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Mesenchymal stem cellsDriving your MSC research
Human samples Mouse samples
Phenotyping of expanded MSCs
Human• MSCPhenotypingKit• Fluorochrome-conjugatedantibodiesagainst,e.g.,CD73,CD90,CD105,CD45,CD34,CD14,CD20,etc.
Mouse• Fluorochrome-conjugatedantibodiesagainst,e.g.,Lineagemarkers,CD105,CD117,Anti-Sca-1,CD45,CD116,etc.
• MACSQuant®FlowCytometers
Molecular analysis of MSCs
• mRNAIsolationKitandcDNASynthesisKit(forRT-PCR)• ProteinIsolationKits(forChIPornormalIPs)• Microarrayservices• SuperAmp™Technology• Bioinformatics• MultiMACS™M96Separator• Mitochondriaisolation
Differentiation of MSCs
Human• NHDifferentiationMedia• Cytokines,e.g.,TGF-β• NHCFU-F
Mouse• Cytokines,e.g.,FGF-8b
Characterization of MSCs
Sample type
Frozen• DeadCellRemovalKit• Pre-SeparationFilters
Tissues• gentleMACS™Dissociator• Pre-SeparationFilters
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated antibodies against, e.g.,• CD271• Anti-MSCA-1
• CD146• CD105• CD45
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated antibodies against, e.g.,• CD45
• Anti-Ter-119• Anti-Sca-1• CD105
Isolation of MSCs
Magnetic isolation• CD271(LNGFR)MicroBeadKits• Anti-MSCA-1(W8B2)MicroBeadKit
• CD90MicroBeads• CD105MicroBeads• CD146MicroBeadKit• CD73Antibodies• LineageCellDepletionKit
Flow sorting• Fluorochrome-conjugatedantibodies(markersaslisted)
Plastic adherence• NHExpansionMedia• CytoMix–MSC
Expansion of MSCs
• NHExpansionMedia
• CytoMix–MSC
• Cytokines,e.g.,FGF-2,PDGF-BB,TGF-β
Isolation of MSCs
Magnetic isolation• LineageCellDepletionKit• CD105MultiSortKit(PE),CD117MicroBeads,Anti-Sca-1MicroBeadKit(FITC)
• CD45MicroBeads,Anti-Ter-119MicroBeadsremovalpre-culture
• CD11bMicroBeadsremovalpost-culture
Flow sorting• Fluorochrome-conjugatedantibodies(markersaslisted)
Plastic adherence
Expansion of MSCs
• Cytokines,e.g.,TGF-β,EGF
Translate research to clinical applications• GMPCytokines• CliniMACS®System• CliniMACSReagents,e.g.,CD271
Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Clinicalapplications
Quick
guide
inside!
MACS® Stem Cell Research
macs-stemcells.com
Germany/Austria/SwitzerlandMiltenyiBiotecGmbHFriedrich-Ebert-Straße6851429BergischGladbachGermanyPhone+4922048306-0Fax+49220485197macs@miltenyibiotec.de
USA/CanadaMiltenyiBiotecInc.2303LindberghStreetAuburn,CA95602,USAPhone800FORMACSPhone+15308888871Fax+15308888925macs@miltenyibiotec.com
www.miltenyibiotec.com
Australia MiltenyiBiotecAustraliaPty.Ltd.Unit16A,2EdenParkDriveNorthRyde,NSW2113,AustraliaPhone+61288777400Fax+61298895044macs@miltenyibiotec.com.au
BeneluxMiltenyiBiotecB.V.Schipholweg68H2316XELeidenTheNetherlandsmacs@miltenyibiotec.nlCustomer service: The Netherlands Phone08004020120Fax08004020100Belgium Phone080094016Fax080099626Luxembourg Phone80024971Fax80024984
ChinaMiltenyiBiotecGmbHShanghaiOfficeRm.2309No.319XianxiaRd.Shanghai200051,P.R.ChinaPhone+862162351005Fax+862162350953macs@miltenyibiotec.com.cn
FranceMiltenyiBiotecSAS10rueMercoeur75011Paris,[email protected]
ItalyMiltenyiBiotecS.r.l.ViaPersicetana,2/D40012CalderaradiReno(BO)[email protected]
JapanMiltenyiBiotecK.K.Nittsu-EitaiBuilding5F16-10Fuyuki,Koto-ku,Tokyo135-0041,[email protected]
Nordics and BalticsMiltenyiBiotecNordenABScheelevä[email protected] service: Sweden Phone0200-111800Fax046-2807299 Denmark Phone80203010Fax+46462807299 Norway, Finland, Iceland, and Baltic countries Phone+46462807280Fax+46462807299
SingaporeMiltenyiBiotecAsiaPacificPteLtd.100BeachRoad#28-06to28-08ShawTowerSingapore189702Phone+6562388183Fax+6562380302macs@miltenyibiotec.com.sg
SpainMiltenyiBiotecS.L.C/LuisBuñuel2CiudaddelaImagen28223PozuelodeAlarcón(Madrid)[email protected]
United Kingdom MiltenyiBiotecLtd.AlmacHouse,ChurchLaneBisley,SurreyGU249DR,[email protected]
130-09
7-90
1.02
MiltenyiBiotecprovidesproductsandservicesworldwide.Visitwww.miltenyibiotec.com/localtofindyournearestMiltenyiBioteccontact.
