قسم المكونات Department of components

Department of components In The Central Blood Bank

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6Whole blood

Red cells

Plasma

Platelets

(Fresh) frozen plasma (FFP)

Cryoprecipitate

Stored Plasma

F lX

Immuno- globulins

AlbuminFractionated products

F VllaF [email protected]

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1- Red blood cell concentratesWhole BloodRed cell concentrateTotal Volume500 ml300 mlVolume of red cells200 ml200 mlVolume of plasma300 ml100 mlHematocrit40 %70 %

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:- 1- 450 + 10 %2- :- CPD 63 AS-5, SAGM,ADSOL( 100 ) .3- :- 10 , .4- :- (20-24 ) 6-8 .

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( ) ( ). . 405 ml - 495 ml 429 gm 525 gm (90 gm) 519 gm 615 gm 300 ml 350 ml ( ) , ( ) ( , ).

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4 8 (20-24 ) . . . . . .

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CPD-SAGM (centrifuge ) plasma extractor 1,2 (clamp) SAGM SAGM . [email protected]

CPD-SAGM . [email protected]

Platelets concentration preparation [email protected]

Platelets concentration preparation : [email protected]

50 60 plat. Agitator +20 +24 3-5 quarantine -30 [email protected]

21 Plasma Derivatives

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Thaw at 4oC & heavy spinFresh Frozen PlasmaCryoprecipitate-Refrozen within 1 hr-Store at < - 18oC Cryoremoved PlasmaFreeze -80oC immediatelyStored at < [email protected]

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Plasma Derivatives

Plasma DerivativesPreparation avaliableCoagulation FactorsFactor VIII concentratesFactor IX concentratesAnti-thrombin IIIAlbuminAlbuminPlasma protein fractionImmune globulinsNon-specific immune serum globulin (ISG)Rh immune globulin (RhIG)Hepatitis B immune globulin (HBIG)Varicella-Zoster immune globulin (VZIG)Tetanus immune globulin (TIG)

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Cryoprecipitate

+ - 4 10- 20 , 100-80 150 300 . :- 6 [email protected]

Cryoprecipitate 2 - 8 8 -24 . . extractor plasma . 20 - 30 . - 30 [email protected]

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+ 35 10 . 6 loop plasma transfer set . 6 loop transfer set . - 30 [email protected]

Differences of Cryoprecipitate & Factor VIII concentrates CryoprecipitateFactor VIII concentratesStorage Temp.-30oC4oCShort period RTRisk of HepatitisLowHighTreatment of hemophilia AYesYesTreatment of vW disease Yesno

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28Blood products & treatment of specific clotting factor deficienciesDeficiencyBlood product IndicatedFibrinogenCryoprecipitateStored plasmaFactor VFresh frozen plasmaFrozen plasmaFactor VIIFactor IX concentrateStored plasmaFactor VIIIFactor VIII concentrateCryoprecipitateVon Willebrands DiseaseCryoprecipitateFresh frozen plasmaFrozen plasmaFactor IXFactor IX concentrateFactor XStored plasmaFactor XIStored plasmaFactor XIIIStored plasma

washed [email protected]

washed RBCs : luminal flow :- N.S- - 200- 300 [email protected]

washed RBCs - Plasma extract Buffy coat 100 N.S 24 ------------ 24 [email protected]

() Filtration\leukocyteReduced [email protected]

() Filtration\leukocyte Reduced RBCs

:- ((filter ( - -----) (luminal flow) filtration 33

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( x-matching ...,....... 24 [email protected]

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Hettich1- - rpm 3500 - 10 minutes - 22 C2- - rpm 2500 - 5 minutes - 22 C 3- - 4000 rpm - minutes 7 - 6 C

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:- .- . . - . [email protected]

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Weighing balance

Refrigerated Centrifuge 3 3 Platelet incubator Component thawing devices

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12.5 / 450 10% 4 / 4/ 170 70-80 % 0.8% (PH) 4 / 4/ 4 / 65.5 1010 75 % 45-60 . ( PH) 4 / 4 / 6 3 1110 75% ( ) 8 4/ 4 / 80 140 / 8 4/ 235 .170 .210 .50 . 4/ 4/ 90 % 75% 1610

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1- 15 20052-The Blood Cold Chain - WHOGuide to the selection and procurement of equipment and accessories 3- , 2014

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CategoryStorageTransportExpiration

Whole Blood 2-6, unless for room temperature components, then 2-6 C within 8 hours2-10, except after collection if to be used for room temperature components, then 20-24 C

CPD/CP2D: 21 days

CPDA-1: 35 DAYS

Red Blood cells (RBCs)2-6 C2-10 CCPD/CP2D: 21 days

CPDA 1: 35 DAYS

Open system 24 hours

RBCs Additive Solutions2-6 C2-10 C42 days

CategoryStorageTransportExpiration

Platelets Irradiated20-24 C with continuous gentle agitation

2 0-24 COpen system 4 hours

Closed system 5 days

Platelets Leukocytes Reduced20-24 C with continuous gentle agitation

2 0-24 COpen system 4 hours

Closed system 5 days

Platelets Pheresis20-24 C with continuous gentle agitation

2 0-24 C5 days

Platelets Pheresis Irradiated20-24 C with continuous gentle agitation

2 0-24 COpen system:4 hours

Closed system :5 days

Platelets Pheresis Leukocytes reduced20-24 C with continuous gentle agitation20 -24 C5 days

Category Storage Transport Expiration

Granulocytes20-24 C20-24 C4 hours

Cryoprecipitated

AHF< -18 CMaintain frozen state

12 months

Cryoprecipitated

AHF Thawed20-24 C20-24 COpen system: 4 hours

Single unit or pooled

prior to freeing : 6 hours

Fresh Frozen plasma (FFP)< -18 C or

< -65 CMaintain frozen state-18 C :12 months

or -65 C :7 years

FFP Thawed1-6 C1-10 C24 hours

Plasma cryoprecipitate reduced< -18 CMaintain frozen state

12 months

Health & Medicine

قسم المكونات Department of components