M. truncatula

Control (h)GSH-depleted

BSO treatment

Inoculation with S. meliloti

Time course (0, 12, 24,…, 96h)

cDNA-AFLP analysis6149 tags visualized

Data quantification and normalization

Time course (0, 12, 24,…, 96h)

Group A:299 nodulation

regulated genes

Control/(h)GSH-depleted variance test p<0.05

Group B:181 (h)GSH-regulated genes

CVnod ≥ 0.4

Figure S1: Experimental design and data analysis scheme for isolation of (h)GSH-regulated genes during the nodulation process. Group A: the Coefficient of Variance (CVnod) ≥ 0.4, was retained to select genes differentially expressed during the nodulation process. Group B : analysis of variance (P≤0.05), was performed to select genes affected by the (h)GSH depletion