Bill Andrews PhD

Discovered Human Telomerase

2nd Place United States Inventor of the Year

Telomere Biologist for 19 Years

31 Years in Biotech Research

43 US Issued Patents on Telomerase

Founder President amp CEO Sierra Sciences

Popular Science The Today Show etc

Short

Telomere

Disease

Bad Things Happen When

Telomeres Get Short Telomere

Telomere

Congratulations Elizabeth Blackburn Carol Greider and Jack Szostak

2009 Nobel Prize in Medicine

Some People are Born with Short

Telomeres

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Short

Telomere

Disease

Bad Things Happen When

Telomeres Get Short Telomere

Telomere

Congratulations Elizabeth Blackburn Carol Greider and Jack Szostak

2009 Nobel Prize in Medicine

Some People are Born with Short

Telomeres

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Bad Things Happen When

Telomeres Get Short Telomere

Telomere

Congratulations Elizabeth Blackburn Carol Greider and Jack Szostak

2009 Nobel Prize in Medicine

Some People are Born with Short

Telomeres

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Telomere

Congratulations Elizabeth Blackburn Carol Greider and Jack Szostak

2009 Nobel Prize in Medicine

Some People are Born with Short

Telomeres

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Congratulations Elizabeth Blackburn Carol Greider and Jack Szostak

2009 Nobel Prize in Medicine

Some People are Born with Short

Telomeres

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Some People are Born with Short

Telomeres

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Life Expectancy - 20 Years

Progeria

Mech Ageing Dev 2009 Jun130(6)377-83 Epub 2009 Mar 20

Short Telomere

Disease

Life Expectancy - 20 Years

Preventing Telomere Shortening

Could Cure These Kids

Bad Things Happen When

Telomeres Get Short

Normal Health amp Aging

Short Telomere Diseases

bull Cardiovascular

bull Cancer

bull COPD

bull Degenerative Disc Disease

bull Alzheimerrsquos

bull Osteoarthritis

bull Rheumatoid Arthritis

bull Osteoporosis

bull General Immunity

bull Skin Aging

bull Macular Degeneration

bull Liver Cirrhosis

bull Muscular Dystrophy

bull Cell amp Tissue Transplants

bull AIDS

bull Progeria

bull Dyskeratosis Congenita

bull Idiopathic Pulmonary Fibrosis

bull Cri du Chat syndrome

bull Downrsquos Syndrome

bull Fanconirsquos Anemia

bull Tuberous Sclerosis

bull Wernerrsquos Syndrome

bull And Aging Itself

Bad Things Happen When

Telomeres Get Short

Dr David

Woynarowski

1971 Nixon declares War on Cancer

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Normal Health amp Aging

Short Telomere Diseases

bull Cardiovascular

bull Cancer

bull COPD

bull Degenerative Disc Disease

bull Alzheimerrsquos

bull Osteoarthritis

bull Rheumatoid Arthritis

bull Osteoporosis

bull General Immunity

bull Skin Aging

bull Macular Degeneration

bull Liver Cirrhosis

bull Muscular Dystrophy

bull Cell amp Tissue Transplants

bull AIDS

bull Progeria

bull Dyskeratosis Congenita

bull Idiopathic Pulmonary Fibrosis

bull Cri du Chat syndrome

bull Downrsquos Syndrome

bull Fanconirsquos Anemia

bull Tuberous Sclerosis

bull Wernerrsquos Syndrome

bull And Aging Itself

Bad Things Happen When

Telomeres Get Short

Dr David

Woynarowski

1971 Nixon declares War on Cancer

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Short Telomere Diseases

bull Cardiovascular

bull Cancer

bull COPD

bull Degenerative Disc Disease

bull Alzheimerrsquos

bull Osteoarthritis

bull Rheumatoid Arthritis

bull Osteoporosis

bull General Immunity

bull Skin Aging

bull Macular Degeneration

bull Liver Cirrhosis

bull Muscular Dystrophy

bull Cell amp Tissue Transplants

bull AIDS

bull Progeria

bull Dyskeratosis Congenita

bull Idiopathic Pulmonary Fibrosis

bull Cri du Chat syndrome

bull Downrsquos Syndrome

bull Fanconirsquos Anemia

bull Tuberous Sclerosis

bull Wernerrsquos Syndrome

bull And Aging Itself

Bad Things Happen When

Telomeres Get Short

Dr David

Woynarowski

1971 Nixon declares War on Cancer

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Bad Things Happen When

Telomeres Get Short

Dr David

Woynarowski

1971 Nixon declares War on Cancer

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Dr David

Woynarowski

1971 Nixon declares War on Cancer

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

1971 Nixon declares War on Cancer

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

War on

Short Telomere

Disease

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

September 2-8

is

Telomere

Research

Week

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

What Can Be Done About

Telomere Shortening

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Managing Telomere Length

1 Know the length of your telomeres

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomere Testing

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth Normal Line

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

A Normal 50 Year Old

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomeric lt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomeric gt Chronological

