FIELD APPLICATION OF METAL-MICROBE INTERACTIONPresented By AHMED MADNIRegistration No. SP14-BTY-011

05/03/2023

2

CONTENTS Introduction Bioleaching Types of Bioleaching Species used in Bioleaching Mineralization Famous company currently working Research Future prospects

05/03/2023

3

INTRODUCTION

Bioleaching and Bioremediation of polluted sites

Mineralization of polluting organic

compounds

05/03/2023

4

05/03/2023

5

BIOLEACHING

05/03/2023

6

CONTINUE… Bioleaching works by using specific bacteria

that can essentially “eat” the metal content out of ore.

It is carried when there are lower concentrations of metal in ore and they need an efficient, environmentally responsible method.

05/03/2023

7

TYPES OF BIOLEACHINGBased on mechanism

Direct Bioleaching (Enzymes involved)

Indirect Bioleaching (Chelating substances)

05/03/2023

8

05/03/2023

9

BACTERIA USED IN BIOLEACHINGSpecies used is Thiobacillus Ferrooxidants Acidophilic chemolithoautotrophs that utilize

inorganic compound as energy source. Organic acid and chelating molecules are

produced

05/03/2023

10

oxidizing of pyrite (FeS2)

05/03/2023

11

Urinate ore to uranium

Oxidizes insoluble to

soluble

05/03/2023

12

CONTINUE… Microbe reducible metal Produces Ferric iron and that forms complex structure and make it soluble form at neutral pH Can be used as terminal electron acceptor in in

situ anaerobic bioremediation of polluted site

Extraction of metals such as Co, Mo, Ni, Pb and Zn from sulfide ores by bioleaching is technically feasible

05/03/2023

13

CONTINUE… Bioremediation of polluted site with

Chromate or dichromate (CrIV) is possible by stimulating the reduction of CrIV to CrIII by bacteria

Fungi can convert oxidized selenium to volatile-methylated selenides (vapour form) process named as volatilization

05/03/2023

14

MINERALIZATION

Examples of inorganic substances include all metals, ammonia, hydrogen sulfide, chlorine, salt, minerals such as gypsum and generally all compounds that do not contain carbon-hydrogen bonds.

05/03/2023

15

FAMOUS COMPANY CURRENTLY WORKING

Gencor in South Africa BioNIC process - Nickle extraction From low grade sulfide ore

Western Australia - Project in Uganda Cobalt from Pyrite ore

05/03/2023

16

RESEARCH Penicillium funiculosum works to extract 50%Ni 75%Zn from test solution containg 100mg/litre of metal at 6.6pH and 6.5pH respectively

Algal biosorbant of Chlorella vulgaris and Bacillus biomass used for treat waste water and metal recovery

05/03/2023

17

FUTURE PROSPECTSPositive points Effective Ecofriendly Economical

Good and effective metal biosorbents can replace commercial ion exchange resins

05/03/2023

18

BIOSORBANTS Certain natural materials of biological origin The biosorbent, unlike mono functional ion

exchange resins, contains variety of functional sites including carboxyl, imidazole, sulphydryl, amino, phosphate, sulfate, thioether, phenol, carbonyl, amide and hydroxyl moieties.

05/03/2023

19

CONTINUE…

Interdisciplinary approach between biotechnology and metallurgy will bring lab scale bioremediation to land scale technology

05/03/2023

20

Thank you