Weiss labInternship fall 2014

Snowing

Mentor

Franzi Uhl

• PhD in natural science

• Masters in bioengineering

• From Germany

• Thanks for being an awesome

mentor

What I did

Projects Human and Pig lung decel

photo protocol

Science fair: preventing contamination in decellularizedmouse lungs

Responsibilities decelling mouse lungs

Removing mouse lungs from mice

Helping with human and pig lung decell

Mixing solutions

Desk work

Mixing solutions

Measuring liquids, using scale, autoclaving, sterile filtering etc.

Preventing

contamination in

decellurized mouse

lungsScience fair project 2014 - 15

• WHY

• Many people need lung transplants

• decellularizing is the first step to

growing lungs

• Experiments are done on mouse lungs

• decellularized mouse lungs often get

contaminated with bacteria

Science fair project

• WHAT:

• Researching pen-strep

antibiotics, agar, etc

• growing bacteria from samples

taken each day of decell, to find

out when contamination occurs

• Doubling the quantity of

penicillin-streptomycin to

prevent contamination

• RESULTS:

• Coming next term

Pig lung decellularization photo protocol

How to make agar plates Teaching a skill

Mix agar and DI water

Mix 23 grams agar per

Liter of water

Microwave In 1 minute

intervals until agar has

dissolved(about 2 minutes)

Mixing agar

Autoclave agar to insure

sterility

Allow to cool

Label all solutions with

content initials and date

Teaching a skill

Pouring the plates

Make sure agar is a

liquid. If not

microwave to

liquify

Tip up lid of plate

and pore agar slowly

to avoid bubbles Seal plate with

parafilm, store upside-

down in fridge

What I learned

Decellularizing pig and human lungs

Using google drawing program

Making agar

Writing protocol for science fair