Unlessotherwisespecificallyindicated,MiltenyiBiotecproductsandservicesareforresearchuseonlyandnotfortherapeuticordiagnosticuse.MACS,MACSelect,MACSQuant,CliniMACS,gentleMACS,MultiMACS,andSuperAmpareeitherregisteredtrademarksortrademarksofMiltenyiBiotecGmbH.CryoStem,Pluriton,StainAlive,andStemfectarealltrademarksofStemgent,Inc.Copyright©2013MiltenyiBiotecGmbH.Allrightsreserved.
Mesenchymal stem cellsDriving your MSC research
Human samples Mouse samples
Phenotyping of expanded MSCs
Human• MSCPhenotypingKit• Fluorochrome-conjugatedantibodiesagainst,e.g.,CD73,CD90,CD105,CD45,CD34,CD14,CD20,etc.
Mouse• Fluorochrome-conjugatedantibodiesagainst,e.g.,Lineagemarkers,CD105,CD117,Anti-Sca-1,CD45,CD116,etc.
• MACSQuant®FlowCytometers
Molecular analysis of MSCs
• mRNAIsolationKitandcDNASynthesisKit(forRT-PCR)• ProteinIsolationKits(forChIPornormalIPs)• Microarrayservices• SuperAmp™Technology• Bioinformatics• MultiMACS™M96Separator• Mitochondriaisolation
Differentiation of MSCs
Human• NHDifferentiationMedia• Cytokines,e.g.,TGF-β• NHCFU-F
Mouse• Cytokines,e.g.,FGF-8b
Characterization of MSCs
Sample type
Frozen• DeadCellRemovalKit• Pre-SeparationFilters
Tissues• gentleMACS™Dissociator• Pre-SeparationFilters
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated antibodies against, e.g.,• CD271• Anti-MSCA-1
• CD146• CD105• CD45
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated antibodies against, e.g.,• CD45
• Anti-Ter-119• Anti-Sca-1• CD105
Isolation of MSCs
Magnetic isolation• CD271(LNGFR)MicroBeadKits• Anti-MSCA-1(W8B2)MicroBeadKit
• CD90MicroBeads• CD105MicroBeads• CD146MicroBeadKit• CD73Antibodies• LineageCellDepletionKit
Flow sorting• Fluorochrome-conjugatedantibodies(markersaslisted)
Plastic adherence• NHExpansionMedia• CytoMix–MSC
Expansion of MSCs
• NHExpansionMedia
• CytoMix–MSC
• Cytokines,e.g.,FGF-2,PDGF-BB,TGF-β
Isolation of MSCs
Magnetic isolation• LineageCellDepletionKit• CD105MultiSortKit(PE),CD117MicroBeads,Anti-Sca-1MicroBeadKit(FITC)
• CD45MicroBeads,Anti-Ter-119MicroBeadsremovalpre-culture
• CD11bMicroBeadsremovalpost-culture
Flow sorting• Fluorochrome-conjugatedantibodies(markersaslisted)
Plastic adherence
Expansion of MSCs
• Cytokines,e.g.,TGF-β,EGF
Translate research to clinical applications• GMPCytokines• CliniMACS®System• CliniMACSReagents,e.g.,CD271
Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Clinicalapplications
Quick
guide
inside!
