1. ENOS ELISA is an immunoassay kit that is used for
determining eNOS concentrations in various organisms. There are
various forms of this assay that can be variably used to determine
the eNOS concentrations in animals like mouse, pigs, bovine, etc.,
as well as humans. ENOS stands for endothelial nitric oxide
synthase and is an enzyme that is encoded by the NOS3 gene in case
of humans. Nitric oxide synthase belongs to that family of enzymes
that catalyzes the production of nitric oxide from L-Arginine, an
amino acid. A variation of this endothelial nitric oxide synthase
gene gives it a significant role in various kinds of research and
clinical tests. Following are the areas of application and their
implications where ENOS ELISA is used:
2. This is for the quantitative determination of human eNOS
concentrations from the endothelial cells by applying the sandwich
ELISA method. In a Microtiter plate that has been pre-coated with
antibody-specific eNOS, standards and samples are added. They are
pipetted into the wells, and the residual NOS3 gets bounded by the
immobilized antibody. Following that, a polyclonal antibody
specific for endothelial nitric oxide synthase is added to the
wells. Later, a TMB substrate solution is added to each wells. This
will lead to a color development in both that contains eNOS. The
enzyme-substrate reaction, or in other words the color development
process is stopped by adding sulfuric acid solutions after which,
the intensity of the color change is measured.
3. In this case, the immunoassay kit allows for quantitative
measurement of Porcine eNOS concentrations in cell, plasma, serum,
and cell lysates. The assay is similar to the one that is used in
case of humans. Only, the concentration of eNOS in the sample is
calculated by comparing the O.D. value of the sample to the
standard curve. This enzyme-linked immunoabsorbent assay kit is
meant for in vitro specific measurement of mouse endothelial nitric
oxide synthase or eNOS concentration in serums, plasma, tissue
homogenates, and other biological fluids. While the entire
procedure is similar to the other assay processes, the sensitivity
of this assay is pointed as the lowest protein concentration that
can be differentiated from zero. In case of mouse, the minimum dose
of eNOS is less than 0.087ng/ml.
4. Used largely for the same purpose, the lowest detectable
dose of rat eNOS is 0.78 U/L. An effective storage facility for the
kit is highly essential for a perfect experiment. It should be kept
in mind that the kit must be stored at -20 degrees centigrade.
Unused strips should be kept in airtight bags with temperatures
maintained between 2-8 degree Celsius. The kit is also used in the
quantitative determination of enzymes in other animals. You can
easily find the kit online. There are various authentic online
sources that deal with the supply of these products and other
related immunoassay kits. Get it in their best condition and make
your experiment successful and result-specific.
5. EIAab is a provider of high-quality protein related products
that comes into application in various scientific research
purposes. Find the best quality of ENOS ELISA, and other assay kits
from the site.
