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2012 Presentation

Engeenuity presentation 2012 feb

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2012 Presentation

The Engeenuity Technology inventors of the Advanced Physics Systems Engineering (APSE™)Solution to Predictably Control Pathogens in Food Products & Water

The Engeenuity process configures (a) naturally-occurring forms of light, (b) earth’s naturally-occurring magnetic field, and (c) temperature and other naturally-occurring elements to inhibit pathogen growth in food products and water (and to enhance the food and water in other ways). The all-natural, organic process is:

Non-chemicalNon ToxicNon-invasive (no physical contact with the food / water being treated is

required in some applications)Self-sustaining (no artificial power source required to produce or use)Safe for humans, (as per FDA standards)Portable Inexpensive

Current ActivityCooling Tower Treatment ,Water with an cooling tower operations

lab & management company Cooling towers in live operation have established the

Engeenuity process to be effective at maintaining microbial populations below acceptable levels on a consistent basis for over 46 days.

Cooling Tower Testing validated by a very large, international water provider and operations management company

Current ActivityExtending Shelf Life in Bread in the largest commercial

bakers in North America

Lab / bench top tests have confirmed that the Engeenuity Process inhibits mold growth in bread products to add up to 11 days of shelf life to the product.

(Full's Scale Production Lines installed 2009)

Current ActivityWastewater testing with two large wastewater services

companies.

Mid-stage testing has established that the Engeenuity process accelerates the sedimentation of wastewater in a plant’s clarifier up to 30% by, in part, interfering with the filamentous bacteria (without harming the desired higher forms of life in the wastewater).

Shows impact on e coli, total coliforms, pH, DO, DO saturation, TDS, ORP

What is the Engeenuity process?

Engeenuity uses technology APSE™ combining Optical absorption / photoconductivity /photonics/pulsed light /interfered magnetic fields /temperature for food preservation technology in 2 ways.

First, It’s not just about magnetic fields. Engeenuity combines

naturally occurring phenomena with Optical absorption and Photoconductivity

It’s about…..

the earth’s natural magnetic field configured using natural

interference patterns

It’s about….. Capturing and storing Solar Energy / photons & other solar

elements (using NASA-generated solar activity data)

(Tested at the International Institute of Biophysics/ Neuss, Germany, and shown to store high levels of light)

It’s about…..

Nano-Light Pulsations frequencies that occur as a result of the different and possible formulas configured each time according to current activities, including solar activity, for specific end purposes forming photo-induced radicals over a certain period of time.

Second, The Enhanced APSE™ Field is Now Portable. The Engeenuity Processing Steps are:

The enhanced APSE field is prepared in the “source unit” by the use of optical engineering & instrumentation and by the use of physics, geophysics, photonics & mathematical models data.

The “source unit” prepares the enhanced magnetic field by attracting and transforming (through natural interference patterns) the earth’s naturally occurring magnetic field, photons and combining it with other naturally occurring elements, such as Solar energy and Temperature.

The operator of the “source unit” is able to reconfigure the internal components of the “source unit” in order to achieve each desired application

The “source unit” requires no outside power to operate

The Engeenuity Process is Now Portable

The “source unit” embeds the enhanced field to a gel / paste-like suspension that contains silver ions and combining Photo dynamics using photosensitizing agents elements, (and other materials to transmit light). This suspension is able to store the enhanced magnetic field, as well as facilitate its recharge. The suspension material is small and portable, and may be deployed separately and apart from the “source unit”.

How does the Engeenuity Process have an impact on

pathogenic cells and molds?

Established science accepts that all living organisms emit an

electromagnetic / biophotonic wave. Science has even

measured the frequencies of particular types of organisms.

This is the core of how the Engeenuity technology works.

The enhanced magnetic fields disrupt targeted pathogens,

but without altering healthy parts of the subject target.

How does the Engeenuity Process have an impact onpathogenic cells and molds?

FDA Report:

U.S. Food and Drug AdministrationCenter for Safety and Applied NutritionJune 2, 2000 _________________________________________Kinetics of Microbial Inactivation for Alternative Food Processing

Technologies

“…SMF and OMF may have some potential to inactivate microorganisms in food.”

