Download pdf - minoTour: real-time data analysis for nanopore sequence data...minoTour includes a simple to use GUI to easy configuration and uploading of sequencing run data in real time into the

minoTour includesasimpletouseGUItoeasyconfigurationanduploadingof sequencingrundatainrealtimeintotheminoTourmySQLdatabase

1.ABSTRACT 2.GraphicalUserInterface

9.Availability

4.PoreActivityandReadQuality

3.RunSummaries– ReadNumbersandRates 5.AlignmentstoReferenceGenome

7.Anytime,anywhere…

minoTour:real-timedataanalysisfornanoporesequencedata

MartinBlythe,SunirMalla,MichaelStoutandMatthewLooseDeepSEQ,SchoolofLifeSciences,

UniversityofNottingham,Nottingham,NG72UH

8.AskforaDemo@LC2016!

minoTour nowincludes asimpletouseGUItoeasyconfiguration andupload ofsequencingrundataintotheminoTourmySQLdatabase

Othernewfeaturesinclude batchingofBWAalignmentsresultinginsignificantspeedimprovements

minoTour isaplatformforreal-timemonitoring,analysisandinterpretationofsequencedatastreamedfromtheOxford NanoporeminION sequencing device,includingmetricsdescribingsequence qualityandalsoalignmenttoareferencegenomewhereappropriate.minoTourcomprises aWebBrowserinterfaceintoarelationaldatabaseofminIONsequencing datacapturedinreal-timeandincludesaGraphicalUserInterfacetoassistconfigurationofdataupload.minoTour cananalysedatabothpreandpostbasecalling,canremotelynotifyusersofkeyeventsduringsequencing andexploitnovelsequencing featuresmadepossibleforthefirsttimebyminIONdevicesincludingdirectcontrolofthedeviceinreal-time;enabling‘rununtil’experiments.minoTourisopen sourcesoftware,freelyavailableonallmajoroperatingsystemandcommunitydevelopmentisencouraged. AtLondonCalling2016wewilldemonstrateminoTourmonitoringandanalysingminIONsequencing andcontrollingtheminIONsequencer inreal-time.

Awiderangeofsummaryinformation abouteachsequencing runisavailableastherunisprogressing,includingstatisticsonnumbersof andlengthsofreadsalongwithsequencing rates.

minoTour enablesnanoporeactivitiesandreadqualitytobemonitoredinreal-time

UserscansetupTwitteralertsforimportantruneventssuchaswhenaspecific coveragelevelisreached

minoTour cangeneratesummaryplotsformapping readstoareferencegenome usingreadsthathavebeenbasecalledusingmetricore.

Rawpre-basecalled readscanalsobealignedusingdynamictimewarpingofreadeventsdataagainstaputativereferenceeventtrace

6.RemotecontrolofminIONruns

Previoussequencing runscanbestored,managedandarchived,andreadsviewedandexportedasfasta,fastqorSAMfilesfromminoTour’s easytousewebinterface

minoTour canremotelycontroltheminION,startingandstoppingruns,renamingexperiments, andevenmonitoringchannel stateswhilstrunsareprogressing

ResponsivewebtechnologyenablestominoTourwebservicetobeaccessed fromPCs,laptops,tabletsandsmartphones

minoTour canbedownloaded fromhttps://github.com/minoTour/minoTourAhostedminoTourserviceisalsoavailableathttp://minotour.nottingham.ac.uk/[email protected]:Realtimeselectivesequencingusingnanoporetechnology.MatthewLoose,SunirMalla,MichaelStout.BioRXivFeb2016