MACS® Stem Cell Research
macs-stemcells.com
Germany/Austria/SwitzerlandMiltenyiBiotecGmbHFriedrich-Ebert-Straße6851429BergischGladbachGermanyPhone+4922048306-0Fax+49220485197macs@miltenyibiotec.de
USA/CanadaMiltenyiBiotecInc.2303LindberghStreetAuburn,CA95602,USAPhone800FORMACSPhone+15308888871Fax+15308888925macs@miltenyibiotec.com
www.miltenyibiotec.com
Australia MiltenyiBiotecAustraliaPty.Ltd.Unit16A,2EdenParkDriveNorthRyde,NSW2113,AustraliaPhone+61288777400Fax+61298895044macs@miltenyibiotec.com.au
BeneluxMiltenyiBiotecB.V.Schipholweg68H2316XELeidenTheNetherlandsmacs@miltenyibiotec.nlCustomer service: The Netherlands Phone08004020120Fax08004020100Belgium Phone080094016Fax080099626Luxembourg Phone80024971Fax80024984
ChinaMiltenyiBiotecGmbHShanghaiOfficeRm.2309No.319XianxiaRd.Shanghai200051,P.R.ChinaPhone+862162351005Fax+862162350953macs@miltenyibiotec.com.cn
FranceMiltenyiBiotecSAS10rueMercoeur75011Paris,[email protected]
ItalyMiltenyiBiotecS.r.l.ViaPersicetana,2/D40012CalderaradiReno(BO)[email protected]
JapanMiltenyiBiotecK.K.Nittsu-EitaiBuilding5F16-10Fuyuki,Koto-ku,Tokyo135-0041,[email protected]
Nordics and BalticsMiltenyiBiotecNordenABScheelevä[email protected] service: Sweden Phone0200-111800Fax046-2807299 Denmark Phone80203010Fax+46462807299 Norway, Finland, Iceland, and Baltic countries Phone+46462807280Fax+46462807299
SingaporeMiltenyiBiotecAsiaPacificPteLtd.100BeachRoad#28-06to28-08ShawTowerSingapore189702Phone+6562388183Fax+6562380302macs@miltenyibiotec.com.sg
SpainMiltenyiBiotecS.L.C/LuisBuñuel2CiudaddelaImagen28223PozuelodeAlarcón(Madrid)[email protected]
United Kingdom MiltenyiBiotecLtd.AlmacHouse,ChurchLaneBisley,SurreyGU249DR,[email protected]
130-09
7-90
1.02
MiltenyiBiotecprovidesproductsandservicesworldwide.Visitwww.miltenyibiotec.com/localtofindyournearestMiltenyiBioteccontact.
Unlessotherwisespecificallyindicated,MiltenyiBiotecproductsandservicesareforresearchuseonlyandnotfortherapeuticordiagnosticuse.MACS,MACSelect,MACSQuant,CliniMACS,gentleMACS,MultiMACS,andSuperAmpareeitherregisteredtrademarksortrademarksofMiltenyiBiotecGmbH.CryoStem,Pluriton,StainAlive,andStemfectarealltrademarksofStemgent,Inc.Copyright©2013MiltenyiBiotecGmbH.Allrightsreserved.
Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Clinicalapplications Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Reprogramming
Embryonic and induced pluripotent stem cellsPioneering solutions for ES and iPS cells research
Hematopoietic stem cellsMaking headway in your HSC research
iPS cells
Do you differentiate your ES or iPS cells?
Characterization of reprogrammed cells
Human• ExtracellularPSCstaining:TRA-1-60,TRA-1-81,SSEA-3,SSEA-4,CD326,CD324,etc.
• Extracellulardifferentiatedcellstaining:SSEA-1
• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.
• MACSQuant®FlowCytometers
Mouse• ExtracellularPSCstaining:SSEA-1• Extracellulardifferentiatedcellstaining:Anti-Feederantibodies
• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.
