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Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
Presented by:Fiddy S. PRASETIYA
Supervised by:Jean-Luc MOUGETBruno COGNIEMichèle MORANCAISYann HARDIVILLIERRomain GASTINEAU
Fiddy SEMBA PRASETIYA, 1st year PhD student Université du MaineStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
• To determine the allelopathic effect of H. ostrearia and external marennine on the growth of other species of diatoms
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
2
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
3
Culture preparation:- H. ostrearia- T. suecica- C. gracilis- I. galbana (T. Iso)- S. costatum- P. lutheri- C. calcitrans
Measurement of :- Biovolume - Growth rate
Preparation of allelopathy experiment:- H. ostrearia + T. suecica, etc
(based on biovolume comparison), vol. max. 150 ml
Observation algal growth and biovolume under microscope,
Marennine in each culture sampled (10 days)for HPLC analysis
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
4
Comparison of biovolume & growth rate of each diatoms species
0 4 6 8 10 12 140
100
200
300
400
500
600
700
Growth rate of control
H.ostreariaT.suecica C.gracilisI.galbanaS.costatum C.calcitransP.lutheri
Time (days)Cell
conc
entr
ation
[x10
⁴] (
cell/
mL)
0 4 6 8 10 12 140
10
20
30
40
50
60
70
80
Biovolume of control
H.ostreariaT.suecica C.gracilisI.galbanaS.costatum C.calcitransP.lutheri
Time (days)
Volu
me
(µL)
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
5
0 4 6 8 100
20
40
60
80
100
120
140
Conc
entr
ation
(x10
4 ce
ll/m
l)
0 4 6 8 100.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
T. suecica (Control)T. suecica (treatement)H.ostrearia (Control)H.ostrearia (+T.suecica)Vo
lum
e (µ
L)
Comparison of allelopathic effect on growth rate & biovolume of T. Suecica & C. gracilis
0 4 6 8 100
20
40
60
80
100
120
Times (days)
Conc
entr
ation
(x 1
0⁴ C
ell/
mL)
0 4 6 8 100.000
10.000
20.000
30.000
40.000
50.000
60.000
C.gracilis (Control)C.gracilis (treatement)H.ostrearia (Control)H.ostrearia (+C.gracilis)
Time (days)
Volu
me
(µL)
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
6
Comparison of allelopathic effect on growth rate & biovolume of T. iso (16°) & T. iso (21°)
0 4 6 8 100
100
200
300
400
500
600
Conc
entr
ation
(x10
4 ce
ll/m
l)
0 4 6 8 100.000
10.000
20.000
30.000
40.000
50.000
T. iso (Control)T. iso (treatement)H.ostrearia (Control)H.ostrearia (+T. iso)Vo
lum
e (µ
L)
0 4 6 8 100
100
200
300
400
500
600
Time (days)
Conc
entr
ation
(x10
4 ce
ll/m
l)
0 4 6 8 100
10
20
30
40
50
T. iso 21 (Control)T. iso 21 (treatement)H.ostrearia (Control)H.ostrearia (+ T. iso 21 )
Time (days)
Volu
me
(µL)
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
7
Comparison of allelopathic effect on growth rate & biovolume of S. costatum & C. Calcitrans
0 4 6 8 100
20
40
60
80
100
120
Conc
entr
ation
(x10
4 ce
ll/m
l)
0 4 6 8 100.00
10.00
20.00
30.00
40.00
S. costatum (Control)
S. costatum (treatement)
H.ostrearia (Control)
H.ostrearia (+S. costatum)
Volu
me
(µL)
0 4 6 8 100
20
40
60
80
100
120
Times (days)
Conc
entr
ation
(x 1
0⁴ C
ell/
mL)
1 2 3 4 50.000
10.000
20.000
30.000
40.000
50.000
60.000
C.calcitrans (Control)
C.cancitrans (treatement)
H.ostrearia (Control)
H.ostrearia (+C.calcitrans)
Time (days)
Volu
me
(µL)
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYAStudy of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
8
Comparison of allelopathic effect on growth rate & biovolume of P. lutheri
0 4 6 8 100
20
40
60
80
100
120
Time (days)
Conc
entr
ation
(x10
4 ce
ll/m
l)
0 4 6 8 100.00
10.00
20.00
30.00
40.00
50.00
P. Lutheri (Control)
P. Lutheri (treatement)
H.ostrearia (Control)
H.ostrearia (+P. Lutheri)
Time (days)
Volu
me
(µL)