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Accelerated Aging

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Progeria

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Always Hope to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Never Want to Be

0

2

4

6

8

10

0 20 40 60 80 100

Age (Years)

Telo

mere

Len

gth

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH

5 HT Q-FISH

6 Universal STELA

Short

Telomeres

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomere Length Measurement

1 Terminal Restriction Fragment (TRF)

2 Q-PCR ndash Commercially available

3 Fluorescence In Situ Hybridization (FISH)

4 Flow FISH ndash Commercially available

5 HT Q-FISH ndash Commercially available

6 Universal STELA

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomeres Shorten as Cells Divide

Hastings et al Cell Prolif 37 317-324 (2004)

Telomeres Shorten with Age

Tsuji et al Forensic Sci Int 2002

Okuda K et al Atherosclerosis 2000

Telomeres Shorten with Age

Weak Correlation

People born with different telomere lengths

Rates of cell division differ between people

Different environmental factors accelerate

telomere shortening

Poor Signal Detectability

Weak Correlation

Good for Population Studies

or

Looking at Major Differences

in Individuals

Abundance of Short Telomeres

Abundance of Short Telomeres

Before

Before

After

After

Small Differences

Easier to Detect

Managing Telomere Length

1 Know the length of your telomeres

2 Decrease the rate of telomere shortening

Exercise

Exercise

German National Track and Field Team

Werner et al Circulation 2009 Dec 15120(24)2438-47

p lt 0001

German National Track and Field Team

p lt 0001

Werner et al Circulation 2009 Dec 15120(24)2438-47

Vigorous Aerobic Exercise

LaRocca et al Mech Ageing Dev 2010 Feb131(2)165-7

Physical Activity in Leisure Time

Cherkas et al Arch Intern Med 2008 Jan 28168(2)154-8

Stress

Stress

p = 005

Damjanovic et al J Immunol 2007 Sep 15179(6)4249-54

Stress

Epel et al Proc Natl Acad Sci U S A 2004 Dec 7101(49)17312-5

Stress

Tyrka et al Biol Psychiatry 2010 Mar 5 [Epub ahead of print]

Depression

Mood Disorders

Simon et al Biol Psychiatry 2006 Sep 160(5)432-5

Obesity Diet

Antioxidants amp

Smoking

2284 women from the Nursesrsquo Health Study

Cassidy et al Am J Clin Nutr 2010 Mar 10 [Epub ahead of print]

Valdes et al Lancet 2005 Aug 20-26366(9486)662-4

p = 0031 p = 0019

p = 0017 p = 0045

Obesity

Smoking

Smoking

p = 001

Morlaacute et al Eur Respir J 2006 Mar27(3)525-8

Omega 3 Fatty Acids

Omega 3 Fatty Acids

Farzaneh-Far et al JAMA 2010 Jan 20303(3)250-7

Vitamin D

Vitamin D

Richards et al Am J Clin Nutr 2007 Nov86(5)1420-5

Attitude

The MONICA Northern Sweden Study

Self Perceived Aging

Nordfjaumlll et al Scand J Public Health 2008 Sep36(7)744-52

The MONICA Northern Sweden Study

Self Perceived Aging

Nordfjaumlll et al Scand J Public Health 2008 Sep36(7)744-52

The MONICA Northern Sweden Study

Self Perceived Aging

Nordfjaumlll et al Scand J Public Health 2008 Sep36(7)744-52

Pessimism

OrsquoDonovan et al Brain Behav Immun 2009 May23(4)446-9

Keeping Telomeres Long

Exercise

Anti-Oxidants

Omega 3rsquos

Vitamin D3

Donrsquot Smoke

Donrsquot be Obese

Reduce Stress

Reduce Depression

Reduce Pessimism

Be Happy

Managing Telomere Length

1 Know the length of your telomeres

2 Decrease the rate of telomere shortening

3 Increase the length of the telomeres

Telomerase

Telomere

Telomerase

Sierra Sciences LLC

ABC News

Diane Sawyer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

Managing Telomere Length

1 Know the length of your telomeres

2 Decrease the rate of telomere shortening

3 Increase the length of the telomeres

Telomerase

Telomere

Telomerase

Sierra Sciences LLC

ABC News

Diane Sawyer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

Telomerase

Telomere

Telomerase

Sierra Sciences LLC

ABC News

Diane Sawyer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

Sierra Sciences LLC

ABC News

Diane Sawyer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

ABC News

Diane Sawyer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

Bernardes de Jesus B Vera E Schneeberger K Tejera AM Ayuso E Bosch F Blasco MA EMBO Mol

