Welcome to the Media Cybernetics 2003 Dealer Conference Silver Spring, San Diego, Munich and Singapore August/September 2003

Welcome to the Media Cybernetics 2003 Dealer Conference

Silver Spring, San Diego,

Munich and Singapore

August/September 2003

Confidential - Do not copy or distribute

Introducing Image-Pro Plus 5.0!

• Day One– 9:00AM Welcome & Opening Remarks– 10:00AM-Noon Image Pro Plus Five-0– Noon-1:00PM Lunch– 1:00-2:00PM Image Pro Plus Five-0 (Cont.)– 2:00-3:00PM SharpStack Five-0– 3:00-4:00PM 3D Constructor Five-0– 4:00-5:00PM Scope-Pro Five-0

• Day Two– 9:00-11:00AM What is IQbase?– 11:00-Noon How Can WE Demonstrate & Sell IQbase?– Noon-1:00PM Lunch– 1:00-2:00PM Business Meeting– 2:00PM Adjourn

Agenda for 2-Day Program

Image-Pro Plus 5.0More Answers on your Images!

Image-Pro PlusImage-Pro PlusFive-OFive-O

Image-Pro Plus 5.0

Some Highlights



• Alignment Correction

• Automatic Tiling and Stitching

• Tracking– Automatic and manual– Measurement Display Options

Exciting New Features


• Improved Stack Format Support– Handle stacks better and automatically

assign colors where applicable.

• Color Composite Improvements– Customize Dyes and Colors– Image Set and Existing Image Knowledge

• New Basic Capture Dialog

Introducing Image-Pro Plus 5.0!Exciting New Features



• New Processing and Display Tools– Despeckle Filter– L*A*B Color– Scale Bar– EDF Test Strips

– Local Zoom– Synchronized Playback of

Sequences– Synchronized Pan and Zoom– Surface Plot Overlay from EDF




• Snap to New Image– Allows users to automatically burn

measurement overlays onto an image

• Set Manager– Multidimensional viewer - includes aspects of

the improved sequence file support

• Spatial Calibration Improvements– List Management– System Calibration– Wizard




• Workspace Context Menu Additions– Local Zoom, dye tints, set capture AOI appear in

context menu

• Version Information– Helps tech support know which versions of Image-

Pro Plus and any plug-ins are running

• Default Settings Folder– Location where all settings and environment

information is stored for the product.




• Objective Information File– Important for those that work with microscopes;

links to Scope-Pro and AFA• Display Range/Contrast Enhancement

Interaction– User interface enhancement to make these

tools less independent• Dye Management

– Allows for custom dyes and for user to define color by emission wavelength

– Pre-Defined Dyes– Add New Dyes– Tint Commands




• New Graphical User Interface

• Toolbar Changes– Toolbar has added functionality

• New Menu Structure– Menu is more logical for new users.




• Very New! WorkFlow Manager – User defined function– Toolbar oriented

• Very New! User Identities– Login– Basic, Full, Biological, Industrial defaults


Image-Pro Plus 5.0

Key Selling points

Hands On


IPP 5.0 Alignment Correction

• Align images or a sequence in translation, rotation, and/or scaling.

• Potential applications would be alignment of:– images taken over time with movement– channels of multiple images from a beam

splitter– Serial sections– correcting for offset angle of a Greenough (off-

axis) stereo microscope








• Synchronized Playback of Sequences

• Synchronized Pan and Zoom

Other Tools

New Tools from the Solutions Zone


• EDF Test Strips and EDF Surface Plot

EDF Tools



EDF Tools



Automatic Tiling and Stitching

• Align and stitch images together using a Fourier transform edge detection algorithm based upon both pattern and contrast matching

• Options provide for the insertion of blank images, image order modification, and manual adjustment of pane location

• This will be useful for constructing panoramic views or for linking together multiple images taken with a motorized or non-motorized stage.








Tracking

• Manual Tracking– Click on objects frame by frame

• Automatic Tracking– Highlight objects of interest and let Image-Pro do the

heavy lifting

• Graphs generated automatically– Cumulative and point to point options

• Many Measurement Options– Distance, velocity, etc.


Tracking


Tracking


NEW Acquire Dialog!

• New Basic Capture Dialog– Improved Nomenclature– Vertically aligned to conserve real estate– Most-used controls in one basic dialog

*AND*– AutoSet button – a one-button solution which:

• Resets camera• Performs Auto-White Balance (color only)• Calculates Auto-Exposure• Opens preview pane.


NEW Acquire Dialog!

Quickly assign and change settings

Lock Preview and Acquire Settings

AutoSet!


NEW Acquire Dialog!


Spatial Calibration Improvements

• System Calibration applied when a new image is captured in Image-Pro, Scope-Pro, or AFA

• New Calibration Wizard

• Automatic correction for binning in Scope-Pro







• Color and orientation are easily chosen

Scale Bar


Local Zoom



Measurement Overlay

• Allows users to save measurement overlays burned onto an image

Press to snap measurements to image


Workspace Context Menu Additions- Right-click features

• Local Zoom• Dye Tints• Dye Management

– Allows for custom dyes and for user to define color by emission wavelength

– Pre-Defined Dyes– Add New Dyes– Tint Commands


Color Composite Improvements


– Customize Dyes and Colors

– Preset Dye List

– Links to Set and Image Information



Press to create new dye



Select color by visual inspection or emission wavelength!


