Role of synthesized Zinc Oxide nanoparticles on the

germination and growth characteristics of Mungbean seeds (Vigna radiata)

Presented by: Camellia Roy 3rd year B.Sc Biotechnology (H) Gurunanak Institute of Pharmaceutical

Science & Technology

Overview Nanotechnology

Synthesis of Zinc oxide : i) Chemical wet method ii) Green synthesis

method

The beneficial effect of Zinc oxide nanoparticles prepared for the treatment of mungbean (Vigna radiata) seeds.

Results and Discussions

Conclusion

References

One nanometer is a billionth of a meter, or 10-9 of a meter.

Examples:

Figure 1: Illustrative examples of nanotechnology

Meet Nano!

Physicist Richard Feynman, the father of nanotechnology “There’s Plenty of Room at the Bottom ”Professor Norio Taniguchi coined the term nanotechnology.

Synthesis of Zinc oxide nanoparticles Chemical wet method ZnCl2 + 2NaOH Zn (OH)2 + 2NaCl Zn (OH)2 ZnO + H2O

   

Figure 2: Nano Zinc oxide particles synthesized by chemical wet method

Continue.

Green synthesis method BA

C

D

Figure 3: Synthesis of ZnO Nanoparticles using leaf extract of Camellia sinensis (green tea)

The beneficial effect of Zinc oxide nanoparticles prepared for the treatment

of mungbean (Vigna radiata) seeds

BA C

Figure 4: A) Mungbean seeds (B) Seedling exposure (C) Mungbean sprouts

Results and discussions

Figure 5: A) Different concentrations of ZnO NPs on Mungbean seed germination (B) Effect of

ZnO NPs on seed germination

A B

Biomass weight(gm)

Shoot Length (cm)

Root Length (cm)

Shoot Weight (gm)

Root Weight (gm)

Control 0.425 20.4 7.4 0.291 0.02050 mg 0.536 22.2 8.9 0.322 0.022.8100 mg 0.532 23.5 9 0.332 0.023150 mg 0.526 20.4 9.4 0.318 0.020.2

Continue..

Figure 6: Mungbean seed germination and measurement of growth parameters within 20

days

Conclusion

Environment eco-friendly synthesized zinc oxide nanoparticles effect on the mung bean seed germination. It penetrates easily inside the plant cell wall and causes effects on biomass and growth parameters of mung bean plant. Deposition of small size nanoparticles inside the cell wall and vacuoles causes disturbance in metabolic activity of plant. Mungbean (Vigna radiata) are economically important crop plant, nanoparticles can easily find their way in human body through food chain. More investigations are needed to determine the positive and negative impact of nanoparticles on crop plant and its consequences in other living organisms.

ReferencesA Khorsand Zak, R Razali, WH Abd Majid, Majid Darroudi, (2011). Synthesis and Characterization of a Narrow size Distribution of Zinc Oxide Nanoparticles, International Journal of Nano medicine, Vol, 6, pp. 1399–1403.

F.Hong, L. Wang, X. Meng, Z. wei, G. (2005) Zhao, Effect of Nano-TiO2 on photochemical reaction of chloroplasts of spinch, Biological Trace Elements Resaerch, vol.8

Gianquinto G, Azmi AR, Tola LD, (2000). Interaction effects of phosphorus and zinc on photosynthesis, growth and yield of dwarf bean grown in two environments. Plant Soil, vol.28.

K. Suresh babu and V. Narayanan, (2013). Hydrothermal Synthesis of Hydrated Zinc Oxide Nanoparticles and its Characterization Chem Sci Trans., Vol, 1 pp. S33-S36

.Navarro, E., Baun, A., Behra, R., Hartmma N.B., Filser, (2008) Environmental behavior and eco-toxicity of engineered nanoparticles to algae, plants and fungi. Ecotoxicology, vol. 17.

Liu XM, Zhang FD, Zhang SQ, (2010). Effects of nano-ferric oxide on the growth and nutrients absorption of peanut. Plant Nutr Fert Sci, vol. 11.

S.R. Senthilkumar, T. Sivakumar, (2014).Green tea(Camellia sinensis) mediated synthesis of Zinc oxide (ZnO) nanoparticles and studies on their antimicrobial activities, Vol 6,Issue 6,ISSN-0975-1419

K. Prasad1, Anal K. Jha.(2009), ZnO Nanoparticles,Synthesis and Adsorption Study. Natural Science,Vol, 1,pp. 129-135 

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