Upload
bill-marler
View
620
Download
1
Tags:
Embed Size (px)
DESCRIPTION
Foodborne illness attorney Bill Marler speaks to a Hyla Middle School Science about how food poisoning outbreaks are investigated
Citation preview
Making the Causal Link: Investigating Foodborne Disease Outbreaks
William D. Marler, Esq.
Hyla Middle School Science
CDC Estimates of Foodborne Illness
• 48 million cases of foodborne illness annually
• 125,000 hospitalizations
• 3,000 deaths
Estimates Differ From Actual Counts
• Annual E. coli O157 estimates
– 62,000 illnesses
– 1,800 hospitalizations
– 52 deaths
• But, only 2,621 E. coli 0157 cases were reported in 2005
Notifiable/Reportable Diseases
• Reporting authorized by Congress in 1878
• Nationally Reportable Diseases- 1993 (food or water borne origin)
Botulism, Cryptosporidiosis, Cyclosporiasis, Giardiasis, Hemolytic Uremic Syndrome (post-diarrheal), Hepatitis A, Listeriosis, Salmonellosis, Shiga toxin producing Escherichia coli (STEC), Shigellosis, Trichinosis, Vibriosis
http://www.cdc.gov/ncphi/disss/nndss/phs/infdis2008.htm
Ill person
Specimen collection
Pathway of a Foodborne Illness Investigation
Health Care Provider
Organism identified
Ill person
Organism identified
Specimen collection
Pathway of a Foodborne Illness Investigation
Health Care Provider
Epidemiologic investigation
Public Health Laboratory
If there are more ill persons than expected, an OUTBREAK might be
underway.
How Do We Know If There Is an Excess?
Public Health Surveillance
The ongoing collection, analysis, interpretation and dissemination of health data directed towards the control and prevention of diseases.
Ill person
Organism identified
Specimen collection
Pathway of a Foodborne Illness Investigation
Health Care Provider
Epidemiologic investigation
Public Health Laboratory
Environmental investigation
Product Trace BackPRODUCT RECALL
Typical Steps of an Outbreak Investigation
• Verify the diagnosis• Establish that an outbreak is occurring• Define and identify cases• Orient the data in terms of person, place,
and time• Develop and test the hypotheses• Case Control Study• Refine the hypotheses and carry out additional
studies• Implement control and prevention measures• Report findings
A Word to the Wise!
• No mandatory list of how to proceed
• No set order of steps to take
• Investigation is dynamic: case definition, line listings, descriptive epidemiology, hypotheses can change
• Expect the unexpected
Investigative Partners
• Laboratory investigators– Microbiologic diagnosis
– Virology/Parasitic Labs
– Molecular analysis
• Epidemiologic investigators– Individual case interviews
– Outbreak investigation
• Cohort studies
• Case/control studies
• Environmental investigators– Facility investigation
– Environmental sampling
– Product traceback
Epidemiology–Basic Tools of the Trade
• Symptoms• Incubation• Duration• Food History• Medical Attention• Suspected source• Others Ill
Real-time interviewing with a broad-based exposure questionnaire
Pulsed Field Gel Electrophoresis (PFGE)
• Process separates chromosomal fragments of intact bacterial genomic DNA grown from patient isolate
• Results in 10 to 20 DNA fragments which distinguish bacterial strains
• Genetic relatedness among strains is based on similarities of the DNA patterns
• Outbreak strains are those that are epidemiologically linked AND genetically linked
A Powerful Outbreak Detection Tool
Questions to Consider in Assessing PFGE Clusters
• How common is thePFGE subtype?
• How many cases are there?• Over what time frame
did cases occur?• What is the geographic
distribution of cases?• What are the case
demographics? • Do any of the cases
have a “red flag” exposure?
E. coli O157:H7 Outbreak, Minnesota, September 2005
Team Diarrhea
Thanks to MN DOH “Team D” for use of the following slides!
