Scheme carbohydratesUNKNOWN CARBOHYDRATE SOLUTION

Dr Rohini C Sane

Professor

Department of Biochemistry

DY PATIL MEDICAL COLLEGE ,EBENE

UNKNOWN CARBOHYDRATE SOLUTION

1.BENEDICT’S TEST

POSITIVE NEGATIVE

REDUCING CARBOHYDRATE NON REDUCING CARBOHYDRATE GLUCOSE, FRUCTOSE,LACTOSE ,MALTOSE SUCROSE

2. BARFOED’S TEST 2. BARFOED’S TEST

POSITIVE NEGATIVE NEGATIVEMONOSACCHARIDE DISACCHARIDE DISACCHARIDE

GLUCOSE, FRUCTOSE LACTOSE ,MALTOSE

3. SELIWANOFF TEST 3. SELIWANOFF TEST 3. SELIWANOFF TESTPOSITIVE NEGATIVE NEGATIVE POSITIVEFRUCTOSE GLUCOSE LACTOSE MALTOSE SUCROSE

OSAZONES –BUNDLES OF HAY LADY POWDER PUFF SUNFLOWER SHAPED CT INVERSION TEST

GLUCOSE FRUCTOSE LACTOSE MALTOSE SUCROSE

BENEDICT”S TEST

POSITIVE POSITIVE POSITIVE POSITIVE NEGATIVE

BARFOED’s TEST

POSITIVE POSITIVE NEGATIVE NEGATIVE NEGATIVE

SELIWANOFF ‘S TEST

NEGATIVE POSITIVE NEGATIVE NEGATIVE POSITIVE

OSAZONES HAY SHAPED (GRASS),GREEN

HAY SHAPED (GRASS),GREEN

LADY POWDER PUFF

SUNFLOWER SHAPED

NO OSAZONES

CONCLUSIONS REDUCING MONOSACCHARIDE ,ALDOSE

REDUCING MONOSACCHARIDE ,KETOSE

REDUCING DISACCHARIDE ,ALDOSE

REDUCING DISACCHARIDE ,ALDOSE

NON REDUCING DISACCHARIDE,KETOSE