• MACSQuantFlowCytometers
Isolation of reprogrammed cells
Magnetic isolationHuman• Anti-TRA-1-60MicroBeadKit• PluripotentStemCellMicroBeads• Anti-SSEA-4MicroBeads• IsolationofTRA-1-81+cellsviaindirectmagneticlabeling
• hESCloningandRecoverySupplement
Mouse• Anti-SSEA-1MicroBeads
Flow sorting• Fluorochrome-conjugatedantibodies(markersabove)
Colony Picking• StainAlive™Antibodies
Generation of iPS cells
Human• mRNAReprogrammingReagents• Pluriton™ReprogrammingMedia• Smallmolecules
Mouse• ReprogrammingEcotropicRetrovirus• SmallMolecules
Differentiation of ES or iPS cells
Human or mouse• Cytokines• Smallmolecules
Note:Differentcytokinesareusedinaccordancewiththedifferentiationpathway.
Purification of ES or iPS cells prior to differentiation
Human• Anti-TRA-1-60MicroBeadKit• PluripotentStemCellMicroBeads
Mouse• PluripotentStemCellIsolationKit
Differentiation of ES or iPS cells via embryoid bodies
• EmbryoidBodyDissociationKit• gentleMACS™Dissociator
Monitoring differentiation by flow cytometry or immunohistochemistry
• Antibodies,forexample,useCD34antibodiestoanalyzecellsduringdifferentiationintohematopoieticorendotheliallineages.
• MACSQuantFlowCytometers
Purification of cells during or after differentiation
MicroBeadreagents,forexample,cellseparationreagentsfordifferentiatedcells,e.g.,• NeuralCrestStemCellMicroBeads• CD34MicroBeads• CD144(VE-Cadherin)MicroBeads• CD309(Flk-1)MicroBeadKit• Anti-PSA-NCAMMicroBeadKit• Anti-A2B5MicroBeadKit
Human samples Mouse samples
Sample type
Frozen• DeadCellRemovalKit• Pre-SeparationFilters
PBMNCs or lysed whole blood• RedBloodCellLysisSolution
Isolation of HSCs
Magnetic isolation• LineageCellDepletionKit +CD117MicroBeads +Anti-Sca-1MicroBeadKit
(FITC)• CD105MultisortKit(PE)+Anti-Sca-1MicroBeadKit(FITC)
Flow sortingFluorochrome-conjugatedantibodiesagainst,e.g.,• Lineagemarkers• Anti-Sca-1• CD117(c-kit)• CD135(Flt3R)Isolation of HSCs
Magnetic isolation• CD34MicroBeadKit• CD133MicroBeadKit• LineageCellDepletionKit• CD117MicroBeadKit
Flow sortingFluorochrome-conjugatedantibodiesagainst,e.g.,• CD34• CD133• CD45• CD90• CD38
Expansion of HSCs
• Cytokines,e.g.,TPO,SCF,Flt3
Expansion of HSCs
• Cytokines,e.g.,SCF,Flt3
Phenotyping of HSCs
HumanFluorochrome-conjugatedantibodiesagainst,e.g.,• CD34• CD133• CD45• CD90• CD38• MACSQuant®FlowCytometers
MouseFluorochrome-conjugatedantibodiesagainst,e.g.,• Lineagemarkers• Sca-1• CD117(c-kit)• CD135(Flt3R)• MACSQuant FlowCytometers
Molecular analysis of HSCs
• mRNAIsolationKitandcDNASynthesisKit(forRT-PCR)
• ProteinIsolationKits(forChIPornormalIPs)
• Microarrayservices• SuperAmp™Technology• Bioinformatics• MultiMACS™M96Separator• Mitochondriaisolation
CFU assays for HSCs
Human• HSC-CFUMedia
Differentiation of HSCs
Human or mouse• Cytokines
Note:Differentcytokinesareusedinaccordancewiththedifferentiationpathway.