Med 2012 Aug4(8)691-704 Epub 2012 May 15

Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer

Searching for Telomerase

Inducers

Reproductive Cells Produce Telomerase

Telomerase Gene Regulatory

Element

Reproductive Cells Produce Telomerase

Telomerase Gene Regulatory

Element

Telomerase Gene Regulatory

Element

The Gene is Repressed in All Other Cells

Sierra Sciences LLC

39 Families of Chemicals

Identified

Human Testing within 3 Years

Natural Products Already on the

Market

Clinical Studies

Study 1

Not Published

Study 2

Telomerase Induction In Vitro

Ch

ap

s

10

0

He

la

5

He

la

MR

C5

DM

SO

MR

C5

Me

dia

001uM 01uM 1uM 10uM 100uM

Abundance of Short Telomeres

Reduced

(Harley Liu Blasco Andrews Briggs and Raffaele Rejuvenation Research

2010)

Clinical Study

Telomerase Induction

Short Telomeres Lengthened

CD28- T Cells

Neutrophils

Natural Killer Cells

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Study 1

Not Published

Study 2

Telomerase Induction In Vitro

Ch

ap

s

10

0

He

la

5

He

la

MR

C5

DM

SO

MR

C5

Me

dia

001uM 01uM 1uM 10uM 100uM

Abundance of Short Telomeres

Reduced

(Harley Liu Blasco Andrews Briggs and Raffaele Rejuvenation Research

2010)

Clinical Study

Telomerase Induction

Short Telomeres Lengthened

CD28- T Cells

Neutrophils

Natural Killer Cells

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Study 2

Telomerase Induction In Vitro

Ch

ap

s

10

0

He

la

5

He

la

MR

C5

DM

SO

MR

C5

Me

dia

001uM 01uM 1uM 10uM 100uM

Abundance of Short Telomeres

Reduced

(Harley Liu Blasco Andrews Briggs and Raffaele Rejuvenation Research

2010)

Clinical Study

Telomerase Induction

Short Telomeres Lengthened

CD28- T Cells

Neutrophils

Natural Killer Cells

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Telomerase Induction In Vitro

Ch

ap

s

10

0

He

la

5

He

la

MR

C5

DM

SO

MR

C5

Me

dia

001uM 01uM 1uM 10uM 100uM

Abundance of Short Telomeres

Reduced

(Harley Liu Blasco Andrews Briggs and Raffaele Rejuvenation Research

2010)

Clinical Study

Telomerase Induction

Short Telomeres Lengthened

CD28- T Cells

Neutrophils

Natural Killer Cells

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Abundance of Short Telomeres

Reduced

(Harley Liu Blasco Andrews Briggs and Raffaele Rejuvenation Research

2010)

Clinical Study

Telomerase Induction

Short Telomeres Lengthened

CD28- T Cells

Neutrophils

Natural Killer Cells

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Clinical Study

Telomerase Induction

Short Telomeres Lengthened

CD28- T Cells

Neutrophils

Natural Killer Cells

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Study 3

Completed but Not Published Yet

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Clinical Study

Naive T Cells

Blood Pressure

Skin Elasticity

Bone Density

Cholesterol

Homocysteine

Cognitive Function

Glucose Levels

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Clinical Studies

Underway

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Study 4

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Clinical Study

Faces Hands etc Photographed

Short telomeres measured

Waist circumference

Body Mass Index (BMI)

Total Body Water (TBW)

Total Body Fat (TBF)

Oxidative Stress Panel

Routine Urinalysis

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Clinical Study

CBC wDiff

Thyroid panel including a Free T3

HSCRP (Highly sensitive C Reactive

Protein)

Total and free Testosterone

Total Estrogen

Chem 20 including a lipid profile

IGF-1

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Study 5

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Clinical Study

Double Blind Placebo Controlled

Short Telomeres Measured

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

The War has

Just Begun

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

Recent Publications

wwwsierrascicomRecPubshtml

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

wwwsierrascicomRecPubshtml

QuestionsForBillyahoocom

Thank you

QuestionsForBillyahoocom

Thank you