Welcome to the Media Cybernetics 2003 Dealer Conference
Silver Spring, San Diego,
Munich and Singapore
August/September 2003
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Introducing Image-Pro Plus 5.0!
• Day One– 9:00AM Welcome & Opening Remarks– 10:00AM-Noon Image Pro Plus Five-0– Noon-1:00PM Lunch– 1:00-2:00PM Image Pro Plus Five-0 (Cont.)– 2:00-3:00PM SharpStack Five-0– 3:00-4:00PM 3D Constructor Five-0– 4:00-5:00PM Scope-Pro Five-0
• Day Two– 9:00-11:00AM What is IQbase?– 11:00-Noon How Can WE Demonstrate & Sell IQbase?– Noon-1:00PM Lunch– 1:00-2:00PM Business Meeting– 2:00PM Adjourn
Agenda for 2-Day Program
Image-Pro Plus 5.0More Answers on your Images!
Image-Pro PlusImage-Pro PlusFive-OFive-O
Image-Pro Plus 5.0
Some Highlights
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Introducing Image-Pro Plus 5.0!
• Alignment Correction
• Automatic Tiling and Stitching
• Tracking– Automatic and manual– Measurement Display Options
Exciting New Features
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• Improved Stack Format Support– Handle stacks better and automatically
assign colors where applicable.
• Color Composite Improvements– Customize Dyes and Colors– Image Set and Existing Image Knowledge
• New Basic Capture Dialog
Introducing Image-Pro Plus 5.0!Exciting New Features
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Introducing Image-Pro Plus 5.0!
• New Processing and Display Tools– Despeckle Filter– L*A*B Color– Scale Bar– EDF Test Strips
– Local Zoom– Synchronized Playback of
Sequences– Synchronized Pan and Zoom– Surface Plot Overlay from EDF
Exciting New Features
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Introducing Image-Pro Plus 5.0!
• Snap to New Image– Allows users to automatically burn
measurement overlays onto an image
• Set Manager– Multidimensional viewer - includes aspects of
the improved sequence file support
• Spatial Calibration Improvements– List Management– System Calibration– Wizard
Exciting New Features
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Introducing Image-Pro Plus 5.0!
• Workspace Context Menu Additions– Local Zoom, dye tints, set capture AOI appear in
context menu
• Version Information– Helps tech support know which versions of Image-
Pro Plus and any plug-ins are running
• Default Settings Folder– Location where all settings and environment
information is stored for the product.
Exciting New Features
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Introducing Image-Pro Plus 5.0!
• Objective Information File– Important for those that work with microscopes;
links to Scope-Pro and AFA• Display Range/Contrast Enhancement
Interaction– User interface enhancement to make these
tools less independent• Dye Management
– Allows for custom dyes and for user to define color by emission wavelength
– Pre-Defined Dyes– Add New Dyes– Tint Commands
Exciting New Features
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Introducing Image-Pro Plus 5.0!
• New Graphical User Interface
• Toolbar Changes– Toolbar has added functionality
• New Menu Structure– Menu is more logical for new users.
Exciting New Features
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Introducing Image-Pro Plus 5.0!
• Very New! WorkFlow Manager – User defined function– Toolbar oriented
• Very New! User Identities– Login– Basic, Full, Biological, Industrial defaults
Exciting New Features
Image-Pro Plus 5.0
Key Selling points
Hands On
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IPP 5.0 Alignment Correction
• Align images or a sequence in translation, rotation, and/or scaling.
• Potential applications would be alignment of:– images taken over time with movement– channels of multiple images from a beam
splitter– Serial sections– correcting for offset angle of a Greenough (off-
axis) stereo microscope
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IPP 5.0 Alignment Correction
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IPP 5.0 Alignment Correction
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IPP 5.0 Alignment Correction
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• Synchronized Playback of Sequences
• Synchronized Pan and Zoom
Other Tools
New Tools from the Solutions Zone
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• EDF Test Strips and EDF Surface Plot
EDF Tools
New Tools from the Solutions Zone
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EDF Tools
New Tools from the Solutions Zone
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Automatic Tiling and Stitching
• Align and stitch images together using a Fourier transform edge detection algorithm based upon both pattern and contrast matching
• Options provide for the insertion of blank images, image order modification, and manual adjustment of pane location
• This will be useful for constructing panoramic views or for linking together multiple images taken with a motorized or non-motorized stage.
