Centro de Investigaciones en Enfermedades Infecciosas
Figure 1. Ocular OI were clinically assessed. A. CMVr retinitis;
B., ocular syphilis with severe vitritis (seric VDRL+).
A B
R E S U LT S
Opportunist ic ocular infect ions (OI) are not uncommon in HIV
infect ion. Ocular immune response in the aqueous humor (AqH) has
many unique propert ies that are st i l l under research.
Understanding these patterns could lead to improved or novel t
reatments of ocular OI. One previous study1 found a predominant ly
Th1 response in AqH of CMVr. Using a multiplex technology, we
measured mult ip le cytokine and chemokine prof i les in AqH and
plasma of HIV- infected indiv iduals wi th OI, before and af ter
speci f ic t reatment.
Cytokine Profile in Aqueous Humor of HIV Patients with Ocular
Opportunistic Infections Matilde Ruiz-Cruz, MD, MSc; Santiago
Ávila-Rios, PhD; Christopher E. Ormsby, MSc; Claudia Alvarado-de la
Barrera, PhD;
Yuria Ablanedo-Terrazas, MD, MSc; Gustavo Reyes-Terán*, MD,
MSc.Centro de Investigación en Enfermedades Infecciosas, Instituto
Nacional de Enfermedades Respiratorias Ismael Cosío Villegas,
Mexico City, Mexico.
MATERIAL AND METHODSWe included HIV+ patients with the following
OI:23 with cytomegalovirus retinitis (CMVr), 16 withocular syphilis
and 7 with other OI. As control measurements we analyzed plasma
from 15 healthy donors and AqH from 16 patients undergoing cataract
surgery. Concentrations of 27 cytokines in AqH and plasma before
and after OI treatment were assessed using a Luminex assay.
Cytokine concentrations in AqH and plasma were compared between OI
and HIV-infected individuals vs. controls, by Wilcoxontests
corrected for multiple comparisons,
Eotaxin IL-15 IL-7G-CSF IL-17 IL-8
GM-CSF IL-1α IP-10IFN-2α IL-1β MCP-1IFN-γ IL-2 MIP-1αIL-10 IL-3
MIP-1β
IL-12p40 IL-4 RANTESIL-12p70 IL-5 TNF-α
IL-13 IL-6 TNF-β
Table 1. Cytokines and chemokines included in the Luminex panel.
Th1 cytokine, Th2, Th1/2, Th17, stim factor, chemokines,
proinflammatory, antiviral.
Figure 2. Cytokine and chemokine levels pre-treatment were
increased significantly in AqH and plasma, except for plasma
IL-12p70 and IL-17, which were decreased. Levels of GM-CSF, IL-1α
and IP-10 in were significantly higher in CMVr than syphilis
patients. C: Control, Cv: CMVr, S: Syphilis, O:Other. All cytokines
and chemokines shown have p
