Jeremy J. Mao

Columbia University

3D Printed Materials in Vivo: Healing

by Endogenous Stem Cells

April 11, 2016

New York, NY

§ One in five adults diagnosed of arthritis

§ Half of those 65 or older

§ By 2030, ~67 million Americans (>18)

§ Could 3D printing be a solution for arthritis

§ ~22 people die each day waiting for organ transplants

§ What does 3D printing have to do with organ transplantation

a b

cgf

Bone

d e

400 µm 200 µm

Cartilage

80 wt% poly-ε-caprolactone (PCL) (Mw ~65,000)

20 wt% of hydroxyapatite (HA)Lee et al. 2010 Lancet Aug 7;376(9739):440-8. PMID: 20692530

f g

h i

Lee et al. 2010 Lancet Aug 7;376(9739):440-8. PMID: 20692530

Lee et al. 2010 Lancet Aug 7;376(9739):440-8. PMID: 20692530

Lee et al. 2010 Lancet Aug 7;376(9739):440-8. PMID: 20692530

Nature Reviews Rheumatology 6, 677-678 (2011)

Daniel A. Grande, Nicholas A. SgaglioneRegenerative medicine: Self-directed articular resurfacing: a new paradigm?

Recruitment

Cell homing

Differentiation

>1M meniscus repairs/yr

Meniscectomy (+/- allograft)

1o factor for osteoarthritis

(100 μm) (50 μm)

C DA B

E F

Day 1 Day 8

Dextran (40 kDa)

Dextran (10 kDa)

G

A B

15 mm

C

D E

Scott Rodeo, HSS

Lisa Fortier, Cornell

Nati

ve

+ C

TG

F/T

GF

β3-µ

S

Outer Middle Inner

BA

ED

C

F

(100μm)

Scott Rodeo, HSS

Lisa Fortier, Cornell

A B C

Root canal

therapy

BioPulp

To Avoid Root Canals, Teeth That Replace

Themselves

Stem-Cell Research Makes Progress in Quest to

Avoid the Dreaded Drill

§ 18 million root canal

patients/yr

§ $22B annual

expenditure

2014

https://www.google.com/search?q=organ+shortage&espv=2&biw=1209&bih=704&source=lnms&tbm=isch&sa=X&ved=0ahUKEwi2wfze2oTMAhVLGT4KHcy5CPMQ_AUIBygC#imgrc=8cEvQPUTai1AvM%3A

3D printing

Organ shortage

3D printing…holds considerable

promise in generating implantable

organ scaffolds that may reduce or

eliminate organ shortage.