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Automatic Tiling and Stitching
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Automatic Tiling and Stitching
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Automatic Tiling and Stitching
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Tracking
• Manual Tracking– Click on objects frame by frame
• Automatic Tracking– Highlight objects of interest and let Image-Pro do the
heavy lifting
• Graphs generated automatically– Cumulative and point to point options
• Many Measurement Options– Distance, velocity, etc.
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Tracking
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Tracking
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NEW Acquire Dialog!
• New Basic Capture Dialog– Improved Nomenclature– Vertically aligned to conserve real estate– Most-used controls in one basic dialog
*AND*– AutoSet button – a one-button solution which:
• Resets camera• Performs Auto-White Balance (color only)• Calculates Auto-Exposure• Opens preview pane.
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NEW Acquire Dialog!
Quickly assign and change settings
Lock Preview and Acquire Settings
AutoSet!
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NEW Acquire Dialog!
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Spatial Calibration Improvements
• System Calibration applied when a new image is captured in Image-Pro, Scope-Pro, or AFA
• New Calibration Wizard
• Automatic correction for binning in Scope-Pro
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Spatial Calibration Improvements
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Spatial Calibration Improvements
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New Tools from the Solutions Zone
• Color and orientation are easily chosen
Scale Bar
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Local Zoom
New Tools from the Solutions Zone
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Measurement Overlay
• Allows users to save measurement overlays burned onto an image
Press to snap measurements to image
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Workspace Context Menu Additions- Right-click features
• Local Zoom• Dye Tints• Dye Management
– Allows for custom dyes and for user to define color by emission wavelength
– Pre-Defined Dyes– Add New Dyes– Tint Commands
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Color Composite Improvements
Color Composite Improvements
– Customize Dyes and Colors
– Preset Dye List
– Links to Set and Image Information
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Color Composite Improvements
Press to create new dye
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Color Composite Improvements
Select color by visual inspection or emission wavelength!
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Settings Improvements
• Default Settings Folder– All settings and environment
information is stored in this location
• Lens Information File– Linked to acquisition in Scope-Pro
and AFA
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New Workflow Manager
User-Defined Workflow Toolbar– Use Existing Menu Icons– Create New Icons– Include Self-Contained Macros
without External Parameters
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New Workflow Manager
Sharp Stack 5.0
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Sharp Stack 5.0
• Improved Speed of calculations• Spherical Aberration correction• Included Algorithms
– No Neighbor– Nearest Neighbor– Inverse Filtering
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Sharp Stack 5.0
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Sharp Stack 5.0
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Supplementary AutoQuant Modules
• SharpStack meets the majority market needs
• AutoDeblur Gold WF (widefield fluorescence)
– Contains blind (2D & 3D) deconvolution methods
• AutoDeblur Gold CF (confocal fluorescence)
– Contains confocal deconvolution methods• AutoDeblur Gold WCF (all of the above)
• AutoDeblur 2D (only single plane blind deconvolution)
3D-Constructor 5.0
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3D-Constructor 5.0
• More Visualization effects• Manual Measurements• Automatic Measurements
– 3D Count/Size– Histogram object selection
• 4D Measurements • More Macros
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3D-Pro Series Competition
• Very high prices• Small companies with mixed support
capability• Mixed distribution strategy• Poor dealer relationships• Poor integration into mainstream 2D
image analysis packages
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3D-Pro Series3D Constructor: Volume Rendering
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3D-ConstructorManual Measurements
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3D-ConstructorAutomatic Volumetric Measurements
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3D-ConstructorAutomatic Volumetric Measurements Over Time4D Tracking of objects!!
