Electrodeposition of flower-like nickel oxide onCVD-grown graphene to develop an

electrochemical non-enzymatic biosensor

Arunkumar Rengaraj,‡a Yuvaraj Haldorai,‡b Cheol Hwan Kwak,a Seungbae Ahn,cKi-Joon Jeon,c Seok Hoon Park,d Young-Kyu Han*b and Yun Suk Huh

aDepartment of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Republic of Korea

bDepartment of Energy and Materials Engineering, Dongguk University-Seoul, Republic of Korea

cDepartment of Environmental Engineering, Inha University, Republic of Korea

J. Mater. Chem. B

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Introduction

Cholesterol

Lipid molecule

Required to maintain both membrane

structural integrity and fluidity

plantcentric.wordpress.com

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

www.cellbiol.net

Introduction

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Introduction

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

• The only problem with the graphene is that high quality graphene is a great conductor that does not have a band gap (It cant be switched off).

• To use Graphene in the creation of future nana electronic devices ,a band gap will need to engineered.

Introduction

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

• A mediator is a low molecular weight redox couple which can transfer electrons from the active site of the enzyme to the surface of the electrode, thereby

establishing electrical contact between the two.

• The purpose of using metallised electrodes is to créate conditions in which the oxidation of enzymatically generated H2O2 can be achieved at a lower applied

potential, by creating a highly catalytic surface.

• In addition to reducing the effect of interferents, due to the lower applied potential, the signal-to-noise ratio is increased due to an increased electrochemically

active area.

• Nickel 's high electrical conductivity lends itself to many electronics applications.

• Nickel oxide/active carbon composites as the electrode materials for super-capacitors 

NiO as a mediator

Introduction

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Mechanism of Cholesterol sensor

Scheme

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Characterization

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Characterization

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Characterization

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

ElectrochemicalCharacterization

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Sensing of Cholesterol

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Sensing of Cholesterol

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Stability of the sample

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Comparison

Inha universityDepart. Of Biological EngineeringNano-Bio Analysis Lab, Yun's Group

Conclusion