Settings Improvements

• Default Settings Folder– All settings and environment

information is stored in this location

• Lens Information File– Linked to acquisition in Scope-Pro

and AFA


New Workflow Manager

User-Defined Workflow Toolbar– Use Existing Menu Icons– Create New Icons– Include Self-Contained Macros

without External Parameters


New Workflow Manager

Sharp Stack 5.0


Sharp Stack 5.0

• Improved Speed of calculations• Spherical Aberration correction• Included Algorithms

– No Neighbor– Nearest Neighbor– Inverse Filtering


Sharp Stack 5.0


Sharp Stack 5.0


Supplementary AutoQuant Modules

• SharpStack meets the majority market needs

• AutoDeblur Gold WF (widefield fluorescence)

– Contains blind (2D & 3D) deconvolution methods

• AutoDeblur Gold CF (confocal fluorescence)

– Contains confocal deconvolution methods• AutoDeblur Gold WCF (all of the above)

• AutoDeblur 2D (only single plane blind deconvolution)

3D-Constructor 5.0


3D-Constructor 5.0

• More Visualization effects• Manual Measurements• Automatic Measurements

– 3D Count/Size– Histogram object selection

• 4D Measurements • More Macros


3D-Pro Series Competition

• Very high prices• Small companies with mixed support

capability• Mixed distribution strategy• Poor dealer relationships• Poor integration into mainstream 2D

image analysis packages


3D-Pro Series3D Constructor: Volume Rendering


3D-ConstructorManual Measurements


3D-ConstructorAutomatic Volumetric Measurements


3D-ConstructorAutomatic Volumetric Measurements Over Time4D Tracking of objects!!

ScopePro 5.0


Scope-Pro 5.0 Improvements

• Improved minimal dialogs to save real estate

• Acquire up to 999 Z-stack images • X-Y stage initialization retained

between sessions• Calibration Improvements

– Light path and magnification changers modify calibrations

• Site map for user defined capture regions


Scope-Pro 5.0


Scope-Pro 5.0

AFA 4.5Advanced Fluorescence Acquisition


Advanced Fluorescence Acquisition (AFA) For customers who acquire complex image sets

• Multiple color channels (usually fluorescence) or microscope modes

• Time-lapse, motion studies• Stage position

– Multiwell plates– Area scans– User defined locations

• Multiple focal planes (Z-stack)


Advanced Fluorescence Acquisition (AFA) Enhanced Capabilities

Time-lapse

Multi-channel Multiwell plates

Z-stack Scan area

User-defined Stage Positions


Advanced Fluorescence Acquisition (AFA)Interface: First Use


Advanced Fluorescence Acquisition (AFA)Interface: Preview Tab


Scope-Pro Device Updates

• Check our website for device updates

Camera Kit Update


Camera Kit Products

• CoolSNAPcf• Evolution LC• Evolution VF (NEW!)• Evolution MP• Evolution QEi


CoolSNAP-Pro cf Kits

• 1392 X 1040 pixels• 12-bit chip, Cooled 5C below

ambient• Available in Bayer color or

monochrome or with CRI Micro*Color

• Faster readout speed (20Mhz.)• Preview at 10-12 frames/sec. -

full resolution mono• Significantly Improved

Sensitivity!• Reduced dark current noise

down to <2 electrons!• Supports longer integration for

fluorescence applications

Camera List - $4,999Dealer Discounts up to 36%!


Evolution LC™ Integrated Camera Kit

• Digital alternative to video microscopy

• Available in monochrome and color

• Features– Fully integrated with your

choice of Image-Pro software– FireWire (IEEE 1394)

C-mount camera– 1.3 megapixel array– Up to 14 fps at 1280 x 1024

res., 30 fps at 640 x 480– Laptop Kit


Evolution VF - cooled & uncooled version- mono & color

• Features– Designed for high resolution

brightfield scientific and industrial applications.

– A progressive scan interline CCD sensor gives a resolution of 1.4 million pixels in a 12-bit digital output.

– High-speed low noise electronics provide linear digital data

– Frame rates of up to 110 fps in region of interest and binning.

