Arsenic Biosensor

Presented by :Md.Waliullah WaliID# 15276002Department of M.N.S.BRAC University

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Cell-free arsenic biosensors

DNA- based biosensors

Protein-based Biosensors

Aptamers-based biosensors


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Signal

Working Principle of DNA- based biosensors


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Arsenic

Signal

Oxidative damage

Limitations

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Poor detection limit Non-specificity Inefficient electrode regeneration Sophisticated electrode preparation It lacked specificity towards As(III)

Aptamers - based biosensors

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Aptamers are single-stranded DNA or RNA molecules that can bind to a number of target analytes, including proteins and peptides with high affinity and specificity.

Commercial potential for use as biosensors.


7Structure of Aptamer


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Possible mode of interaction of arsenic site with aptamer


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Aptamers

Arsenic

Cationic Polymers/ Surfactants

Signal


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Aptamer Ars-3 Polydiallyldimethyl-ammonium

(PDDA)

Arsenic

Protein-based biosensors

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Most protein-based biosensors developed for As(III) or As(V) are based on the inhibition phenomenon.

Interaction between protein and arsenic species

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Characteristics of cell free arsenic biosensors with detection limits and induction period/response time

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Conclusion

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A number of arsenic biosensors have been developed based on whole-cell biosensor to biomolecules based biosensors. However, whole-cell biosensor has successfully utilized in the analysis of arsenic in groundwater and soil, but has some limitations.Biomolecules based biosensors has quick response capacity and better detection limits.

Further challenges

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Development of biosensors that could detect arsenic in complex matrices including health related matrices such as blood, urine, etc. and water with high TDS and salinity including seawater.

Article info

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Thank you All

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Education

Arsenic Biosensor