WP8: Analysis of antioxidants (tocols)
CRA-MACNicola Berardo
Sabrina LocatelliLorenzo Guerrini
Rita Redaelli
AVEQ final Meeting – Bucarest, 18-22 October 2010
AVEQ final Meeting – Bucarest, 18-22 October 2010
Method for analysis
Reducing Time Analysis for the Determination of Tocols in Cereals by Normal-Phase High Performance Liquid Chromatography
V. Pisacane, R. Redaelli and N. Berardo
J. Genet. & Breed. 58: 253-258 (2004)
AVEQ final Meeting – Bucarest, 18-22 October 2010
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STANDARD MIX OF TOCOLS - CHROMATOGRAM
AVEQ final Meeting – Bucarest, 18-22 October 2010
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TOCOLS IN A SAMPLE - CHROMATOGRAM
AVEQ final Meeting – Bucarest, 18-22 October 2010
Tocopherols α-T β-T γ-T δ-T TT
Mean 12.27 2.14 0.19 0.12 14.73
Min 8.06 1.16 0.00 0.00 9.40
Max 18.60 3.93 1.14 1.01 21.09
SD 2.53 0.68 0.25 0.22 2.76
BULGARIA - STANDARD CULTIVARS
Tocotrienols α-T3 β-T3 γ-T3δ-T3
TT3 Tocols
Mean 24.64 5.34 1.60 1.26 32.67 46.95
Min 14.57 3.46 0.00 0.00 18.89 20.94
Max 49.83 11.79 3.67 2.33 65.74 85.43
SD 7.43 1.78 0.82 0.59 8.60 10.90
AVEQ final Meeting – Bucarest, 18-22 October 2010
α-T β-T γ-T δ-T TT
Mean 23.83 1.67 1.57 0.36 27.43
Min 7.66 0.00 0.00 0.00 10.17
Max 50.30 5.61 5.76 1.67 51.99
SD 9.97 1.37 2.21 0.43 10.13
BULGARIA – SELECTED ACCESSIONS
α-T3 β-T3 γ-T3 δ-T3 TT3 Tocols
Mean 45.27 3.29 0.27 0.33 49.07 75.48
Min 22.81 0.02 0.00 0.00 27.59 39.68
Max 102.76 12.86 2.30 1.38 107.85 159.84
SD 16.73 2.53 0.39 0.35 17.56 22.24
AVEQ final Meeting – Bucarest, 18-22 October 2010
Bulgaria 2008
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Tocols (mg kg-1 dm)
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AVEQ final Meeting – Bucarest, 18-22 October 2010
Bulgaria 2008
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-tocopherol (mg kg-1 dm)
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AVEQ final Meeting – Bucarest, 18-22 October 2010
Bulgaria 2008
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-tocotrienol (mg kg-1dm)
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AVEQ final Meeting – Bucarest, 18-22 October 2010
Tocopherols
α-T β-T γ-T δ-T TT
Mean 8.17 2.46 0.04 0.04 10.71
Min 5.80 1.10 0.00 0.00 7.42
Max 12.63
5.23 0.30 0.30 15.53
SD 1.43 1.00 0.08 0.06 1.91
ESTONIA - STANDARD CULTIVARS
Tocotrienols
α-T3 β-T3 γ-T3δ-T3
TT3 Tocols
Mean 12.85 5.85 1.06 0.95 20.56 30.92
Min 7.02 3.67 0.00 0.00 9.37 10.83
Max 32.26 8.34 2.94 2.09 41.27 53.88
SD 4.20 1.38 0.54 0.67 5.07 6.94
AVEQ final Meeting – Bucarest, 18-22 October 2010
Tocopherols α-T β-T γ-T δ-T TT
Mean 28.59 2.08 0.06 0.07 30.80
Min 5.64 0.78 0.00 0.00 7.61
Max 65.17 4.49 0.56 0.86 68.60
SD 18.13 0.66 0.13 0.12 18.02
ESTONIA – SELECTED ACCESSIONS
Tocotrienols α-T3 β-T3 γ-T3 δ-T3 TT3 Tocols
Mean 19.97 5.40 0.79 0.29 26.45 57.25
Min 4.42 3.09 0.00 0.00 8.91 26.57
Max 58.12 12.13 2.40 1.60 62.29 116.50
SD 9.34 1.41 0.66 0.32 9.59 20.86
AVEQ final Meeting – Bucarest, 18-22 October 2010
Estonia 2008
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Tocols (mg kg-1 dm)
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Estonia 2008
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-tocopherol (mg kg-1 dm)
Distribution
AVEQ final Meeting – Bucarest, 18-22 October 2010
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tocotrienol ( mg kg-1 dm)
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AVEQ final Meeting – Bucarest, 18-22 October 2010
STANDARD CULTIVARS – ESTONIA E BULGARIA 2008
TAXA Estonia Bulgaria
A. abyssinica 41.76 62.88
A. barbata subsp. barbata 116.50 84.67
A. byzantina 41.66 64.81
A. byzantina subsp. byzantina 56.68 68.16
A. sativa 53.02 72.70
A. sativa subsp. nudisativa 47.19 78.47
A. sativa subsp. sativa var. aristata 46.87 70.64
A. sativa subsp. sativa var. aurea 64.47 68.75
A. sativa subsp. sativa var. brunnea 55.80 59.09
A. sativa subsp. sativa var. cinerea 37.27 77.40
A. sativa subsp. sativa var. grisea 88.08 68.68
A. sativa subsp. sativa var. iranica 33.64 65.05
A. sativa subsp. sativa var. montana 72.10 110.82
A. sativa subsp. sativa var. mutica 56.96 70.01
A. sativa subsp. sativa var. obtusata 46.69 48.68
A. sativa subsp. sativa var. persica 42.57 68.80
A. sativa subsp. sativa var. tristis 54.01 84.44
A. sativa var. brachytricha 67.46 39.68
A. sterilis subsp. sterilis 45.84 90.20
A. strigosa 81.78 110.73
A. strigosa subsp. brevis var. nigricans 43.95 159.84
A. strigosa var. typica 68.15 99.50
Total tocols content
mg kg-1 dm