Outbreak Detection
September 27, 2005
• Three O157 isolates with indistinguishable PFGE patterns identified by Minnesota Public Health Laboratory
• PFGE pattern new in Minnesota, rare in United States
– 0.35% of patterns in National Database
• Patients reported eating prepackaged salad; no other potential common exposures evident
E. coli O157:H7 Cases Associated with Dole Prepackaged Lettuce
Date of Onset 2005
15 16 17 18 19 20 21 22 23 24 25
Num
ber
of
Case
s
26 27 28 29 30 1 2 3 414
1
2
3
4
5
6
7
September October
Initial cluster of 3 isolates among MN residents identified.
Outbreak Investigation - Methods
September 28–29, 2005
• Additional O157 isolates received at the MDOH and subtyped by PFGE
– 7 isolates demonstrated outbreak PFGE subtype
• Supplemental interview form created
• Case-control study initiated
E. coli O157:H7 Cases Associated with Dole Prepackaged Lettuce
Date of Onset 2005
15 16 17 18 19 20 21 22 23 24 25
Num
ber
of
Case
s
26 27 28 29 30 1 2 3 414
1
2
3
4
5
6
7
September October
Initial cluster of 3 isolates among MN residents identified.
Case-control study initiated.
Case-Control Study Results
Exposure Cases Controls p-valueMatched OR*95% CI†
*OR = odds ratio† CI = confidence interval
Any lettuce9/10 17/26 3.5 0.5–25.0
9/10Prepackaged lettuce salad 10/26 8.4 1.2–59.6
Dole prepackaged lettuce salad9/10 5/23
0.17
0.01
0.00210.1 1.5–67.3
E. coli O157:H7 Cases Associated with Dole Prepackaged Lettuce
Date of Onset 2005
15 16 17 18 19 20 21 22 23 24 25
Num
ber
of
Case
s
26 27 28 29 30 1 2 3 414
1
2
3
4
5
6
7
September October
Initial cluster of 3 isolates among MN residents identified.
Case-control study initiated.
Case-control study implicated Dole salad.
E. coli O157:H7 Cases Associated with Dole Prepackaged Lettuce
Date of Onset 2005
15 16 17 18 19 20 21 22 23 24 25
Num
ber
of
Case
s
26 27 28 29 30 1 2 3 414
1
2
3
4
5
6
7
September October
Initial cluster of 3 isolates among MN residents identified.
Case-control study initiated.
Case-control study implicated Dole salad.
CDC, FDA notified.
E. coli O157:H7 Cases Associated with Dole Prepackaged Lettuce
Date of Onset 2005
15 16 17 18 19 20 21 22 23 24 25
Num
ber
of
Case
s
26 27 28 29 30 1 2 3 414
1
2
3
4
5
6
7
September October
Initial cluster of 3 isolates among MN residents identified.
Case-control study initiated.
Case-control study implicated Dole salad.
CDC, FDA notified.
Minnesota
Additional states
Date of Onset 2005
15 16 17 18 19 20 21 22 23 24 25
Num
ber
of
Case
s
26 27 28 29 30 1 2 3 414
1
2
3
4
5
6
7
September October
WI
WI
OR
E. coli O157:H7 Cases Associated with Dole Prepackaged Lettuce (N=26)
Shared common "Best if Used By” Date and production code
Dole Classic Romaine Salad Recovered from Case-Households
Product Traceback
• Single processing plant (Soledad, CA)• Production Date of September 7, 2005• Lettuce harvested from any 1 of 7 fields
PFGE Patterns of E. coli O157:H7 Isolates from Lettuce
SourceInitial Minnesota Case-patient
Classic RomaineBag #2
Classic RomaineBag #1
Why Epidemiologic Links May Not be Identified for Cases in a PFGE Cluster
• Cases have imperfect recall
• Common exposures can be difficult to link (e.g., eggs, chicken)
• Secondary transmission
• Cross-contamination exposure
• There isn’t a common source
CDC 2005 Cluster Investigations
E. coli O157 SalmonellaPatterns Submitted 5,37629,168Clusters Identified 67 176Multi-state Clusters 36 152Epi Investigation 19 30Vehicle Implicated 4 8Regulatory Activity 4 8
Questions?