“…A review of the literature shows that inconsistent results have been obtained on the effect of OMF on microbial growth.”The report does not question the safety of the use

of magnetic fields.

How does the Engeenuity Process have an impact on

pathogenic cells?

The FDA Report suggests 3 theories:

The “protein ion” theory. Many proteins vital to cell metabolism contain ions. At certain frequencies, a magnetic field may loosen the bond between ions and proteins.

The “calcium ion / protein” theory. This is the “protein ion” theory made specific as to the calcium ions in certain calcium-binding proteins.

The “DNA” theory. The energy from the magnetic field causes the covalent bonds in the DNA molecule to breakdown, which, in turn, inhibits growth of pathogenic microbes.

How does the Engeenuity Process have an impact on

pathogenic cells?Another theory - - Fritz Albert Popp / Intl. Institute of Biophysics / Neuss-

Germany Coherence Theory of Biophotonics

Biophotonic emissions (electromagnetic waves) from organic systems are not meaningless by-products, but rather are part of a coherent communication system

Popp deals with extremely high frequency electromagnetic waves. Engeenuity works at the very opposite end of the spectrum. But the concept of a non-chemical / non-electric communication mechanism may apply to both. ~University of Chicago study published in Bioelectrochemistry in November 2007.

Whether or not the coherent communication theory is at work

with Engeenuity’s technology, there is a residual effect, which is best introduced by example.

Examples of EFFECTIVENESS - - Silliker Water Study

Engeenuity APSE technology controlled the growth of inoculated Escherichia Coli cultures in water

Silliker inoculated water with the following E Coli cultures:

The inoculated water was passed - -3 times - - through a coil that was lined with the portable Engeenuity card.

Cultures Culture Name

Escherichia Coli 434 (ATCC26)

Escherichia Coli 1110 (ATCC8739)

Escherichia Coli 1490 (ATCC11775)

Examples of EFFECTIVENESS - - Silliker Water Study

The untreated, inoculated control water reached the spoilage level of 10 million cfu/ml (or gram) in 14 hours (at room temperature).

The treated, inoculated water that passed through the Engeenuity coil 3 times did not reach the spoilage level of 10 million cfu/ml (or gram) until 96 hours (at room temperature).

Samples of the treated water after 1 pass through the coil and after 2 passes were also drawn and tested. These performed very well compared to the control, but not as well as the water that passed through the coil 3 times.

Effectiveness and control of the process.

Examples of EFFECTIVENESS - - Engeenuity APSE technology has consistently controlled microbial growth in milk - - as verified by independent laboratory studies

HML, Inc. Laboratory study conducted September 2007 through January 2008. Milk treated by the Engeenuity enhanced APSE field was microbiologically safe to drink 93 days after the stated expiration date on the carton. Control sample “spoiled” 23 days after the stated expiration (possibly sooner).

Rules of thumb for milk microbial safety and curdling (from Cornell University, Food Science Department):

Milk “spoils” when the Aerobic Plate Count exceeds 10,000,000 cfu/ml

New, fresh milk has a pH in the range of 6.4 to 6.8

Milk curdles at a pH of 4.5

HML, Inc. Laboratory December 17, 2007 Test Sample

Test Method Result Date Sample Drawn

Aerobic Plate Count

FDA 840,000 cfu/ml

12/17/2007 (69 days after expiration)

Psychrotrophic APC

FDA 160,000 cfu/ml

12/17/2007 (69 days after expiration)

Lactic Acid Bacteria

FDA 200,000 cfu/ml

12/17/2007 (69 days after expiration)

Yeast & Mold Count

FDA < 1 cfu/ml 12/17/2007 (69 days after expiration)

pH FDA 5.78 12/17/2007 (69 days after expiration)

HML, Inc. Laboratory January 2008 Test Sample

(after carton was opened on 12 / 17 / 2007)

Test Method Result Date Sample Drawn

Aerobic Plate Count

FDA 450,000 cfu/ml

1/10/2008 (93 days after expiration)

Psychrotrophic APC

FDA 1,520,000 cfu/ml

1/10/2008 (93 days after expiration)

Lactic Acid Bacteria

FDA 420,000 cfu/ml

1/10/2008 (93 days after expiration)

Yeast & Mold Count

FDA < 1 cfu/ml 1/10/2008 (93 days after expiration)

pH FDA 5.72 1/10/2008 (93 days after expiration)

SENSITIVE & FLEXIBLE - - Silliker, Inc. Milk Study

Silliker tested milk by attaching an Engeenuity portable card to a carton of milk, and compared this to an untreated control carton.

