Welcome

MASS REARING TECHNIQUES OF HELICOVERPA ARMIGERA

Guided toDr.M.K.Nayak

Presented byKavita bisenRoll No.-5765

DEPARTMENT OF ENTOMOLOGY

KRISHI MAHA VIDYALAYA, TIKAMGARH, (M. P.)

MASS REARING OF HELICOVERPA armigera

COMMON NAME - GRAM POD BORER

S.N. - Helicoverpa armigera

ORDER - Lepidoptera

FAMILY – Noctuidae

BIOLOGY -

Biology of the gram pod borer reared on Bengal

gram and on artificial diet is given in table

Life stage Bengal gram natural food

Artificial diet

Larval period (day) 25 22.10Pupal period (day) 11 14.10Male (day) 6 7.8Female (day) 10 9Fecundity (egg/family)

300 130

Incubation period(day)

4.5 4.9

Viability of egg(%) 90 86

Equipment and facilities Food material (viz.-macerated paste of soaked bengal gram

seeds), distilled water, glass tube(10×2.5 cm.), jars(20×15 cm.),

cotton wool, markin cloth, smooth hair brush, autoclave and a

room fitted with all amentias for rearing the insect as explained

here.

REARING freshly hatched larvae of pod borer may be fed on

macerated pest of soaked bengal gram seeds for 2-3 days later,

they are separated and reared in individually tubes (10×2.5 cm.)

till population takes place by placing 2-8 grains of soaked bengal

gram seeds.

everyday normally , population takes place inside the

cotton plug used for securing the host inside the

tube. pupae are sexed and kept separately. The

techniques as also used for other Lepidopteron

pupae the emerged adults are kept in our of (20×15

cm.) for oviposition and a cotton scab clipped in

sugar solution (10%) is hung in the jars . larvae can be

reared on artificial diet by placing 8 gm. Cake for each

individual larvae into a tube to avoid cannibolism.

it should be reared at 25±2 C and 60±5% RH.⁰

Precaution

1. Rear second instar larvae to individual

tubes to avoid cannibolism

2. Do not case more than 5 pairs per jar for

good oviposition.

3. Starts next generation from the first batch

oviposition reared from healthy adults.

4. Always sterilized the diet and cotton plugs

in an autoclave.

5. First treat eggs with sodium by chloride

(6.157%) and then with Na thiosulphate

(10%) to separate them from the

substratum. Which also acts as surface

strilant.

Thank-you