PH, COD AND TSS

ASSIGNMENT• Date Tested : December 2,2016• Tested By : 14BCL 153D :14BCL120 :14BCL151D• Project Name : pH, COD and TSS, Environmental Engineering II lab• Sample Location: PDPU campus• Sample Description : lawn(garden)water

pH Analysis

pH AnalysisAIM:

• To determine the pH of garden water collected from PDPU CAMPUS.

APPARATUS:

1. pH meter 2. Standard flasks 3. Magnetic Stirrer 4. Funnel 5. Beaker Wash Bottle 6. Tissue Paper 7. Forceps

REAGENTS

1. Buffers Solutions of pH 4.01, 7.0 and 9.2

2. Potassium Chloride

3. Distilled Water

pH AnalysisPRINCIPLE

The pH electrode used in the pH measurement is a combined glass electrode. It consists of sensing half cell and reference half cell, together form an electrode system. The sensing half cell is a thin pH sensitive semi permeable membrane, separating two solutions, viz., the outer solution, the sample to be analysed and the internal solution, enclosed inside the glass membrane and has a known pH value. An electrical potential is developed inside and another electrical potential is developed outside, the difference in the potential is measured and is given as the pH of the sample.

pH AnalysisTheory:

• The term pH refers to the measure of hydrogen ion concentration in a solution and defined as the negative log of H+ ions concentration in water and wastewater. The values of pH 0 to a little less than 7 are termed as acidic and the values of pH a little above 7 to 14 are termed as basic. When the concentration of H+ and OH– ions are equal then it is termed as neutral pH.

pH AnalysisProcedure:

• Collect water from and transfer it into a beaker.• Switch on the pH meter.• Remove electrodes from storage solution and rinse with water.• Bloat with soaked tissue paper.• Standardize the instrument with electrodes immersed in a buffer

solution (Acetate buffer pH = 4).• Rinse, bloat and dry the electrodes to each tie.

pH AnalysisProcedure:

• Check the pH on the pH meter (pH = 7).• Rinse, bloat and dry the electrodes.• Standardize the instrument with electrodes immersed in a buffer solution

(Ammonium buffer pH = 10).• Rinse, bloat and dry the electrodes and check the pH on the pH meter (pH = 7).• Dip the pH electrode in the beaker containing waste water to be tested. Note

the pH.• Replace the electrodes in the storage solution.

pH AnalysisRESULTS• pH: 5.97

TOTAL DISSOLVED SOLIDSAIM

• To determine total dissolved solids in the garden water sample

APPARATUS

1. Evaporating Dish 2. Water Bath 3. Oven 4. Desiccators 5. Analytical Balance

TOTAL DISSOLVED SOLIDSPRINCIPLE

• A well mixed sample is filtered through a standard glass fiber filter, and the filtrate is evaporated to dryness in a weighed dish and dried to constant weight at 179- 181°C. The increase in dish weight represents the total dissolved solids. A well mixed sample is filtered through a weighed standard glass fiber filter and the residue retained on the filter is dried to a constant weight at 103-105°C. The increase n weight of the filter represents the total suspended solids. If the suspended material clogs the filter and prolongs filtration, the difference between the total solids and total dissolved solids may provide an estimate of the total suspended solids

TOTAL DISSOLVED SOLIDSTHEORY

The term total dissolved solids refer to materials that are completely dissolved in water. These solids are filterable in nature. It is defined as residue upon evaporation of filterable sample. The term total suspended solids can be referred to materials which are not dissolved in water and are non filterable in nature. It is defined as residue upon evaporation of non filterable sample on a filter paper.

TOTAL DISSOLVED SOLIDSPROCEDURE

• To measure total dissolved solids, take a clean porcelain dish which has been washed and dried in a hot air oven at 180(C for one hour.

• Now weigh the empty evaporating dish in analytical balance. • Let’s denote the weight measured as W1 = 38.47 g. • Mix sample well and pour into a funnel with filter paper. Filter approximately 80 -

100 mL of sample. • Using pipette transfer 75mL of unfiltered sample in the porcelain dish.


• Switch on the oven and allowed to reach 105°C. Check and regulate oven and furnace temperatures frequently to maintain the desired temperature range.

• Place it in the hot air oven and care should be taken to prevent splattering of sample during evaporation or boiling.

• Dry the sample to get constant mass. Drying for long duration usually 1 to 2 hours is done to eliminate necessity of checking for constant mass.


• Cool the container in a desiccator. Desiccators are designed to provide an environment of standard dryness. This is maintained by the desiccant found inside. Don't leave the lid off for prolonged periods or the desiccant will soon be exhausted. Keep desiccator cover greased with the appropriate type of lubricant in order to seal the desiccator and prevent moisture from entering the desiccator as the test glassware cools.

