SNP GENOTYPING

Whitney Barfield and Daniel Salahuddin

Genotyping

Genotyping provides a measurement of the genetic variation between members of a species

SNP Example

PCR

Genotyping

The light emitted from the dye in the excited state is received by a computer.

Real-time PCR Machine PCR

The Read-Out

SNPSWhitney Barfield and Daniel Salahuddin

SNP SPP1

SPP1 binds and alters activity of a metalloproteinase

Metalloproteinase Family of genes

involved in adipose tissue remodeling

Linked to measures of obesity

Secreted Phosphoprotein 1 Obesity

SNP ATP2A1

ATPase, Ca++ transporting, cardiac muscle, fast twitch 1(ATP2A1) Involved in muscular

excitation and contraction

Brody disease (BD) inherited disorder of

skeletal muscle function Exercise induced symptoms

ATPase Clinical Symptoms

SNP MTCH2

Mtch2-resident complex participates in the mitochondrial apoptotic program.

Programmed cell death, or apoptosis is critical for both the

development and maintenance of tissues

Mitochondrial Carrier Homolog 2

(C. elegans)Caenorhabditis elegans