Genome sequencing for animal health – input and impact

Gwenaelle DAUPHINAGAH – EMPRES

Lab Unit coordinator

https://www.google.co.uk/url?url=https://en.wikipedia.org/wiki/National_Center_for_Biotechnology_Information&rct=j&frm=1&q=&esrc=s&sa=U&ved=0ahUKEwim7tn6vqbPAhWCF8AKHQEeBQMQwW4IFjAA&usg=AFQjCNHhK4bDQPcUTKpTQWIJ9T7zcJa6_A

Running PCR

Obtaining genetic sequence

Analysing and sharing

Monitoring virus distribution

Monitoring virus genetic evolution

Monitoring vaccine matching

More powerful molecular epidemiology

Better design of diagnostic assays

Improved veterinary vaccination

Molecular DIVA for CapripoxvirusesCapripoxvirus Genotyping

Whole genome phylogenetic analysis of Capripoxviruses

Whole genome sequencing

Outcomes and impact in Indonesia: Influenza Virus Monitoring Network

Informed Avian influenza control policies, including vaccination

Improved knowledge of circulating H5N1 viruses

Clear process to support decision making

Endemic with influenza H5N1 since 2004: -3,500 poultry H5N1 outbreaks reported-1,150 Egyptian influenza H5N1isolates from poultry which sequences = deposited to the public domain

Outcomes and impact in Egypt

Phylogenetic analysis of A(H5N1) in Egypt during 2006-2014

Conclusions• Sequence data: key component in the control and prevention of animal and zoonotic

diseases• Molecular biology used in all countries - use of sequencing is expanding – high

demand for training• Sharing of sequences of utmost importance

active in building capacities, assisting countries to apply sequencing and promoting the use and sharing of sequences