The test ran for 34 days, and the treated milk demonstrated a 1 to 2 log reduction in APC relative to the control.

pH of the treated sample was 5.6 (no curdling). The untreated control sample curdled at 4.2.

Silliker concluded: “Overall, higher pH value and lower aerobic

plate count suggested that the laminated microchip device had a positive effect to extend the shelf life of milk.”

SENSITIVE & FLEXIBLE - - Silliker, Inc. Milk Study

This Silliker study did, however, point out a possible weakness with the Engeenuity portable card. The thermoduric psychrotrophs were substantially above limit in both the treated and the untreated control.

Armed with this information, the “source unit” was reconfigured for application to milk, with the result that in a study with the Agricultural Research Service of the USDA, the psychrotrophs were controlled (and also controlled in the HML, Inc. study).

U.S. Department of AgricultureAgricultural Research Service

  MATERIAL TRANSFER AGREEMENT

The “source unit” is not a static, blunt instrument. It is adjustable to the situation.

SENSITIVE & FLEXIBLE - - Silliker, Inc. Ground Beef Study

Engeenuity focused on the fast food industry for this study.Silliker tested 4 different thicknesses of hamburgers.This study was performed by leaving the ground beef at

room temperature for 24 hours.The 1.0 cm hamburger in the study represented the typical

fast food burger thickness, showed 96% lower APC than did the untreated control. By request this is the burger that Engeenuity targeted in this study.Thickness of

BurgerZero Hour Count (cfu/g)

Control Count at 24 Hours (cfu/g)

Treated Count at 24 Hours (cfu/g)

% Difference

0.3 cm 410,000 210,000,000

13,000,000 93.8%

1.0 cm 410,000 210,000,000

7,200,000

96.6%

1.5 cm 410,000 210,000,000

45,000,000 78.6%

2.5 cm 410,000 210,000,000

210,000,000

0%

SENSITIVE & FLEXIBLE - - Silliker, Inc. Milk & Ground Beef Studies

The point and the significance of the sensitivity and flexibility of the Engeenuity technology is two-fold.

First, we can target the specific needs for a company. There will be multiple applications and methods a company can use and benefit from the technology.

Second, while we have excellent portable devices for the initial testing of this application, fine tuning may be beneficial after initial results are obtained.

RESIDUAL / CONTINUING EFFECT - - Silliker, Inc. Milk Study (coil pass through application)

In this study, milk was treated by separating a small portion of a one-gallon carton of milk. This small portion was poured through a coil that was lined with the Engeenuity portable card. This milk sample was poured through the coil 3 times.

The milk treated through the coil was then returned to its original untreated carton at a ratio of 4.5 (treated milk) to 95.5 (untreated).

RESIDUAL / CONTINUING EFFECT - - Silliker, Inc. Milk Study (coil pass through application)

The treated sample was compared to the untreated control gallon.

The APC of the control was 14 ½ times that of the treated sample.pH of the treated sample was 6.4, compared to a 5.0 pH for the

control.The peroxide value (to indicate the extent of oxidation) of the control

was 2 ¾ times that of the treated sample (the treated sample’s PV increased only very slightly).

Residual effect / biophotonic Coherence Theory / communication.

Prof. Dr. Georg Kurz GmbH - - Beef & Pork Mixture Tests

Engeenuity conducted a series of tests at the laboratory of Prof. Dr. Georg Kurz GmbH (a well-regarded food science lab in Cologne, Germany) as to a mixture of beef and pork.

Treated samples were exposed to the Engeenuity portable device in a refrigerator for a 24 to 48 hour period. The meat samples were then tested for APC.

Very favorable results.

Examples of EFFECTIVENESS - - Commercial bakery; Water; Wastewater; Cooling Towers

Please review “Current Activity” slides.

Intellectual Property / Provisional Patent Filing

U.S. Provisional Patent Application filed.

Non-Disclosure Agreements.

Thanks You For Your Time