– The IEEE 1394 FireWire™ digital interface allows ease of use


Evolution VF - Specifications

CCD Sensor

Light Sensitive Pixels 1.4 million, 1392 x 1040

Binning Modes 2x2, 4x4, 8x8

ROI (Region Of Interest)

From 1x1 pixels up to full resolution, continuously variable

Exposure/Integration Control

12µs to 17.9min. in1µs increments

Sensor Type Sony ICX205 Progressive Scan Interline CCD, Monochrome, or Color

Pixel Size 4.65µm x 4.65µm

Linear Full Well 10,000 e-

Read Noise 12 e-

Cooling Available Yes

Cooling Type Peltier Thermoel. cooling 25 degrees Celsius below ambient

Digital Output 12-bit

Readout Frequency 20, 10, 5, 2.5MHz

Frame Rate 10fps full resolution @ 12-bits, 165fps maximum with binning and ROI


Evolution MP™ Integrated Color Camera Kit

• High resolution 5.5 megapixel color camera

• Features– Fully integrated with your

choice of Image-Pro software– FireWire (IEEE 1394)

C-mount camera– 3-5 fps at 2560x1920(30-bit);

25 fps at 640 x 480– Option for cooling – No interpolation or Pixel

shifting


Evolution MP™ Integrated Color Camera Kit

Imaging Chip

Type2/3” Sony ICX-282 2 to 1 interlaced

CCD color

Resolution 2588 x 1960


Readout Noise 9.8 electrons

Maximum Frame Rate 3-5 fps at 2560 x 1920 (36-bit)

Shutter Electronic shutter, no moving parts

Cooling TypeOptional Peltier thermoelectric

cooling to 10°C below ambient

Physical

Weight 710g

Lens mountC-mount, 2/3” optical format for

microscope or camera lens, plus a 1/4”-20 tripod mount

Interface

Operating System Windows® 98 SE, ME, 2000, XP

Data Format 10-bit

Interface Type IEEE 1394 FireWire

Maximum Data Rate 20 MHz at 8-bit and 10 MHz at 10-bit


Evolution QEi™ Integrated Camera Kit

• High Quantum Efficiency camera• Features

– Fully integrated with your choice of Image-Pro software

– FireWire (IEEE 1394) C-mount camera– 5 fps at 1360x1036(12-bit)10 fps at

1360x1036(8-bit)


Evolution QEi™ Integrated Camera Kit

Imaging Chip

Type2/3” Sony ICX-285 progressive scan

interline CCD monochrome

Resolution 1360 x 1036 Light Sensitive Pixels


Readout Noise 8 electrons

Dark Current 0.15 electrons/pixel/sec cooled

Maximum Frame Rate10 fps full resolution in 8-bit, 5fps in 12-

bit. Higher speed with binning and ROI functions (up to 4x4 binning)

Shutter Electronic shutter, no moving parts

Cooling TypePeltier thermoelectric cooling to 25°C

below ambient

Physical

Lens mount C-mount, 2/3” optical format

Interface

Operating System Windows® 98 SE, ME, 2000, XP

Data Format 12-bit

Interface Type IEEE 1394 FireWire

Maximum Data Rate 10 MHz and 20 MHz selectable at 12-bit

IQbaseExtracting Information From Images


Image-Pro IQbase

• Information and data management software for individuals and companies that need a flexible and powerful database solution


Image-Pro IQbase

• Object-Oriented Database– More powerful and faster searches than

relational databases found in most competitive products

– Compatible with SQL, Oracle, and other databases

– Can be fully integrated with the world’s leading scientific image analysis software, Image-Pro Plus, which contains numerous input options, measurement and analysis tools, and world-class support


Image-Pro IQbase - Versatile

• Data can be stored in a variety of locations

• Data is organized according to the users needs and desires

• Saves Time


Image-Pro IQbase - Versatile

• Can work with MS-SQL, Oracle and other database types

• Data can be made available to web servers for customers/collaborators to view over the world wide web


Image-Pro IQbase - Expandable

• Suitable for single-user, small workgroup, and enterprise-level deployment

• Seats are easily added• Database can handle virtually

unlimited entries


Image-Pro IQbase - Intelligent

• Automatically remembers user settings and preferences

• Understands and collects data from within Image-Pro Plus automatically

• Reports can be produced in a variety of formats (PDF, HTML, RTF, etc.)


Image-Pro IQbase - Programmable

• Includes Visual Basic for Applications®

• Create custom forms, tools and plug-ins from within the product


IQbase

• Top Features

1. Context Sensitive Searches2. Easy Addition of files to the database3. Image-Pro measurements savable and searchable4. Savable searches5. Multi-user login with optional passwords6. Administration7. Data display8. Report generator produces a variety of formats9. Customizable interface features10. Visual Basic for Applications11. Offline Storage


Who Will Buy IQbase?

• New Image-Pro customers (plug-in)– Archive measurements automatically

• Existing Image-Pro users– Network installations of IPP

• Near-line storage systems

• Researchers and organizations that need to improve their efficiency


IQbase

• Database product that includes programming, reporting, and image analysis features– Built for organizations that want to archive their

data and mine it for information at a later time– Perfect for organizations that need to

communicate their findings both internally and externally

– Ideal for those interested in combining powerful data management with image analysis

Future Product Discussions

Business Session


HEADQUARTERSMedia Cybernetics, Inc.8484 Georgia Avenue, Suite 200Silver Spring, MD 20910 USAPhone: + 1 301 495 3305Fax: + 1 301 495 5964Email: [email protected] Web: www.mediacy.com

Documents

Welcome to the Media Cybernetics 2003 Dealer Conference Silver Spring, San Diego, Munich and Singapore August/September 2003