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M. truncatula. Control. cDNA-AFLP analysis 6149 tags visualized. Group A: 299 nodulation regulated genes. CVnod ≥ 0.4. Control/(h)GSH-depleted variance test p
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M. truncatula
Control (h)GSH-depleted
BSO treatment
Inoculation with S. meliloti
Time course (0, 12, 24,…, 96h)
cDNA-AFLP analysis6149 tags visualized
Data quantification and normalization
Time course (0, 12, 24,…, 96h)
Group A:299 nodulation
regulated genes
Control/(h)GSH-depleted variance test p<0.05
Group B:181 (h)GSH-regulated genes
CVnod ≥ 0.4
Figure S1: Experimental design and data analysis scheme for isolation of (h)GSH-regulated genes during the nodulation process. Group A: the Coefficient of Variance (CVnod) ≥ 0.4, was retained to select genes differentially expressed during the nodulation process. Group B : analysis of variance (P≤0.05), was performed to select genes affected by the (h)GSH depletion