The Cromogenic Effect of Congo red Dye and its Impact in

Fungal CulturesSREEREMYA.S

ASSISTANT PROFESSORDEPT OF BIOTECHNOLOGY

SREE NARAYANA GURU COLLEGE,CBE

• Congo red dye is majorly used in histological staining and it has a major role to play in microbial screening.

• More significant use of congo red lies in microbial screening of cellulose predominantly carboxymethyl

• cellulose.

• Congo red is a dipolar molecular (Lorenc-Grabowska, E.et.al. 2007). It exists as anionic form at basic pH and as

• cationic form at acidic pH. Congo red dye has a significant role from the past history to till date (Banat et al.,

• 1996). Due to a color change from blue to red at pH 3.0–5.2, Congo red can be used as a pH indicator (Himmel

• ME.et.al.,1994).

• Congo red has a propensity to aggregate in aqueous and organic solutions (Miller E, 1968). The

• proposed mechanisms suggest hydrophobic interactions between the aromatic rings of the dye molecules,

• leading to a pi–pi stacking phenomenon

• SCREENING

• The fungal isolates were inoculated by spot inoculation on carboxymethyl cellulose agar plate and incubated at

• 24-48 hrs. 0.1% of congo red solution was added to the spot inoculated plates and then excess stains were

• removed by using 1M NaCl and zone of clearance were observed

• The result showed that among the eight fungal isolates isolated from soil, Six isolates showed cromogenic

• effect, among the six isolates two majorly showed change in coloration followed by minor zone of clearance. In

• some studies certain isolates majorly Aspergillusniger produced complete zone of clearance (Suther.I.W,

• 1999).Some of the isolates showed complete zone of clearance

REFERNCE

• The Cromogenic Effect of Congo red Dye in

• Fungal Cultures,S. Sreeremya, International Journal of Advance Research and Development.(Volume1, Issue1)