How to Screen out the High Productive Algae?
Qian Zhou10/16/2009
What is the criteria?• Daily biomass?• Nile Red Staining?• Biomass carbon and nitrogen percentage?• Daily lipids?• Daily carbon?• Daily reduced carbon?• (Batch)Total biomass in a relatively shot period?• (Batch)Total lipids in a relatively shot period?• Photosynthetic efficiency?• Nitrogen effects?
EV-B-6 EV-B-5 EV-B-1 EV-OR-2
EV-OR-5
EV-OR-1
EV-OR-6
EV-B-2 EV-B-7 EV-B-8 MCI PF-30
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Series1
Figure1: daily biomass production of 12 algae stains
What we have done?
Method of lipid determination
• Gas Chromatography• Nile Red Staining• TD-NMR/ Regular NMR
TD-NMR
• Time domain NMR is an alternative method for lipid analysis.
• Highly accurate, no pretreatment needed• Could determine the lipid concentration in
real- time
basic principles of td-NMR
Plans for next step
• Determine the daily total biomass and lipids of 6~8 species out of 30 strains
• Determine the Phi of the 6 speciesⅡ• Determine the carbon dioxide utilization
of these species• Determine the nitrogen utilization of these
species• Compare FAME analysis with NMR data
Thank you!