Bacteria based micro robot used in cancer treatment

Korean research team has successfully developed First Cancer-treating Nanorobot

http://www.youtube.com/watch?v=VYwLy2Jb8Ik

Introduction

• Cancer is the disease caused by an uncontrolled division of abnormal cells in a part of the body

• Chemotherapy , is the conventional treatment but..

Damage growing-healthy normal cells blood and hair cells

Not specific and No continuous drug delivery for tissues

Negative side effects ( anemia , nausea )

Nano robots in medicine

• This research offer an alternative way instead of chemotherapy

• Bacteria based micro robot ( Bacteriobot )

• Bacteriobot = genetically engineered Bacteria + micro structure bead ( drug )

Why Nano robots ?

• Specific .. target cancer cells but not normal cells

• Fast .. one day make sense with cancer treatment

• Depends on biodegradable and biocompatible materials

• optimal drug quantities delivered to the target cells

How bacteriobot works?

• To be effective , bacteriobot moves toward tumors acting as combination of

• Micro sensor

• Micro actuator

• Therapeutic agent

Designing the experiment

• Tumor model : colorectal cancer , breast cancer 4T1

• Animal model : CT_26 mouse

• Bacteria model : ppGpp S. typhimurium strain SHJ2037

Making the bacteriobot

How to combine Salmonella to capsule ?

• To deliver drug to tumor cells

• Salmonella work as mechanical engine

• Micro capsule work as drug container

Engineering the bacteriobot

• It is a fusion between RT* and BT* presents a novel anti tumor strategy

• Stage 1 : Interaction between biotin – streptavidin

• Biotin found in all living organisms

• Attenuated salmonella genetically modified to express biotin on the outer membrane of the cell

* RT : Robot technology

* BT : Biotechnology

• High affinity interaction between biotin – streptavidin

• Biotin can conjugate to many proteins easily let that protein to interact to with streptavidin conjugated molecules

• Stage 2 : Development of flow-free chemotactic microfluidic chamber

• to evaluate targeting , motility , velocity of bacteriobot

• This device composed of 3 chambers :

Normal cells - Bacteriobots - tumor cells

• Uses fluorescent dyes to see Nano robots target cells

flow-free chemotactic microfluidic chamber

• Stage 3 : Tumor targeting and localization of bacteriobot

• It is the chemotactic response of bacteriobot to the [con] gradients of various of cancer cells :

• NIH/3T3 , CT_26 ( colorectal cancer )

• 4T1 ( breast cancer )

• Targeting measured by migration of bacteria out of central region toward the side chambers

Results

References

"New Paradigm for Tumor Theranostic Methodology Using Bacteria-based Microrobot."Nature.com. Nature Publishing Group, n.d. Web. 18

Feb. 2014.

Done by : Belal Abu Haneya

Hashemite University – Jordan

Supervisor : Dr. Abd Allatif Al Ghzawi