ScopePro 5.0
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Scope-Pro 5.0 Improvements
• Improved minimal dialogs to save real estate
• Acquire up to 999 Z-stack images • X-Y stage initialization retained
between sessions• Calibration Improvements
– Light path and magnification changers modify calibrations
• Site map for user defined capture regions
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Scope-Pro 5.0
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Scope-Pro 5.0
AFA 4.5Advanced Fluorescence Acquisition
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Advanced Fluorescence Acquisition (AFA) For customers who acquire complex image sets
• Multiple color channels (usually fluorescence) or microscope modes
• Time-lapse, motion studies• Stage position
– Multiwell plates– Area scans– User defined locations
• Multiple focal planes (Z-stack)
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Advanced Fluorescence Acquisition (AFA) Enhanced Capabilities
Time-lapse
Multi-channel Multiwell plates
Z-stack Scan area
User-defined Stage Positions
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Advanced Fluorescence Acquisition (AFA)Interface: First Use
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Advanced Fluorescence Acquisition (AFA)Interface: Preview Tab
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Scope-Pro Device Updates
• Check our website for device updates
Camera Kit Update
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Camera Kit Products
• CoolSNAPcf• Evolution LC• Evolution VF (NEW!)• Evolution MP• Evolution QEi
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CoolSNAP-Pro cf Kits
• 1392 X 1040 pixels• 12-bit chip, Cooled 5C below
ambient• Available in Bayer color or
monochrome or with CRI Micro*Color
• Faster readout speed (20Mhz.)• Preview at 10-12 frames/sec. -
full resolution mono• Significantly Improved
Sensitivity!• Reduced dark current noise
down to <2 electrons!• Supports longer integration for
fluorescence applications
Camera List - $4,999Dealer Discounts up to 36%!
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Evolution LC™ Integrated Camera Kit
• Digital alternative to video microscopy
• Available in monochrome and color
• Features– Fully integrated with your
choice of Image-Pro software– FireWire (IEEE 1394)
C-mount camera– 1.3 megapixel array– Up to 14 fps at 1280 x 1024
res., 30 fps at 640 x 480– Laptop Kit
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Evolution VF - cooled & uncooled version- mono & color
• Features– Designed for high resolution
brightfield scientific and industrial applications.
– A progressive scan interline CCD sensor gives a resolution of 1.4 million pixels in a 12-bit digital output.
– High-speed low noise electronics provide linear digital data
– Frame rates of up to 110 fps in region of interest and binning.
– The IEEE 1394 FireWire™ digital interface allows ease of use
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Evolution VF - Specifications
CCD Sensor
Light Sensitive Pixels 1.4 million, 1392 x 1040
Binning Modes 2x2, 4x4, 8x8
ROI (Region Of Interest)
From 1x1 pixels up to full resolution, continuously variable
Exposure/Integration Control
12µs to 17.9min. in1µs increments
Sensor Type Sony ICX205 Progressive Scan Interline CCD, Monochrome, or Color
Pixel Size 4.65µm x 4.65µm
Linear Full Well 10,000 e-
Read Noise 12 e-
Cooling Available Yes
Cooling Type Peltier Thermoel. cooling 25 degrees Celsius below ambient
Digital Output 12-bit
Readout Frequency 20, 10, 5, 2.5MHz
Frame Rate 10fps full resolution @ 12-bits, 165fps maximum with binning and ROI
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Evolution MP™ Integrated Color Camera Kit
• High resolution 5.5 megapixel color camera
• Features– Fully integrated with your
choice of Image-Pro software– FireWire (IEEE 1394)
C-mount camera– 3-5 fps at 2560x1920(30-bit);
25 fps at 640 x 480– Option for cooling – No interpolation or Pixel
shifting
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Evolution MP™ Integrated Color Camera Kit
Imaging Chip
Type2/3” Sony ICX-282 2 to 1 interlaced
CCD color
Resolution 2588 x 1960
Pixel Size 3.4µm x 3.4µm
Readout Noise 9.8 electrons
Maximum Frame Rate 3-5 fps at 2560 x 1920 (36-bit)
Shutter Electronic shutter, no moving parts
Cooling TypeOptional Peltier thermoelectric
cooling to 10°C below ambient
Physical
Weight 710g
Lens mountC-mount, 2/3” optical format for
microscope or camera lens, plus a 1/4”-20 tripod mount