Characterization of HSCs
ES cells
Transfection of ES or iPS cells
Human• Stemfect™hESCDNATransfectionKit• StemfectEnhancedhESCDNATransfectionKit• StemfectRNATransfectionKit• MACSelect™Products
Mouse• Stemfect2.0-mESCDNATransfectionReagent• MACSelect™Products
Single-cell passaging of ES or iPS cells
Human• hESCloningandRecoverySupplement• Anti-TRA-1-60MicroBeadKit• PluripotentStemCellMicroBeads
Monitoring of pluripotency by flow cytometry or immunohistochemistry
Human• ExtracellularPSCstaining:TRA-1-60,TRA-1-81,SSEA-3,SSEA-4,CD326,CD324,etc.• Extracellulardifferentiatedcellstaining:SSEA-1• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.• MACSQuant®FlowCytometers
Mouse• ExtracellularPSCstaining:SSEA-1• Extracellulardifferentiatedcellstaining:Anti-Feederantibodies• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.• MACSQuant®FlowCytometers
Translate research to clinical applications• GMPCytokines• CliniMACSSystem• CliniMACS®Reagents,e.g.,CD34,CD133
Removal of feeder cells
Depleting feeder cellsHuman• Anti-FibroblastMicroBeads
Mouse• FeederRemovalMicroBeads
Isolating pluripotent stem cellsHuman• Anti-TRA-1-60MicroBeadKit
• PluripotentStemCellMicroBeads
Mouse• Anti-SSEA-1MicroBeads
Culture of ES or iPS cells
Feeder-basedHuman• Cytokines,e.g.,FGF2• CryoStem™FreezingMedium• Smallmolecules
Mouse• Cytokines,e.g.,LIF• Smallmolecules
Feeder-freeHuman• NutriStem™Medium• Cytokines,e.g.,FGF2• Smallmolecules• CryoStemFreezingMedium
Mouse• Cytokines,e.g.,LIF• Smallmolecules
Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Clinicalapplications Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Reprogramming
Embryonic and induced pluripotent stem cellsPioneering solutions for ES and iPS cells research
Hematopoietic stem cellsMaking headway in your HSC research
iPS cells
Do you differentiate your ES or iPS cells?
Characterization of reprogrammed cells
Human• ExtracellularPSCstaining:TRA-1-60,TRA-1-81,SSEA-3,SSEA-4,CD326,CD324,etc.
• Extracellulardifferentiatedcellstaining:SSEA-1
• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.
• MACSQuant®FlowCytometers
Mouse• ExtracellularPSCstaining:SSEA-1• Extracellulardifferentiatedcellstaining:Anti-Feederantibodies
• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.
• MACSQuantFlowCytometers
Isolation of reprogrammed cells
Magnetic isolationHuman• Anti-TRA-1-60MicroBeadKit• PluripotentStemCellMicroBeads• Anti-SSEA-4MicroBeads• IsolationofTRA-1-81+cellsviaindirectmagneticlabeling
• hESCloningandRecoverySupplement
Mouse• Anti-SSEA-1MicroBeads
Flow sorting• Fluorochrome-conjugatedantibodies(markersabove)
Colony Picking• StainAlive™Antibodies
Generation of iPS cells
Human• mRNAReprogrammingReagents• Pluriton™ReprogrammingMedia• Smallmolecules
Mouse• ReprogrammingEcotropicRetrovirus• SmallMolecules
Differentiation of ES or iPS cells
Human or mouse• Cytokines• Smallmolecules
Note:Differentcytokinesareusedinaccordancewiththedifferentiationpathway.
Purification of ES or iPS cells prior to differentiation
Human• Anti-TRA-1-60MicroBeadKit• PluripotentStemCellMicroBeads
Mouse• PluripotentStemCellIsolationKit
Differentiation of ES or iPS cells via embryoid bodies
• EmbryoidBodyDissociationKit• gentleMACS™Dissociator
Monitoring differentiation by flow cytometry or immunohistochemistry
• Antibodies,forexample,useCD34antibodiestoanalyzecellsduringdifferentiationintohematopoieticorendotheliallineages.
