The skin cohesiondesigner
SqisandrylTM
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Overview
Efficacy
Plant origin–
Ecocertcertified
Regulatory
Extraction process
Scientificstory
Sqisandryl™ concept&
Efficacy
Our value proposition
Technicaldata
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Sqisandryl™ concept&
Efficacy
Our value proposition
Technicaldata
Overview
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Sqisandryl™ concept
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With Sqisandryl™
Dermal-Epidermal
Junction
FOCUS on
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With Sqisandryl™
Collagen XVIILadinin-1
Stimulation of collagen XVII and ladinin-1 synthesis
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Collagen XVII and Ladinin-1
Ladinin-1
Play a major role in cohesion & tensionbetween dermis & epidermis,Involved in deseases when severely lacking.
Collagen XVIISynthesized by
EpidermalKeratinocytes
2 “tension molecules”
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Definition: structural component of the dermo-epidermal anchoring complex
Location: DEJ (Lumina lucida)
Main function: cell-matrix adhesion molecule throughstabilization of the hemidesmosome complex.
Collagen XVII: collagenous transmembrane protein
Powell AM, et al., 2005, Collagen XVII/BP180: a collagenous transmembrane protein and component of the dermo-epidermal anchoring complex, Exp. Dermatol., 30:682-687
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Definition: anchoring filament that is a component of basement membranes
Location: synthesized and secreted by epidermal keratinocytes DEJ (Lumina lucida)
Main function: may contribute to the stability of the association of the epithelial layers with the underlying connective tissue
Ladinin-1: component of basement membranes
El Ghalbzouri A, et al.: Basement membrane reconstruction in human skin equivalents is regulated by fibroblasts and/or exogen activated keratinocytes, JID., 124:79-86, 2005
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As we aged…
Keratinocytes metabolism slows down
1
Coll. XVII and Ladinin-1
proportions are expected to decrease
2
SKIN SLACKENING,
LOSS OF FIRMNESS
No more dermal-epidermal tension,
DEJ deformation
3
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With Sqisandryl™…
Stimulates Collagen XVII and Ladinin-1 synthesis
Dermal-epidermal dynamic cohesion is
recoveredNEW
Consumer Benefits
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NEW CLAIMS related to DEJ on the market
Beyond wrinkles or firmness correction and moisturization…
…Sqisandryl™ improves
simultaneous
ELASTICITYRESILIENCE
FIRMNESS
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Sqisandryl™
Efficacy
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Sqisandryl™
In vitro results
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Collagen XVII stimulation
In vitro
On keratinocytes culture
Expression of the COL17A1 gene detected and quantified by qRT-PCR
The collagen XVII protein is detected by immunocytochemistry
+67%
6×
SqisandrylTM increases
Collagen XVII synthesis up to 6 times
Col17A1 mRNA expression by 67%
Mean of 4 assays – U test of Mann and Whitney – *: p<0.05 Mean of 3 assays – U test of Mann and Whitney – **: p<0.01
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Control SQISANDRYL™ at 0.006% SQISANDRYL™ at 0.01%
In vitro
On keratinocytes culture The collagen XVII protein is quantified by image analysis
Collagen XVII stimulation
Image analysis
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In vitro
On keratinocytes culture Expression of the LAD-1 gene detectedand quantified by qRT-PCRThe ladinin-1 protein is detected by immunocytochemistry.
LAD-1 mRNA expression by 68%
+68%
Ladinin-1 stimulation
Sqisandryl™ increases
3.5×
Ladinin-1 synthesis up to 3.5 times
Mean of 5 assays – U test of Mann and Whitney – **: p<0.01 Mean of 3 assays – U test of Mann and Whitney – **: p<0.01
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In vitro
On keratinocytes culture The ladinin-1 protein is quantified by image analysis
Control SQISANDRYL™ at 0.006% SQISANDRYL™ at 0.01%
Ladinin-1 stimulation
Image analysis
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Sqisandryl™
The clinical study
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1 group of 17 subjects women over 60 with a slackening of elasticity and firmness on the face. 8 weeks of treatment, half face application twice a day.
Evaluation of Skin biomechanical properties measurements over time with Cutometer® at D0, D56.
Protocol
Sqisandryl™ at 0.3%*
Placebo*
* Hydrogel formulation
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Skin elasticity
After 56 days,skin elasticity is
improved by 28%
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Skin resilience
After 56 days,resilience is
improved by 8%
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Skin firmness
After 56 days, skin firmness is improved
by 25%
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Overview
Efficacy
Plant origin–
Ecocertcertified
Regulatory
Extraction process
Scientificstory
Sqisandryl™ concept&
Efficacy
Our value proposition
Technicaldata
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Our value proposition
Efficacy
Plant origin–
Ecocertcertified
Regulatory
Extraction process
Scientificstory
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26
Our value proposition
Northern China RussiaKorea
Geographic
origin
“five-flavor berries”běi wǔ wèi zi (Mandarin)Omicha (Korean)Magnolia vine
Com
mon nam
esPlant
origin
–
Ecocert
certified
Schisandra chinensisberry
For 2000 years, one of the 50 important Chinese herbal
medecineHis
tory
Help to beautify the skinMaintain the body’s tonicity
Improve concentration and attentionIncrease physical and mental performance
Prevent and treate heart diseases
Trad
ition
al u
ses
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Our value proposition
Extraction process
Supercritical CO2extraction advantages:
- safe and clean,
- allows the complete elimination of solvent,
- remarkable solvent properties, non-destructive,
- confers a unique phytochemical profile on Sqisandryl™
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Our value proposition
RegulatoryAll the raw materials comprising the INCI name SqisandrylTM are listed on the "International Cosmetic Ingredient Standard Chinese Name"(2007 version)
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Our value proposition
Scientificstory
Efficacy
Identification of 2 “tension molecules” of the DEJ:
- collagenXVII,- ladinin-1.