Interface
Operating System Windows® 98 SE, ME, 2000, XP
Data Format 10-bit
Interface Type IEEE 1394 FireWire
Maximum Data Rate 20 MHz at 8-bit and 10 MHz at 10-bit
Confidential - Do not copy or distribute
Evolution QEi™ Integrated Camera Kit
• High Quantum Efficiency camera• Features
– Fully integrated with your choice of Image-Pro software
– FireWire (IEEE 1394) C-mount camera– 5 fps at 1360x1036(12-bit)10 fps at
1360x1036(8-bit)
Confidential - Do not copy or distribute
Evolution QEi™ Integrated Camera Kit
Imaging Chip
Type2/3” Sony ICX-285 progressive scan
interline CCD monochrome
Resolution 1360 x 1036 Light Sensitive Pixels
Pixel Size 6.45µm x 6.45µm
Readout Noise 8 electrons
Dark Current 0.15 electrons/pixel/sec cooled
Maximum Frame Rate10 fps full resolution in 8-bit, 5fps in 12-
bit. Higher speed with binning and ROI functions (up to 4x4 binning)
Shutter Electronic shutter, no moving parts
Cooling TypePeltier thermoelectric cooling to 25°C
below ambient
Physical
Lens mount C-mount, 2/3” optical format
Interface
Operating System Windows® 98 SE, ME, 2000, XP
Data Format 12-bit
Interface Type IEEE 1394 FireWire
Maximum Data Rate 10 MHz and 20 MHz selectable at 12-bit
IQbaseExtracting Information From Images
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Image-Pro IQbase
• Information and data management software for individuals and companies that need a flexible and powerful database solution
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Image-Pro IQbase
• Object-Oriented Database– More powerful and faster searches than
relational databases found in most competitive products
– Compatible with SQL, Oracle, and other databases
– Can be fully integrated with the world’s leading scientific image analysis software, Image-Pro Plus, which contains numerous input options, measurement and analysis tools, and world-class support
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Image-Pro IQbase - Versatile
• Data can be stored in a variety of locations
• Data is organized according to the users needs and desires
• Saves Time
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Image-Pro IQbase - Versatile
• Can work with MS-SQL, Oracle and other database types
• Data can be made available to web servers for customers/collaborators to view over the world wide web
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Image-Pro IQbase - Expandable
• Suitable for single-user, small workgroup, and enterprise-level deployment
• Seats are easily added• Database can handle virtually
unlimited entries
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Image-Pro IQbase - Intelligent
• Automatically remembers user settings and preferences
• Understands and collects data from within Image-Pro Plus automatically
• Reports can be produced in a variety of formats (PDF, HTML, RTF, etc.)
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Image-Pro IQbase - Programmable
• Includes Visual Basic for Applications®
• Create custom forms, tools and plug-ins from within the product
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IQbase
• Top Features
1. Context Sensitive Searches2. Easy Addition of files to the database3. Image-Pro measurements savable and searchable4. Savable searches5. Multi-user login with optional passwords6. Administration7. Data display8. Report generator produces a variety of formats9. Customizable interface features10. Visual Basic for Applications11. Offline Storage
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Who Will Buy IQbase?
• New Image-Pro customers (plug-in)– Archive measurements automatically
• Existing Image-Pro users– Network installations of IPP
• Near-line storage systems
• Researchers and organizations that need to improve their efficiency
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IQbase
• Database product that includes programming, reporting, and image analysis features– Built for organizations that want to archive their
data and mine it for information at a later time– Perfect for organizations that need to
communicate their findings both internally and externally
– Ideal for those interested in combining powerful data management with image analysis
Future Product Discussions
Business Session
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HEADQUARTERSMedia Cybernetics, Inc.8484 Georgia Avenue, Suite 200Silver Spring, MD 20910 USAPhone: + 1 301 495 3305Fax: + 1 301 495 5964Email: [email protected] Web: www.mediacy.com