Weiss labInternship fall 2014

Snowing

Mentor

Franzi Uhl

• PhD in natural science• Masters in bioengineering • From Germany• Thanks for being an

awesome mentor

What I did

Projects Human and Pig lung decel

photo protocol

Science fair: preventing contamination in decellularized mouse lungs

Responsibilities decelling mouse lungs Removing mouse

lungs from mice Helping with human

and pig lung decell Mixing solutions Desk work

Mixing solutionsMeasuring liquids, using scale, autoclaving, sterile filtering etc.

Preventing contamination in decellurized mouse lungsScience fair project 2014 - 15

• WHY

• Many people need lung transplants

• decellularizing is the first step to growing lungs

• Experiments are done on mouse lungs

• decellularized mouse lungs often get contaminated with bacteria

Science fair project

• WHAT:

• Researching pen-strep antibiotics, agar, etc

• growing bacteria from samples taken each day of decell, to find out when contamination occurs

• Doubling the quantity of penicillin-streptomycin to prevent contamination

• RESULTS:

• Coming next term

Pig lung decellularization photo protocol

How to make agar plates Teaching a skill

Mix agar and DI waterMix 23 grams agar per Liter of water

Microwave In 1 minute intervals until agar has dissolved(about 2 minutes)

Mixing agar

Autoclave agar to insure sterility

Allow to coolLabel all solutions with content initials and date

Teaching a skill

Pouring the plates

Make sure agar is a liquid. If not microwave to liquify

Tip up lid of plate and pore agar slowly to avoid bubbles

Seal plate with parafilm, store upside-down in fridge

What I learned

Decellularizing pig and human lungs

Using google drawing program

Making agar

Writing protocol for science fair