• We should weigh the dish as soon as it has cooled to avoid absorption of moisture due to its hygroscopic nature. Samples need to be measured accurately, weighed carefully, and dried and cooled completely.

• Note the weight with residue as W2 = …………………….…g

TOTAL DISSOLVED SOLIDS• Calculation

mg total suspended solids/L=--------------------

(A-B) X 1000

sample volume, mL

TOTAL DISSOLVED SOLIDSRESULTS

• TSS in garden water =

CHEMICAL OXYGEN DEMAND AIM

• To determine chemical oxygen demand in the garden water sample

CHEMICAL OXYGEN DEMAND APPARATUS

1. COD Digester 2. Burette & Burette stand 3. COD Vials with stand 4. 250 mL conical flask 5. Pipettes 6. Pipette bulb 7. Tissue papers 8. Wash Bottle

REAGENTS

1. Potassium dichromate 2. Sulfuric acid 3. Ferrous ammonium sulphate 4. Silver sulphate 5. Mercury sulphate 6. Ferroin indicator 7. Organic free distilled water

CHEMICAL OXYGEN DEMAND PRINCIPLE

The organic matter present in sample gets oxidized completely by potassium dichromate (K2Cr2O7) in the presence of sulphuric acid (H2SO4), silver sulphate (AgSO4) and mercury sulphate (HgSO4) to produce CO2 and H2O. The sample is refluxed with a known amount of potassium dichromate (K2Cr2O7) in the sulphuric acid medium and the excess potassium dichromate (K2Cr2O7) is determined by titration against ferrous ammonium sulphate, using ferroin as an indicator. The dichromate consumed by the sample is equivalent to the amount of O2 required to oxidize the organic matter.

CHEMICAL OXYGEN DEMAND THEORY

• Before performing this experiment, few questions may arise to the learners:1. What is meant by chemical oxygen demand? 2. Why do we need to determine COD? 3. What are the methods available to measure COD?4. Is it measured in water or wastewater? 5. Whether is it mandatory to determine COD as per our codal provision? • The chemical oxygen demand (COD) test is commonly used to indirectly measure the amount

of organic compounds in water. Most applications of COD determine the amount of organic pollutants found in surface water (e.g. lakes and rivers), making COD a useful measure of water quality.

CHEMICAL OXYGEN DEMAND THEORY

• It is expressed in milligrams per litre (mg/L), which indicates the mass of oxygen consumed per litre of solution.

• COD is the measurement of the amount of oxygen in water consumed for chemical oxidation of pollutants.

• COD determines the quantity of oxygen required to oxidize the organic matter in water or waste water sample, under specific conditions of oxidizing agent, temperature, and time.

• This method covers the determination of COD• in ground and surface waters, domestic and industrial wastewaters. The applicable range

is 3-900 mg/L.

CHEMICAL OXYGEN DEMAND PROCEDURE

1. Wash culture tubes and caps with 20% H2SO4 before using to prevent contamination. 2. Place sample (2.5 mL) in culture tube and Add K2Cr2O7 digestion solution (1.5 mL). 3. Carefully run sulphuric acid reagent (2.5 mL) down inside of vessel so an acid layer is formed under the sample-digestion solution layer and tightly cap tubes or seal ampules, and invert each several times to mix completely. 4. Place tubes in block digester preheated to 150°C and reflux for 2 h behind a protective shield.

CHEMICAL OXYGEN DEMAND PROCEDURE

5. Cool to room temperature and place vessels in test tube rack. Some mercuric sulphate may precipitate out but this will not affect the analysis. 6. Add 1 to 2 drops of Ferroin indicator and stir rapidly on magnetic stirrer while titrating with standardized 0.10 M FAS. 7.The end point is a sharp colour change from blue-green to reddish brown, although the blue green may reappear within minutes. 8. In the same manner reflux and titrate a blank containing the reagents and a volume of distilled water equal to that of the sample.

CHEMICAL OXYGEN DEMAND CALCULATION

• COD is given by COD (mg O2 /L) = [(A-B) × M ×8000) / (V sample) Where: A = volume of FAS used for blank (mL) B = volume of FAS used for sample (mL) M = molarity of FAS 8000 = mL equivalent weight of oxygen (8) ×1000 mL/L.

CHEMICAL OXYGEN DEMAND CALCULATION

CHEMICAL OXYGEN DEMAND RESULTS

COD OF SAMPLE: 67.2 mg/l

CHEMICAL OXYGEN DEMAND CONCLUSION