• MACSQuantFlowCytometers
Purification of cells during or after differentiation
MicroBeadreagents,forexample,cellseparationreagentsfordifferentiatedcells,e.g.,• NeuralCrestStemCellMicroBeads• CD34MicroBeads• CD144(VE-Cadherin)MicroBeads• CD309(Flk-1)MicroBeadKit• Anti-PSA-NCAMMicroBeadKit• Anti-A2B5MicroBeadKit
Human samples Mouse samples
Sample type
Frozen• DeadCellRemovalKit• Pre-SeparationFilters
PBMNCs or lysed whole blood• RedBloodCellLysisSolution
Isolation of HSCs
Magnetic isolation• LineageCellDepletionKit +CD117MicroBeads +Anti-Sca-1MicroBeadKit
(FITC)• CD105MultisortKit(PE)+Anti-Sca-1MicroBeadKit(FITC)
Flow sortingFluorochrome-conjugatedantibodiesagainst,e.g.,• Lineagemarkers• Anti-Sca-1• CD117(c-kit)• CD135(Flt3R)Isolation of HSCs
Magnetic isolation• CD34MicroBeadKit• CD133MicroBeadKit• LineageCellDepletionKit• CD117MicroBeadKit
Flow sortingFluorochrome-conjugatedantibodiesagainst,e.g.,• CD34• CD133• CD45• CD90• CD38
Expansion of HSCs
• Cytokines,e.g.,TPO,SCF,Flt3
Expansion of HSCs
• Cytokines,e.g.,SCF,Flt3
Phenotyping of HSCs
HumanFluorochrome-conjugatedantibodiesagainst,e.g.,• CD34• CD133• CD45• CD90• CD38• MACSQuant®FlowCytometers
MouseFluorochrome-conjugatedantibodiesagainst,e.g.,• Lineagemarkers• Sca-1• CD117(c-kit)• CD135(Flt3R)• MACSQuant FlowCytometers
Molecular analysis of HSCs
• mRNAIsolationKitandcDNASynthesisKit(forRT-PCR)
• ProteinIsolationKits(forChIPornormalIPs)
• Microarrayservices• SuperAmp™Technology• Bioinformatics• MultiMACS™M96Separator• Mitochondriaisolation
CFU assays for HSCs
Human• HSC-CFUMedia
Differentiation of HSCs
Human or mouse• Cytokines
Note:Differentcytokinesareusedinaccordancewiththedifferentiationpathway.
Characterization of HSCs
ES cells
Transfection of ES or iPS cells
Human• Stemfect™hESCDNATransfectionKit• StemfectEnhancedhESCDNATransfectionKit• StemfectRNATransfectionKit• MACSelect™Products
Mouse• Stemfect2.0-mESCDNATransfectionReagent• MACSelect™Products
Single-cell passaging of ES or iPS cells
Human• hESCloningandRecoverySupplement• Anti-TRA-1-60MicroBeadKit• PluripotentStemCellMicroBeads
Monitoring of pluripotency by flow cytometry or immunohistochemistry
Human• ExtracellularPSCstaining:TRA-1-60,TRA-1-81,SSEA-3,SSEA-4,CD326,CD324,etc.• Extracellulardifferentiatedcellstaining:SSEA-1• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.• MACSQuant®FlowCytometers
Mouse• ExtracellularPSCstaining:SSEA-1• Extracellulardifferentiatedcellstaining:Anti-Feederantibodies• Transcriptionfactorstaining:Oct4,Sox2,Nanog,etc.• MACSQuant®FlowCytometers
Translate research to clinical applications• GMPCytokines• CliniMACSSystem• CliniMACS®Reagents,e.g.,CD34,CD133
Removal of feeder cells
Depleting feeder cellsHuman• Anti-FibroblastMicroBeads
Mouse• FeederRemovalMicroBeads
Isolating pluripotent stem cellsHuman• Anti-TRA-1-60MicroBeadKit
• PluripotentStemCellMicroBeads
Mouse• Anti-SSEA-1MicroBeads
Culture of ES or iPS cells
Feeder-basedHuman• Cytokines,e.g.,FGF2• CryoStem™FreezingMedium• Smallmolecules
Mouse• Cytokines,e.g.,LIF• Smallmolecules
Feeder-freeHuman• NutriStem™Medium• Cytokines,e.g.,FGF2• Smallmolecules• CryoStemFreezingMedium
Mouse• Cytokines,e.g.,LIF• Smallmolecules
Mesenchymal stem cellsDriving your MSC research
Human samples Mouse samples
Phenotyping of expanded MSCs
Human• MSCPhenotypingKit• Fluorochrome-conjugatedantibodiesagainst,e.g.,CD73,CD90,CD105,CD45,CD34,CD14,CD20,etc.
Mouse• Fluorochrome-conjugatedantibodiesagainst,e.g.,Lineagemarkers,CD105,CD117,Anti-Sca-1,CD45,CD116,etc.
