Top producing beamlines of the world
0
20
40
60
80
100
120
140
160
180
AP
S 19ID
AL
S 8.3.1
ES
RF
ID14-1
ES
RF
ID14-4
PF
(all 5)
ES
RF
ID14-2
NS
LS
X4A
AP
S 22ID
ES
RF
ID29
AP
S 19B
M
CH
ES
S (all 4)
AL
S 8.2.1
AL
S 5.0.2
HA
SY
LA
B X
11
HA
SY
LA
B B
W6
AL
S 8.2.2
SL
S (b
oth
)
ES
RF
BM
14
SP
RIN
G8 26B
1
AP
S 14B
MC
NS
LS
X25
AL
S 5.0.1
SS
RL
BL
9-1
NS
LS
X12C
20022003
2004
http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html
Str
uct
ure
s cr
edit
ed
Top producing beamlines of the world
0
0.51
1.5
22.5
3
3.54
4.5
5
AP
S 19ID
ES
RF
ID14-4
ES
RF
ID14-1
ES
RF
ID29
HA
SY
LA
B B
W6
AL
S 8.3.1
ES
RF
ID14-2
AP
S 22ID
AP
S 14B
MC
SL
S X
06SA
AL
S 8.2.1
AP
S 19B
M
NS
LS
X4A
SS
RL
BL
9-1
HA
SY
LA
B X
11
AL
S 8.2.2
SR
S 14.2
NS
LS
X25
SP
RIN
G8 26B
1
ES
RF
BM
14
AL
S 5.0.2
SS
RL
BL
11-1
MA
XL
AB
I711
NS
LS
X12C
NS
LS
X8C
20022003
2004
http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html
x106
un
iqu
e H
KL
s
Top producing beamlines of the world
0
200
400
600
800
1000
1200
1400
1600
1800
2000
NS
LS
X26C
NS
LS
X12C
AP
S 14B
MD
NS
LS
X8C
NS
LS
X4A
NS
LS
X12B
AL
S 5.0.1
NS
LS
X9B
AL
S 5.0.3
SS
RL
BL
9-1
AP
S 14B
MC
SS
RL
BL
7-1
AL
S 5.0.2
SS
RL
BL
1-5
AL
S 8.3.1
SS
RL
BL
9-2
AL
S 8.2.1
SS
RL
BL
11-1
AP
S 14ID
B
NS
LS
X25
AP
S 19B
M
AL
S 8.2.2
CH
ES
S F
2
CH
ES
S F
1
CH
ES
S A
1
AP
S 17B
M
NS
LS
X9A
AP
S 17ID
AP
S 19ID
20022003
2004
http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html
http://biosync.sdsc.edu/
Str
uct
ure
s/10
20 p
ho
ton
s
Number Description Percent
446028 Images (~7 TB) 33%
2346 Data sets 47%
449 MAD/SAD (1:2) 19%
48 Published 2%
8.3.1 in 2003
Beamline Efficiency
Seconds Description Percent
104490 Assigned and available 91%
42093 Shutter open 40%
52684 Collecting (3026 images) 50%
51806 Something else 50%
Beamline Efficiency“representative” 8.3.1 user
Seconds Description Percent
51806 Something else 100%
247s 45
Mounting 22%
229s 37
Centering 16%
179s109
Strategizing 38%
309s 37
Prepping 24%
Beamline Efficiency“representative” 8.3.1 user
Seconds Description Percent
16820 Something else 100%
10s 45 Mounting 3%
30s 37 Centering 7%
140s109
Strategizing 90%
0s 37 Prepping 0%
Beamline Efficiency“expert” user
Touch screen
Traffic light
firewire
DVD data archive
Beamline pager
Ice-B-gone
Foam dewars
Foam dewars
Zero-parallax optics
pinhole
prism
microscope
backstop
Zero-parallax optics
pinhole
prism
microscope
backstop
Zero-parallax optics
pinhole
prism
microscope
Styrofoam™ backlight
backstop
Zero-parallax optics
pinhole
prism
microscope
Apr 6 – 24 at ALS 8.3.1
Elven Automation
27,686 images collected
148 datasets (15 MAD)
31 investigators
56 unique cells
5 KDa – 23 MDa asymmetric unit
0.94 – 32 Å resolution (3.2 Å)
Apr 6 – 24 at ALS 8.3.1
Elven Automation
148 datasets
117 succeded
~3.5 (0.1-75) hours
31 failed
~61 (0-231) hours
2 / 15 MAD structures
Number Description Percent
446028 Images (~7 TB) 33%
2346 Data sets 47%
449 MAD/SAD (1:2) 19%
48 Published 2%
8.3.1 in 2003
Beamline Efficiency
Hampton Pin
Syrrx Pin
plastic Pin
Yale Pin
SuperPin
SuperTongSuper Tong
Hampton PinSuper Tong
Syrrx PinSuper Tong
plastic PinSuper Tong
Yale PinSuper Tong
SuperTong
thaw
Radiation Damage
Incremental strategy
incremental_strategy.com merged.mtz auto.mat
Incremental strategy
incremental_strategy.com merged.mtz auto.mat
OTIS
shoot nextsample
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
Expe
rim
ent
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA
using 1 Å x-rays detector at 2 Å resolution
OTIS
recentered data set from 50º to 150º with 1º oscillations using 1 Å x-rays detector at 2.5 Å resolution