Preparation of Blood Smear and Gross Examination

By: Shefaa Hejazy

Slide to slide Method

Spin Method

• Using a special centrifuge (e.g. Cytospin); the blood spreads on the slide in a mono-layer.

Staining • Romanowsky Stains: Leishman’s Stain May-Grunwald-Giemsa Stain Jenner’s Stain Wright’s Stain

• Principle? Acidic elements in the cell (e.g. nucleus proteins and nucleic acids of cell nucleus) + Basic dye (Azure-B)

Basic elements such as (Hb) + Acidic dye (Eosin-Y)

Blue

Red

• Requirements: Leishman’s Stain Leishman’s Buffer solution Staining Rack Methanol (fixation) D.W. Pipette and cotton or gauze

Staining

Sub-tail

Thank You