE Diver copyright 2011
• NightSea develops equipment for recreational and scientific divers to see and photograph fluorescence underwater
• For more information about fluorescence, fluorescence
viewing or fluorescence photography equipment:
NightSea & Fluorescence AWARE
Websites
www.nightsea.com
www.fluorescenceaware.com