• MACSQuant®FlowCytometers
Molecular analysis of MSCs
• mRNAIsolationKitandcDNASynthesisKit(forRT-PCR)• ProteinIsolationKits(forChIPornormalIPs)• Microarrayservices• SuperAmp™Technology• Bioinformatics• MultiMACS™M96Separator• Mitochondriaisolation
Differentiation of MSCs
Human• NHDifferentiationMedia• Cytokines,e.g.,TGF-β• NHCFU-F
Mouse• Cytokines,e.g.,FGF-8b
Characterization of MSCs
Sample type
Frozen• DeadCellRemovalKit• Pre-SeparationFilters
Tissues• gentleMACS™Dissociator• Pre-SeparationFilters
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated antibodies against, e.g.,• CD271• Anti-MSCA-1
• CD146• CD105• CD45
Phenotyping of MSCs prior to expansion
Fluorochrome-conjugated antibodies against, e.g.,• CD45
• Anti-Ter-119• Anti-Sca-1• CD105
Isolation of MSCs
Magnetic isolation• CD271(LNGFR)MicroBeadKits• Anti-MSCA-1(W8B2)MicroBeadKit
• CD90MicroBeads• CD105MicroBeads• CD146MicroBeadKit• CD73Antibodies• LineageCellDepletionKit
Flow sorting• Fluorochrome-conjugatedantibodies(markersaslisted)
Plastic adherence• NHExpansionMedia• CytoMix–MSC
Expansion of MSCs
• NHExpansionMedia
• CytoMix–MSC
• Cytokines,e.g.,FGF-2,PDGF-BB,TGF-β
Isolation of MSCs
Magnetic isolation• LineageCellDepletionKit• CD105MultiSortKit(PE),CD117MicroBeads,Anti-Sca-1MicroBeadKit(FITC)
• CD45MicroBeads,Anti-Ter-119MicroBeadsremovalpre-culture
• CD11bMicroBeadsremovalpost-culture
Flow sorting• Fluorochrome-conjugatedantibodies(markersaslisted)
Plastic adherence
Expansion of MSCs
• Cytokines,e.g.,TGF-β,EGF
Translate research to clinical applications• GMPCytokines• CliniMACS®System• CliniMACSReagents,e.g.,CD271
Samplepreparation Cellseparation Cellanalysis Cellculture Molecularapplications Clinicalapplications
Quick
guide
inside!
MACS® Stem Cell Research
macs-stemcells.com
Germany/Austria/SwitzerlandMiltenyiBiotecGmbHFriedrich-Ebert-Straße6851429BergischGladbachGermanyPhone+4922048306-0Fax+49220485197macs@miltenyibiotec.de
USA/CanadaMiltenyiBiotecInc.2303LindberghStreetAuburn,CA95602,USAPhone800FORMACSPhone+15308888871Fax+15308888925macs@miltenyibiotec.com
www.miltenyibiotec.com
Australia MiltenyiBiotecAustraliaPty.Ltd.Unit16A,2EdenParkDriveNorthRyde,NSW2113,AustraliaPhone+61288777400Fax+61298895044macs@miltenyibiotec.com.au
BeneluxMiltenyiBiotecB.V.Schipholweg68H2316XELeidenTheNetherlandsmacs@miltenyibiotec.nlCustomer service: The Netherlands Phone08004020120Fax08004020100Belgium Phone080094016Fax080099626Luxembourg Phone80024971Fax80024984
ChinaMiltenyiBiotecGmbHShanghaiOfficeRm.2309No.319XianxiaRd.Shanghai200051,P.R.ChinaPhone+862162351005Fax+862162350953macs@miltenyibiotec.com.cn
FranceMiltenyiBiotecSAS10rueMercoeur75011Paris,[email protected]
ItalyMiltenyiBiotecS.r.l.ViaPersicetana,2/D40012CalderaradiReno(BO)[email protected]
JapanMiltenyiBiotecK.K.Nittsu-EitaiBuilding5F16-10Fuyuki,Koto-ku,Tokyo135-0041,[email protected]
Nordics and BalticsMiltenyiBiotecNordenABScheelevä[email protected] service: Sweden Phone0200-111800Fax046-2807299 Denmark Phone80203010Fax+46462807299 Norway, Finland, Iceland, and Baltic countries Phone+46462807280Fax+46462807299
SingaporeMiltenyiBiotecAsiaPacificPteLtd.100BeachRoad#28-06to28-08ShawTowerSingapore189702Phone+6562388183Fax+6562380302macs@miltenyibiotec.com.sg
SpainMiltenyiBiotecS.L.C/LuisBuñuel2CiudaddelaImagen28223PozuelodeAlarcón(Madrid)[email protected]
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