Create a NEW s c i e n t i f i c s t o r y about the DEJ.No cosmetic active ingredient on the market with these targets.
Sqisandryl™ reinforces the dynamic cohesionbetween the dermis and the epidermis.
The skin is more elastic, more resilient and firmerin appearance in only 8 weeks.
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Overview
Sqisandryl™ concept&
Efficacy
Our value proposition
Technicaldata
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Process: Supercritical CO2 extraction (innovating and eco friendly process)
Aspect: yellow to orange oily liquid, with characteristic odor
Dose of use: 0.1 – 0.5% (recommended 0.3%, skin care applications)
Solubility: soluble in oils and fats
Preservatives: none
Ecocert: Raw material certified by Ecocert Greenlife according to the Ecocert Standard for Natural and Organic Cosmetics available at http://cosmetics.ecocert.com: 100% of the total ingredients are from natural origin, 100% of the total ingredients are from Organic Farming.
SqisandrylTM LS 9905Technical data
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INCI Name (EU/US): Schizandra Chinensis Fruit Extract
CAS number: 223748-53-6
Patent application WO 2005/044289 A2: EP (DE, FR, ES, IT, GB), US, JP
Japanese regulation: Cannot be used for cosmetic product in Japan
Chinese regulation:
SqisandrylTM LS 9905Regulatory data
MOH 2003 (1) INCI 2007 (2) INCI 2010 (3)
(1) INCI name/plant name whether or not listed in 'MOH Approved Used Cosmetic Ingredients (2003)(2) INCI name whether or not listed in "International Cosmetic ingredient standard Chinese name"(2007 version)(3) INCI name whether or not listed in "International Cosmetic ingredient standard Chinese name"(2010)
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SqisandrylTM LS 9905Toxicological/Safety data
EPISKIN: skin irritation in vitro
BCOP: eye irritation in vitro
IMDS: the modified Local Lymph Node Assay (sensibilization on animal)
AMES: Salmonella typhimurium Reverse Mutation Assay (in vitro mutagenicity )
Phototoxicity: in vitro
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Oil solubleSuitable pH: 4-8
Hydrogel formulation with 0.3%
Sqisandryl™
O/W cream formulation with 0.3% Sqisandryl™
SqisandrylTM LS 9905Formulation
Easy to formulate in all types of formulation
Low impact (color/odor)
in formulation at 0.3%
The active ingredient Sqisandryl™
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From traditional use… 3 NEW consumer benefits:ELASTICITYRESILIENCEFIRMNESS
…to NEW cosmetic application.
Sqisandryl™The Skin Cohesion Designer
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Sqisandryl™The Skin Cohesion Designer
Reinforcement of the dynamic cohesions between the dermis
and the epidermis.
Stimulation of collagen XVII and ladinin-1 synthesis,
two “tension molecules” of the DEJ
Remodeling face care
Skin restructuring treatments
Skin repair elixir
Make-up (foundation, lipstick)
PRO
PERT
IES
APP
LICA
TIO
NS
Men and women + 45 yo
TARG
ETED
CO
NSU
MER
S
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Disclaimer
“Although all statements and information in this publication are believed to be accurateand reliable, they are presented gratis and for guidance only, and risks and liability forresults obtained by use of the products or application of the suggestions described areassumed by the user. SELLER MAKES NO WARRANTY OF ANY KIND, EITHEREXPRESS OR IMPLIED, BY FACT OR LAW, INCLUDING WARRANTIES OFMERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Statements orsuggestions concerning possible use of the products are made without representationor warranty that any such use is free of patent infringement and are notrecommendations to infringe any patent. The user should not assume that toxicity dataand safety measures are indicated or that other measures may not be required. Theclaims and supporting data provided in this publication have not been evaluated forcompliance with any jurisdiction’s regulatory requirements and the results reportedmay not be generally true under other conditions or in other matrices. Users mustevaluate what claims and information are appropriate and comply with a jurisdiction’sregulatory requirements. Recipient of this publication agrees to (i) indemnify and holdharmless each entity of the BASF organization for any and all regulatory action arisingfrom recipient’s use of any claims or information in this publication, including, but notlimited to, use in advertising and finished product label claims, and (ii) not present thispublication as evidence of finished product claim substantiation to any regulatoryauthority.”