WP8: Analysis of antioxidants (tocols)

CRA-MACNicola Berardo

Sabrina LocatelliLorenzo Guerrini

Rita Redaelli

AVEQ final Meeting – Bucarest, 18-22 October 2010

AVEQ final Meeting – Bucarest, 18-22 October 2010

Method for analysis

Reducing Time Analysis for the Determination of Tocols in Cereals by Normal-Phase High Performance Liquid Chromatography

V. Pisacane, R. Redaelli and N. Berardo

J. Genet. & Breed. 58: 253-258 (2004)

AVEQ final Meeting – Bucarest, 18-22 October 2010

Minutes

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STANDARD MIX OF TOCOLS - CHROMATOGRAM

AVEQ final Meeting – Bucarest, 18-22 October 2010

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TOCOLS IN A SAMPLE - CHROMATOGRAM

AVEQ final Meeting – Bucarest, 18-22 October 2010

Tocopherols α-T β-T γ-T δ-T TT

Mean 12.27 2.14 0.19 0.12 14.73

Min 8.06 1.16 0.00 0.00 9.40

Max 18.60 3.93 1.14 1.01 21.09

SD 2.53 0.68 0.25 0.22 2.76

BULGARIA - STANDARD CULTIVARS

Tocotrienols α-T3 β-T3 γ-T3δ-T3

TT3 Tocols

Mean 24.64 5.34 1.60 1.26 32.67 46.95

Min 14.57 3.46 0.00 0.00 18.89 20.94

Max 49.83 11.79 3.67 2.33 65.74 85.43

SD 7.43 1.78 0.82 0.59 8.60 10.90

AVEQ final Meeting – Bucarest, 18-22 October 2010

α-T β-T γ-T δ-T TT

Mean 23.83 1.67 1.57 0.36 27.43

Min 7.66 0.00 0.00 0.00 10.17

Max 50.30 5.61 5.76 1.67 51.99

SD 9.97 1.37 2.21 0.43 10.13

BULGARIA – SELECTED ACCESSIONS

α-T3 β-T3 γ-T3 δ-T3 TT3 Tocols

Mean 45.27 3.29 0.27 0.33 49.07 75.48

Min 22.81 0.02 0.00 0.00 27.59 39.68

Max 102.76 12.86 2.30 1.38 107.85 159.84

SD 16.73 2.53 0.39 0.35 17.56 22.24

AVEQ final Meeting – Bucarest, 18-22 October 2010

Bulgaria 2008

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Tocols (mg kg-1 dm)

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AVEQ final Meeting – Bucarest, 18-22 October 2010

Bulgaria 2008

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-tocopherol (mg kg-1 dm)

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AVEQ final Meeting – Bucarest, 18-22 October 2010

Bulgaria 2008

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-tocotrienol (mg kg-1dm)

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AVEQ final Meeting – Bucarest, 18-22 October 2010

Tocopherols

α-T β-T γ-T δ-T TT

Mean 8.17 2.46 0.04 0.04 10.71

Min 5.80 1.10 0.00 0.00 7.42

Max 12.63

5.23 0.30 0.30 15.53

SD 1.43 1.00 0.08 0.06 1.91

ESTONIA - STANDARD CULTIVARS

Tocotrienols

α-T3 β-T3 γ-T3δ-T3

TT3 Tocols

Mean 12.85 5.85 1.06 0.95 20.56 30.92

Min 7.02 3.67 0.00 0.00 9.37 10.83

Max 32.26 8.34 2.94 2.09 41.27 53.88

SD 4.20 1.38 0.54 0.67 5.07 6.94

AVEQ final Meeting – Bucarest, 18-22 October 2010

Tocopherols α-T β-T γ-T δ-T TT

Mean 28.59 2.08 0.06 0.07 30.80

Min 5.64 0.78 0.00 0.00 7.61

Max 65.17 4.49 0.56 0.86 68.60

SD 18.13 0.66 0.13 0.12 18.02

ESTONIA – SELECTED ACCESSIONS

Tocotrienols α-T3 β-T3 γ-T3 δ-T3 TT3 Tocols

Mean 19.97 5.40 0.79 0.29 26.45 57.25

Min 4.42 3.09 0.00 0.00 8.91 26.57

Max 58.12 12.13 2.40 1.60 62.29 116.50

SD 9.34 1.41 0.66 0.32 9.59 20.86

AVEQ final Meeting – Bucarest, 18-22 October 2010

Estonia 2008

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Tocols (mg kg-1 dm)

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AVEQ final Meeting – Bucarest, 18-22 October 2010

Estonia 2008

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-tocopherol (mg kg-1 dm)

Distribution

AVEQ final Meeting – Bucarest, 18-22 October 2010

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tocotrienol ( mg kg-1 dm)

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AVEQ final Meeting – Bucarest, 18-22 October 2010

STANDARD CULTIVARS – ESTONIA E BULGARIA 2008

TAXA Estonia Bulgaria

A. abyssinica 41.76 62.88

A. barbata subsp. barbata 116.50 84.67

A. byzantina 41.66 64.81

A. byzantina subsp. byzantina 56.68 68.16

A. sativa 53.02 72.70

A. sativa subsp. nudisativa 47.19 78.47

A. sativa subsp. sativa var. aristata 46.87 70.64

A. sativa subsp. sativa var. aurea 64.47 68.75

A. sativa subsp. sativa var. brunnea 55.80 59.09

A. sativa subsp. sativa var. cinerea 37.27 77.40

A. sativa subsp. sativa var. grisea 88.08 68.68

A. sativa subsp. sativa var. iranica 33.64 65.05

A. sativa subsp. sativa var. montana 72.10 110.82

A. sativa subsp. sativa var. mutica 56.96 70.01

A. sativa subsp. sativa var. obtusata 46.69 48.68

A. sativa subsp. sativa var. persica 42.57 68.80

A. sativa subsp. sativa var. tristis 54.01 84.44

A. sativa var. brachytricha 67.46 39.68

A. sterilis subsp. sterilis 45.84 90.20

A. strigosa 81.78 110.73

A. strigosa subsp. brevis var. nigricans 43.95 159.84

A. strigosa var. typica 68.15 99.50

Total tocols content

mg kg-1 dm