Drug delivery related to metabolites in human breath – fi rst results of long term clinical studies using MCC/IMS

* Korea Ins tute of Science and Technology Europe Forschungsgesellscha mbH (KIST Europe)Campus E 7 1 - 66123 Saarbrücken - Germany

phone + 49 (0) 681 9382 229 - fax + 49 (0) 681 9382 240

h p://www.kist-europe/r_cd_jibb.htmlh p://www.kist-europe.comh p://www.kist-europe.de

Contact: PD Dr. Jörg Ingo Baumbach Head of Department Microfl uidics and Clinical Diagnos cs baumbach@kist-europe.de

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Part of a IMS-Chromatogram of human breath

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ContaContaContaContaContaonontaContaontontontContaontContaonContaContaContaContaoonttnoo ct:ct:ct:ct:ctcctct:ct:ct:tct PDPDPDPDPDPDPDDPDDPDDPDPDPDPPDPPDDDP Dr JDr. JDr. JDrDr.Drr. Jr Jr. JJJJDr. JDDr.r. JDr. JJrD JDD örg Iörg Iörg Iörg Iörg örgörg Iörg örg Iörg Iörg Iörg Iörg Iörg Iörg örg örg Irg Irgg örg Iörg Iör ngo Bngo Bngo Bgo Bgoggo Bgo Bgo Bngo Bngo Bgo Bgo Bgo Bgo Bgo Boo Bgo Bgo Bgo Bngo gogo Bngo Bgo ngo on aumaumbaaumbaaumbaaumbaaumbaaumbaaumbaumbauaumbaumbaaumbaaumbaumbaumaumbambaumbaaumbaaumbbauaumbmbaumbmbaaa bu am chchchchcchchchchchcchccchchchhddddd fff flflflflfl dddddddddd ddd lll lll

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M. Westhoff 1, P. Li erst1, S. Maddula2, J.I. Baumbach2,3

1 Lung Clinic Hemer, Theo-Funccius-Str. 1, 58675 Hemer, Germany2 KIST Europe, Department Microfl uidics and Clinical Diagnos cs, Campus E 71, 66123 Saarbrücken, Germany

3 B&S Analy k, BioMedicalCenter Dortmund, O o-Hahn-Str. 15, 44227 Dortmund, Germany

Acknowledgements

The fi nancial support of the Ministry of Educa on, Science and Technology (MEST) of the Republic

Korea is acknowledged thankfully. Part of the work on this paper has been supported by Deutsche For-schungsgemeinscha (DFG) within the Collabora -ve Research Center (Sonderforschungsbereich) SFB 876 „Providing Informa on by Resource-Constrai-ned Analysis“, project TB1 “Resource-Constrained

Analysis of Spectrometry Data“.

In addi on, the work was supported partly by the German Federal Ministry of Economics and Tech-

nology based on a decision of the German Bundes-tag within the project KF2368102AKO.

The experimental work described was carried out partly at ISAS Dortmund, Germany.

0 62 124 186 24840

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0 62 124 186 248

0,002

0,004

0,006

0,008

0,010

0,012

0,014

0,016

GAG

GA

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a.u.

IMS - Peak height

Sig

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t / a

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Time / d

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Peak P-77.2/0.601

IMS

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40

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GA

G /

a.u

d

The concentra ons were higher than the concentra ons of

all pa ents ever tested with MCC/IMS. Furthermore, a peak P-77.2/0.601 was found,

which decreased a er few weeks of therapy and needs further biochemical characterisa on.

Parallel GC/MSD inves ga ons suggest (S)-(+)-6-Methyl-1-octanol[110453-78-6], Cyclohexanone, 5-methyl-2-(1-methylethyl)- [10458-14-7], Benzaldehyde, 2,5-dimethyl-

[5779-94-2], Ethanone, 1-(4-methylphenyl)- [122-00-9], Dodecane [112-40-3] or Decanal [112-31-2].

These preliminary data show that breath analysis in pa ents with metabolic disorders might give

further informa on about metabolism, especially under enzyme replace-

ment therapy.

Pa ent Father Mother

GAG

PartPartPartPartPartartr

PPa ePa ePa ePa ePa eeeentntntntnnn

Hunter disease is a mucopolysaccharidosis for

which enzyme replacement therapy with idursulfase has been off ered for 3 ye-

ars. Therapy can be monitored by the urinary concentra on of dermatan sulfate and heparin sul-fate. A 38-year-old male person suff ering from Hun-ter disease has been treated with idursulfase for a year, showing drama c clinical improvement. Addi onally,

the pa ent had breath analysis using MCC/IMS every week before enzyme replacement. The

pa ent showed increasing concentra ons of a peak P-1.5/0.547 which is rela-

ted to acetone [67-64-1] over the me.

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